Product: VCAN Antibody
Catalog: DF10007
Description: Rabbit polyclonal antibody to VCAN
Application: WB IHC
Reactivity: Human
Prediction: Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 373 kDa; 373kD(Calculated).
Uniprot: P13611
RRID: AB_2840587

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
VCAN Antibody detects endogenous levels of total VCAN.
RRID:
AB_2840587
Cite Format: Affinity Biosciences Cat# DF10007, RRID:AB_2840587.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Chondroitin sulfate proteoglycan core protein, cartilage; CSPG2; CSPG2_HUMAN; ERVR; GHAP; Glial hyaluronate binding protein; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PGM; VCAN; Versican; Versican core protein; Versican proteoglycan; WGN 1; WGN; WGN1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P13611 CSPG2_HUMAN:

Expressed in the retina (at protein level) (PubMed:29777959). Cerebral white matter and plasma (PubMed:2469524). Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas (PubMed:8627343). Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas (PubMed:8627343). Isoform V2: Restricted to normal brain and gliomas (PubMed:8627343). Isoform V3: Found in all these tissues except medulloblastomas (PubMed:8627343).

Sequence:
MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKPKEATTIDLSILAETASPSLSKEPQMVSDRTTPIIPLVDELPVIPTEFPPVGNIVSFEQKATVQPQAITDSLATKLPTPTGSTKKPWDMDDYSPSASGPLGKLDISEIKEEVLQSTTGVSHYATDSWDGVVEDKQTQESVTQIEQIEVGPLVTSMEILKHIPSKEFPVTETPLVTARMILESKTEKKMVSTVSELVTTGHYGFTLGEEDDEDRTLTVGSDESTLIFDQIPEVITVSKTSEDTIHTHLEDLESVSASTTVSPLIMPDNNGSSMDDWEERQTSGRITEEFLGKYLSTTPFPSQHRTEIELFPYSGDKILVEGISTVIYPSLQTEMTHRRERTETLIPEMRTDTYTDEIQEEITKSPFMGKTEEEVFSGMKLSTSLSEPIHVTESSVEMTKSFDFPTLITKLSAEPTEVRDMEEDFTATPGTTKYDENITTVLLAHGTLSVEAATVSKWSWDEDNTTSKPLESTEPSASSKLPPALLTTVGMNGKDKDIPSFTEDGADEFTLIPDSTQKQLEEVTDEDIAAHGKFTIRFQPTTSTGIAEKSTLRDSTTEEKVPPITSTEGQVYATMEGSALGEVEDVDLSKPVSTVPQFAHTSEVEGLAFVSYSSTQEPTTYVDSSHTIPLSVIPKTDWGVLVPSVPSEDEVLGEPSQDILVIDQTRLEATISPETMRTTKITEGTTQEEFPWKEQTAEKPVPALSSTAWTPKEAVTPLDEQEGDGSAYTVSEDELLTGSERVPVLETTPVGKIDHSVSYPPGAVTEHKVKTDEVVTLTPRIGPKVSLSPGPEQKYETEGSSTTGFTSSLSPFSTHITQLMEETTTEKTSLEDIDLGSGLFEKPKATELIEFSTIKVTVPSDITTAFSSVDRLHTTSAFKPSSAITKKPPLIDREPGEETTSDMVIIGESTSHVPPTTLEDIVAKETETDIDREYFTTSSPPATQPTRPPTVEDKEAFGPQALSTPQPPASTKFHPDINVYIIEVRENKTGRMSDLSVIGHPIDSESKEDEPCSEETDPVHDLMAEILPEFPDIIEIDLYHSEENEEEEEECANATDVTTTPSVQYINGKHLVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDTHPFVIAKTELSTAVQPNESTETTESLEVTWKPETYPETSEHFSGGEPDVFPTVPFHEEFESGTAKKGAESVTERDTEVGHQAHEHTEPVSLFPEESSGEIAIDQESQKIAFARATEVTFGEEVEKSTSVTYTPTIVPSSASAYVSEEEAVTLIGNPWPDDLLSTKESWVEATPRQVVELSGSSSIPITEGSGEAEEDEDTMFTMVTDLSQRNTTDTLITLDTSRIITESFFEVPATTIYPVSEQPSAKVVPTKFVSETDTSEWISSTTVEEKKRKEEEGTTGTASTFEVYSSTQRSDQLILPFELESPNVATSSDSGTRKSFMSLTTPTQSEREMTDSTPVFTETNTLENLGAQTTEHSSIHQPGVQEGLTTLPRSPASVFMEQGSGEAAADPETTTVSSFSLNVEYAIQAEKEVAGTLSPHVETTFSTEPTGLVLSTVMDRVVAENITQTSREIVISERLGEPNYGAEIRGFSTGFPLEEDFSGDFREYSTVSHPIAKEETVMMEGSGDAAFRDTQTSPSTVPTSVHISHISDSEGPSSTMVSTSAFPWEEFTSSAEGSGEQLVTVSSSVVPVLPSAVQKFSGTASSIIDEGLGEVGTVNEIDRRSTILPTAEVEGTKAPVEKEEVKVSGTVSTNFPQTIEPAKLWSRQEVNPVRQEIESETTSEEQIQEEKSFESPQNSPATEQTIFDSQTFTETELKTTDYSVLTTKKTYSDDKEMKEEDTSLVNMSTPDPDANGLESYTTLPEATEKSHFFLATALVTESIPAEHVVTDSPIKKEESTKHFPKGMRPTIQESDTELLFSGLGSGEEVLPTLPTESVNFTEVEQINNTLYPHTSQVESTSSDKIEDFNRMENVAKEVGPLVSQTDIFEGSGSVTSTTLIEILSDTGAEGPTVAPLPFSTDIGHPQNQTVRWAEEIQTSRPQTITEQDSNKNSSTAEINETTTSSTDFLARAYGFEMAKEFVTSAPKPSDLYYEPSGEGSGEVDIVDSFHTSATTQATRQESSTTFVSDGSLEKHPEVPSAKAVTADGFPTVSVMLPLHSEQNKSSPDPTSTLSNTVSYERSTDGSFQDRFREFEDSTLKPNRKKPTENIIIDLDKEDKDLILTITESTILEILPELTSDKNTIIDIDHTKPVYEDILGMQTDIDTEVPSEPHDSNDESNDDSTQVQEIYEAAVNLSLTEETFEGSADVLASYTQATHDESMTYEDRSQLDHMGFHFTTGIPAPSTETELDVLLPTATSLPIPRKSATVIPEIEGIKAEAKALDDMFESSTLSDGQAIADQSEIIPTLGQFERTQEEYEDKKHAGPSFQPEFSSGAEEALVDHTPYLSIATTHLMDQSVTEVPDVMEGSNPPYYTDTTLAVSTFAKLSSQTPSSPLTIYSGSEASGHTEIPQPSALPGIDVGSSVMSPQDSFKEIHVNIEATFKPSSEEYLHITEPPSLSPDTKLEPSEDDGKPELLEEMEASPTELIAVEGTEILQDFQNKTDGQVSGEAIKMFPTIKTPEAGTVITTADEIELEGATQWPHSTSASATYGVEAGVVPWLSPQTSERPTLSSSPEINPETQAALIRGQDSTIAASEQQVAARILDSNDQATVNPVEFNTEVATPPFSLLETSNETDFLIGINEESVEGTAIYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
91
Sheep
91
Pig
0
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13611 As Substrate

Site PTM Type Enzyme
K68 Acetylation
K79 Acetylation
K98 Acetylation
K98 Ubiquitination
Y208 Phosphorylation
S364 Phosphorylation
S368 Phosphorylation
S510 O-Glycosylation
T516 O-Glycosylation
T518 O-Glycosylation
T522 O-Glycosylation
T627 O-Glycosylation
T632 O-Glycosylation
S641 O-Glycosylation
T642 O-Glycosylation
T642 Phosphorylation
T643 O-Glycosylation
S647 Phosphorylation
T687 O-Glycosylation
T689 O-Glycosylation
T716 O-Glycosylation
T716 Phosphorylation
S727 Phosphorylation
T751 O-Glycosylation
T754 O-Glycosylation
T792 O-Glycosylation
T799 O-Glycosylation
S801 O-Glycosylation
T869 O-Glycosylation
T886 O-Glycosylation
T887 O-Glycosylation
S888 O-Glycosylation
T889 O-Glycosylation
S976 O-Glycosylation
T981 O-Glycosylation
T1092 O-Glycosylation
T1093 O-Glycosylation
S1101 O-Glycosylation
S1103 O-Glycosylation
T1110 O-Glycosylation
T1116 O-Glycosylation
T1121 O-Glycosylation
T1123 O-Glycosylation
S1131 O-Glycosylation
S1133 O-Glycosylation
T1191 O-Glycosylation
S1212 O-Glycosylation
T1219 O-Glycosylation
T1220 O-Glycosylation
S1221 O-Glycosylation
S1349 Phosphorylation
S1351 Phosphorylation
T1418 O-Glycosylation
T1419 O-Glycosylation
T1566 O-Glycosylation
T1569 O-Glycosylation
S1716 O-Glycosylation
T1719 O-Glycosylation
T1731 O-Glycosylation
T1734 O-Glycosylation
S1742 O-Glycosylation
S1743 O-Glycosylation
T1744 O-Glycosylation
T1763 O-Glycosylation
S1765 O-Glycosylation
T1769 O-Glycosylation
S1772 O-Glycosylation
S1775 O-Glycosylation
T1777 O-Glycosylation
T1778 O-Glycosylation
T1780 O-Glycosylation
S1909 O-Glycosylation
S1925 O-Glycosylation
T1926 O-Glycosylation
S1935 O-Glycosylation
S1942 O-Glycosylation
T1943 O-Glycosylation
S1945 O-Glycosylation
T2036 O-Glycosylation
T2050 Phosphorylation
S2058 O-Glycosylation
S2058 Phosphorylation
T2059 O-Glycosylation
T2059 Phosphorylation
T2063 O-Glycosylation
T2069 O-Glycosylation
T2083 O-Glycosylation
S2085 O-Glycosylation
T2086 O-Glycosylation
T2086 Phosphorylation
T2091 O-Glycosylation
S2099 Phosphorylation
T2115 Phosphorylation
S2116 Phosphorylation
T2152 O-Glycosylation
T2153 O-Glycosylation
T2159 O-Glycosylation
T2160 O-Glycosylation
T2163 Phosphorylation
T2194 O-Glycosylation
T2200 O-Glycosylation
S2203 O-Glycosylation
K2238 Methylation
T2331 Phosphorylation
S2337 Phosphorylation
T2416 O-Glycosylation
S2417 O-Glycosylation
S2422 O-Glycosylation
T2448 O-Glycosylation
S2455 O-Glycosylation
S2456 O-Glycosylation
T2457 O-Glycosylation
T2458 O-Glycosylation
S2473 O-Glycosylation
T2503 O-Glycosylation
T2505 O-Glycosylation
T2509 O-Glycosylation
S2515 O-Glycosylation
T2516 O-Glycosylation
S2530 O-Glycosylation
T2531 O-Glycosylation
T2540 O-Glycosylation
T2617 Phosphorylation
T2656 O-Glycosylation
S2661 O-Glycosylation
S2699 O-Glycosylation
T2701 O-Glycosylation
S2781 O-Glycosylation
T2784 O-Glycosylation
T2785 O-Glycosylation
S2941 Phosphorylation
T2950 O-Glycosylation
S3024 O-Glycosylation
T3025 O-Glycosylation
S3029 O-Glycosylation
T3205 Phosphorylation
S3206 Phosphorylation
S3209 Phosphorylation
K3312 Acetylation
S3363 Phosphorylation

Research Backgrounds

Function:

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

PTMs:

Phosphorylated by FAM20C in the extracellular medium.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix. Cell projection>Cilium>Photoreceptor outer segment. Secreted>Extracellular space>Extracellular matrix>Interphotoreceptor matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the retina (at protein level). Cerebral white matter and plasma. Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2: Restricted to normal brain and gliomas. Isoform V3: Found in all these tissues except medulloblastomas.

Subunit Structure:

Interacts with FBLN1.

Family&Domains:

Belongs to the aggrecan/versican proteoglycan family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

References

1). Establishment and functional characterization of immortalized rabbit dermal papilla cell lines. Animal biotechnology, 2023 (PubMed: 37652434) [IF=3.7]

Application: IF/ICC    Species: rabbit    Sample: DPCs and R-7 cell

Figure 2. Indirect immunofluorescence staining of primary DPCs and R-7. All the DPCs were incubated with antibodies against α-SMA, SV40LT and Versican (Scale Bar: 50 µm). Passage 5 of DPCs were used.

2). miR-543 impairs cell proliferation, migration, and invasion in breast cancer by suppressing VCAN. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 34293593) [IF=3.1]

Application: WB    Species: Human    Sample: BC cell

Fig. 4. The low expression of VCAN blocked BC cell malignancy caused by miR-543 inhibitor. (A) VCAN protein levels in MDA-MB-231 and MCF-7 treated with si-VCAN þ miR- 543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by western blotting. (B) Cell viability of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by CCK-8 assay. (C) Cell proliferation of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by BrdU assay. (D) Cell migration of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC was detected by wound healing assay. (E) Cell invasion of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC was detected by transwell assay. *P < 0.05, **P < 0.001 versus negative control group. #P < 0.05, ##P < 0.001 versus si- VCAN þ miR-543 inhibitor. Data are presented as the mean ± SD.

3). Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 36388171)

Application: IF/ICC    Species: Mouse    Sample:

Figure 1 Morphology and indirect immunofluorescence staining of HFSCs and DPCs. (a) α-SMA and Versican antibodies were incubated with DPCs (passage 2) (scale bar: 50 μm). (b) Alkaline phosphatase staining was examined with DPCs (passage 8) (scale bar: 100 μm, 50 μm). (c) KRT14, KRT15, and KRT19 antibodies were incubated with HFSCs (passage 2) (scale bar: 50 μm).

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