Product: ATP6V1G3 Antibody
Catalog: DF10005
Description: Rabbit polyclonal antibody to ATP6V1G3
Application: WB
Reactivity: Human
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 14 kDa; 14kD(Calculated).
Uniprot: Q96LB4
RRID: AB_2840585

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(88%), Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
ATP6V1G3 Antibody detects endogenous levels of total ATP6V1G3.
RRID:
AB_2840585
Cite Format: Affinity Biosciences Cat# DF10005, RRID:AB_2840585.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP6G3; atp6v1g3; ATPase, H+ transporting, lysosomal (vacuolar proton pump) subunit G3; ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3; MGC119810; MGC119813; MGC130213; MGC130214; V ATPase 13 kDa subunit 3; V ATPase G subunit 3; V ATPase G3 subunit; V ATPase subunit G 3; V-ATPase 13 kDa subunit 3; V-ATPase subunit G 3; V-type proton ATPase subunit G 3; Vacuolar ATP synthase subunit G 3; Vacuolar proton pump G subunit 3; Vacuolar proton pump subunit G 3; Vacuolar proton pump, subunit G3; VATG3_HUMAN; Vma10;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
MTSQSQGIHQLLQAEKRAKDKLEEAKKRKGKRLKQAKEEAMVEIDQYRMQRDKEFRLKQSKIMGSQNNLSDEIEEQTLGKIQELNGHYNKYMESVMNQLLSMVCDMKPEIHVNYRATN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
88
Dog
88
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96LB4 As Substrate

Site PTM Type Enzyme
K31 Acetylation
K37 Acetylation
K37 Ubiquitination

Research Backgrounds

Function:

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Tissue Specificity:

Kidney.

Subunit Structure:

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).

Family&Domains:

Belongs to the V-ATPase G subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Collecting duct acid secretion.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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