UBE2U Antibody - #DF10002

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Product: UBE2U Antibody
Catalog: DF10002
Description: Rabbit polyclonal antibody to UBE2U
Application: WB
Reactivity: Human
Prediction: Rabbit
Mol.Wt.: 38 kDa; 38kD(Calculated).
Uniprot: Q5VVX9
RRID: AB_2840582

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rabbit(88%)
Clonality:
Polyclonal
Specificity:
UBE2U Antibody detects endogenous levels of total UBE2U.
RRID:
AB_2840582
Cite Format: Affinity Biosciences Cat# DF10002, RRID:AB_2840582.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

RP4-636O23.1; UBE2U; UBE2U_HUMAN; Ubiquitin carrier protein U; Ubiquitin conjugating enzyme E2U (putative); Ubiquitin-conjugating enzyme E2 U; Ubiquitin-protein ligase U;

Immunogens

Immunogen:
Uniprot:

Q5VVX9(UBE2U_HUMAN) >>Visit HPA database.

Gene(ID):
UBE2U(148581)
Sequence:
MHGRAYLLLHRDFCDLKENNYKGITAKPVSEDMMEWEVEIEGLQNSVWQGLVFQLTIHFTSEYNYAPPVVKFITIPFHPNVDPHTGQPCIDFLDNPEKWNTNYTLSSILLALQVMLSNPVLENPVNLEAARILVKDESLYRTILRLFNRPLQMKDDSQELPKDPRKCIRPIKTTSFSDYYQTWSRIATSKATEYYRTPLLKVPNFIGQYYKWKKMDLQHQKEWNLKYSVIKCWLARKRMPHEVTHSMEEIKLCPTLIPTTDEIFLESPTAINSITDIYETEEEGWKSDTSLYENDTDEPREEEVEDLISWTNTLNTNTSED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5VVX9 As Substrate

Site PTM Type Enzyme
T173 Phosphorylation
T182 Phosphorylation

Research Backgrounds

Function:

Catalyzes the covalent attachment of ubiquitin to other proteins.

PTMs:

Autoubiquitinated in vitro in the presence of UBR5.

Family&Domains:

Belongs to the ubiquitin-conjugating enzyme family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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