Phospho-TOPBP1 (Ser1159) Antibody - #AF8517

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Product: Phospho-TOPBP1 (Ser1159) Antibody
Catalog: AF8517
Description: Rabbit polyclonal antibody to Phospho-TOPBP1 (Ser1159)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 173 kDa; 171kD(Calculated).
Uniprot: Q92547
RRID: AB_2840571

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Phospho-TOPBP1 (Ser1159) Antibody detects endogenous levels of TOPBP1 only when phosphorylated at Ser1159.
RRID:
AB_2840571
Cite Format: Affinity Biosciences Cat# AF8517, RRID:AB_2840571.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA topoisomerase 2 binding protein 1; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II binding protein 1; DNA topoisomerase II binding protein; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1; DNA topoisomerase IIbeta binding protein 1; Hypothetical protein KIAA0259 [Fragment]; KIAA0259; TOP2BP1; TOPB1_HUMAN; TOPBP 1; TopBP1; Topoisomerase (DNA) II binding protein 1; Topoisomerase II binding protein 1;

Immunogens

Immunogen:
Uniprot:

Q92547(TOPB1_HUMAN) >>Visit HPA database.

Gene(ID):
TOPBP1(11073)
Expression:
Q92547 TOPB1_HUMAN:

Highly expressed in heart, brain, placenta, lung and kidney.

Sequence:
MSRNDKEPFFVKFLKSSDNSKCFFKALESIKEFQSEEYLQIITEEEALKIKENDRSLYICDPFSGVVFDHLKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTDINMEDFKCPIFLGCIICVTGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDESIYKTEPRPEAKTMPNSSTPTSQINTIDSRTLSDVSNISNINASCVSESICNSLNSKLEPTLENLENLDVSAFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDELKQFWNKSAHRPHVVGAKWLLECFSKGYMLSEEPYIHANYQPVEIPVSHKPESKAALLKKKNSSFSKKDFAPSEKHEQADEDLLSQYENGSSTVVEAKTSEARPFNDSTHAEPLNDSTHISLQEENQSSVSHCVPDVSTITEEGLFSQKSFLVLGFSNENESNIANIIKENAGKIMSLLSRTVADYAVVPLLGCEVEATVGEVVTNTWLVTCIDYQTLFDPKSNPLFTPVPVMTGMTPLEDCVISFSQCAGAEKESLTFLANLLGASVQEYFVRKSNAKKGMFASTHLILKERGGSKYEAAKKWNLPAVTIAWLLETARTGKRADESHFLIENSTKEERSLETEITNGINLNSDTAEHPGTRLQTHRKTVVTPLDMNRFQSKAFRAVVSQHARQVAASPAVGQPLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGRPSQQKRKPSTPLSEVIVKNLQLALANSSRNAVALSASPQLKEAQSEKEEAPKPLHKVVVCVSKKLSKKQSELNGIAASLGADYRWSFDETVTHFIYQGRPNDTNREYKSVKERGVHIVSEHWLLDCAQECKHLPESLYPHTYNPKMSLDISAVQDGRLCNSRLLSAVSSTKDDEPDPLILEENDVDNMATNNKESAPSNGSGKNDSKGVLTQTLEMRENFQKQLQEIMSATSIVKPQGQRTSLSRSGCNSASSTPDSTRSARSGRSRVLEALRQSRQTVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSCPTQYSELQVDIQNLEDSPFQKPLHDSEIAKQAVCDPGNIRVTEAPKHPISEELETPIKDSHLIPTPQAPSIAFPLANPPVAPHPREKIITIEETHEELKKQYIFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGSSSILDVLTGINVQQRRLALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLPGHSVPLFKEATHLFSDLNKLKPDDSGVNIAEAAAQNVYCLRTEYIADYLMQESPPHVENYCLPEAISFIQNNKELGTGLSQKRKAPTEKNKIKRPRVH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
80
Zebrafish
80
Chicken
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92547 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
K15 Ubiquitination
K21 Ubiquitination
K31 Ubiquitination
K72 Ubiquitination
S142 Phosphorylation
T144 Phosphorylation
S153 Phosphorylation
K155 Ubiquitination
K163 Ubiquitination
K164 Ubiquitination
K178 Ubiquitination
T185 Phosphorylation
Y187 Phosphorylation
T188 Phosphorylation
K224 Ubiquitination
K234 Ubiquitination
K247 Ubiquitination
K283 Ubiquitination
K291 Ubiquitination
T298 Phosphorylation
T300 Phosphorylation
S301 Phosphorylation
S350 Phosphorylation
S370 Phosphorylation
K414 Ubiquitination
S492 Phosphorylation Q13315 (ATM)
Y494 Phosphorylation
S498 Phosphorylation
S499 Phosphorylation
S554 Phosphorylation Q13315 (ATM)
K581 Acetylation
S587 Phosphorylation
T618 Phosphorylation
S741 Phosphorylation
T742 Phosphorylation
K743 Ubiquitination
S747 Phosphorylation
T776 Phosphorylation
T779 Phosphorylation
K789 Ubiquitination
S805 Phosphorylation
S817 Phosphorylation
T822 Phosphorylation
S836 Phosphorylation
K840 Ubiquitination
T848 Phosphorylation P24941 (CDK2)
S853 Phosphorylation Q13315 (ATM)
K856 Ubiquitination
S860 Phosphorylation
T861 Phosphorylation
S864 Phosphorylation
S878 Phosphorylation
S879 Phosphorylation
S886 Phosphorylation
S888 Phosphorylation
S998 Phosphorylation
S1002 Phosphorylation
S1012 Phosphorylation
S1016 Phosphorylation
K1058 Sumoylation
K1058 Ubiquitination
T1062 Phosphorylation Q13315 (ATM)
T1064 Phosphorylation
S1093 Phosphorylation
C1099 S-Nitrosylation
S1126 Phosphorylation
S1138 Phosphorylation Q13315 (ATM)
S1159 Phosphorylation P31749 (AKT1)
S1183 Phosphorylation
S1192 Phosphorylation
S1216 Phosphorylation
T1221 Phosphorylation
T1231 Phosphorylation
K1253 Ubiquitination
K1265 Ubiquitination
K1289 Ubiquitination
K1297 Ubiquitination
K1317 Ubiquitination
K1412 Methylation
K1421 Ubiquitination
K1432 Ubiquitination
K1443 Ubiquitination
K1445 Ubiquitination
S1504 Phosphorylation
K1506 Ubiquitination

Research Backgrounds

Function:

Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.

PTMs:

Phosphorylated on serine and threonine residues in response to X-ray irradiation.

Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.

Subcellular Location:

Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole. Chromosome.
Note: Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart, brain, placenta, lung and kidney.

Subunit Structure:

Interacts with POLE. Interacts with RAD9A. Interacts with UBR5. Interacts with E2F1. Interacts with PML. Interacts with SMARCA2. Interacts with SMARCA4. Interacts with RHNO1. May interact with TOP2B. Interacts with TICRR. Interacts with HELB.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

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