Phospho-R-Ras (Tyr66) Antibody - #AF8509

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Product: Phospho-R-Ras (Tyr66) Antibody
Catalog: AF8509
Description: Rabbit polyclonal antibody to Phospho-R-Ras (Tyr66)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Dog
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: P10301
RRID: AB_2840563

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-R-Ras (Tyr66) Antibody detects endogenous levels of R-Ras only when phosphorylated at Tyr66.
RRID:
AB_2840563
Cite Format: Affinity Biosciences Cat# AF8509, RRID:AB_2840563.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Oncogene RRAS; p23; R Ras; Ras related Protein; Ras related protein R Ras; Ras-related protein R-Ras; related RAS viral (r ras) oncogene homolog; RRAS; RRAS_HUMAN;

Immunogens

Immunogen:
Uniprot:

P10301(RRAS_HUMAN) >>Visit HPA database.

Gene(ID):
RRAS(6237)
Sequence:
MSSGAASGTGRGRPRGGGPGPGDPPPSETHKLVVVGGGGVGKSALTIQFIQSYFVSDYDPTIEDSYTKICSVDGIPARLDILDTAGQEEFGAMREQYMRAGHGFLLVFAINDRQSFNEVGKLFTQILRVKDRDDFPVVLVGNKADLESQRQVPRSEASAFGASHHVAYFEASAKLRLNVDEAFEQLVRAVRKYQEQELPPSPPSAPRKKGGGCPCVLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Horse
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10301 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
R11 Methylation
K31 Ubiquitination
Y58 Phosphorylation
Y66 Phosphorylation P29323 (EPHB2) , P12931 (SRC)
K121 Ubiquitination
K143 Ubiquitination
K192 Ubiquitination
S201 Phosphorylation
S204 Phosphorylation

Research Backgrounds

Function:

Regulates the organization of the actin cytoskeleton. With OSPBL3, modulates integrin beta-1 (ITGB1) activity.

PTMs:

S-palmitoylated by ZDHHC19, leading to increased association with membranes and with rafts/caveolae as well as enhanced cell viability.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.
Note: Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PLCE1. Interacts (active GTP-bound form preferentially) with RGS14 (By similarity). Interacts with OSBPL3 (By similarity). Interacts with ZDHHC19.

Family&Domains:

Belongs to the small GTPase superfamily. Ras family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). Optimized new Shengmai powder ameliorates myocardial fibrosis in rats with heart failure by inhibition of the MAPK signaling pathway. Journal of ethnopharmacology, 2024 (PubMed: 37739104) [IF=5.4]

Application: WB    Species: Rat    Sample:

Fig. 7. Effects of ONSMP on ERK1/2 signaling pathway. (A) Representative western blotting bands of p-RAS, RAS, p-RAF, RAF, p-MEK1/2, MEK1/2, p-ERK1/2, ERK1/2, p-ELK, ELK, and c-Fos in the myocardium, with vinculin as internal reference. (B–G) Statistical graphs of p-RAS/RAS, p-RAF/RAF, p-MEK1/2/MEK1/2, p-ERK1/2/ERK1/2, p-ELK/ELK, and c-Fos (n = 3). Data are presented as mean ± SEM. nsP > 0.05; ▲▲P < 0.01 vs the sham; **P < 0.01 vs the model; #P < 0.05, ##P < 0.01 vs the ONSMP-L; ※P < 0.05, ※※P < 0.01 vs the ONSMP-M.
2). Optimized new Shengmai powder inhibits myocardial fibrosis in heart failure by regulating the rat sarcoma/rapidly accelerated fibrosarcoma/mitogen-activated protein kinase kinase/extracellular regulated protein kinases signaling pathway. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 2024 (PubMed: 38767628) [IF=2.6]

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