Phospho-PNKP (Ser114) Antibody | Affinity Biosciences

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Product:	Phospho-PNKP (Ser114) Antibody
Catalog:	AF8503
Description:	Rabbit polyclonal antibody to Phospho-PNKP (Ser114)
Application:	WB
Reactivity:	Human
Mol.Wt.:	60 kDa; 57kD(Calculated).
Uniprot:	Q96T60
RRID:	AB_2840557

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-PNKP (Ser114) Antibody detects endogenous levels of PNKP only when phosphorylated at Ser114.

RRID:

AB_2840557
Cite Format: Affinity Biosciences Cat# AF8503, RRID:AB_2840557.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2''(3'')-polynucleotidase; 2'(3')-polynucleotidase; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; DNA 5' kinase/3' phosphatase; DNA 5''-kinase/3''-phosphatase; EIEE10; Homo sapiens polynucleotide kinase 3' phosphatase (PNKP); MCSZ; PNK 1; PNK1; Pnkp; PNKP DNA kinase; PNKP_HUMAN; Polynucleotide 3'-phosphatase; Polynucleotide 5' hydroxyl kinase; Polynucleotide 5''-hydroxyl-kinase; Polynucleotide kinase 3 prime phosphatase; Polynucleotide kinase 3' phosphatase; Polynucleotide Kinase; Polynucleotide kinase-3''-phosphatase;

Immunogens

Immunogen:

Uniprot:

Q96T60(PNKP_HUMAN) >>Visit HPA database.

Gene(ID):

PNKP(11284)

Expression:

Q96T60 PNKP_HUMAN:

Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).

Sequence:

Q96T60 PNKP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

PTMs - Q96T60 As Substrate

Q96T60

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S14	Phosphorylation		Uniprot
T109	Phosphorylation		Uniprot
T111	Phosphorylation		Uniprot
S114	Phosphorylation	Q13315 (ATM) , P78527 (PRKDC)	Uniprot
T118	Phosphorylation		Uniprot
T122	Phosphorylation		Uniprot
S126	Phosphorylation	P78527 (PRKDC) , Q13315 (ATM)	Uniprot
K142	Ubiquitination		Uniprot
S143	Phosphorylation		Uniprot
K183	Acetylation		Uniprot
K183	Sumoylation		Uniprot
K183	Ubiquitination		Uniprot
R224	Methylation		Uniprot
K226	Acetylation		Uniprot
K226	Ubiquitination		Uniprot
S364	Phosphorylation		Uniprot
S379	Phosphorylation		Uniprot
K484	Ubiquitination		Uniprot

Research Backgrounds

Q96T60_PNKP_HUMAN

Function:

Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine. Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver.

Subunit Structure:

Monomer.

Family&Domains:

The FHA domain binds threonine-phosphorylated peptides from XRCC1/4, and is responsible for the recruitment of PNKP to the sites of DNA repair. The affinity is ten times greater if peptides are also phosphorylated on the serine preceeding the phosphothreonine.

In the N-terminal section; belongs to the DNA 3' phosphatase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-PNKP (Ser114) Antibody - #AF8503