Phospho-PDE3A (Ser312) Antibody - #AF8501

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Product: Phospho-PDE3A (Ser312) Antibody
Catalog: AF8501
Description: Rabbit polyclonal antibody to Phospho-PDE3A (Ser312)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Rabbit, Chicken
Mol.Wt.: 125 kDa; 125kD(Calculated).
Uniprot: Q14432
RRID: AB_2840555

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Horse(100%), Rabbit(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PDE3A (Ser312) Antibody detects endogenous levels of PDE3A only when phosphorylated at Ser312.
RRID:
AB_2840555
Cite Format: Affinity Biosciences Cat# AF8501, RRID:AB_2840555.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5''-cyclic phosphodiesterase A; cAMP phosphodiesterase myocardial cGMP inhibited; CGI PDE; CGI PDE A; CGI-PDE A; cGMP inhibited 3' 5' cyclic phosphodiesterase A; cGMP-inhibited 3''; Cyclic GMP inhibited phosphodiesterase A; Cyclic GMP-inhibited phosphodiesterase A; Human cGMP inhibited cAMP phosphodiesterase mRNA; PDE 3A; Pde3a; PDE3A_HUMAN; Phosphodiesterase 3A; Phosphodiesterase 3A cGMP inhibited;

Immunogens

Immunogen:
Uniprot:

Q14432(PDE3A_HUMAN) >>Visit HPA database.

Gene(ID):
PDE3A(5139)
Sequence:
MAVPGDAARVRDKPVHSGVSQAPTAGRDCHHRADPASPRDSGCRGCWGDLVLQPLRSSRKLSSALCAGSLSFLLALLVRLVRGEVGCDLEQCKEAAAAEEEEAAPGAEGGVFPGPRGGAPGGGARLSPWLQPSALLFSLLCAFFWMGLYLLRAGVRLPLAVALLAACCGGEALVQIGLGVGEDHLLSLPAAGVVLSCLAAATWLVLRLRLGVLMIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILPQSAEAAPREHLGSQLIAGTKEDIPVFKRRRRSSSVVSAEMSGCSSKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Chicken
100
Rabbit
100
Zebrafish
83
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14432 As Substrate

Site PTM Type Enzyme
K287 Ubiquitination
S292 Phosphorylation P17252 (PRKCA)
S293 Phosphorylation P17252 (PRKCA)
S294 Phosphorylation
S297 Phosphorylation
S301 Phosphorylation
S312 Phosphorylation P17612 (PRKACA) , P17252 (PRKCA)
T399 Phosphorylation
S400 Phosphorylation
S402 Phosphorylation
S408 Phosphorylation
S410 Phosphorylation
S413 Phosphorylation
K418 Ubiquitination
S428 Phosphorylation P17612 (PRKACA) , P17252 (PRKCA)
S438 Phosphorylation P17252 (PRKCA)
S439 Phosphorylation
T440 Phosphorylation
T442 Phosphorylation
T443 Phosphorylation
T444 Phosphorylation
T445 Phosphorylation
S463 Phosphorylation
S465 Phosphorylation P17252 (PRKCA)
K467 Ubiquitination
S475 Phosphorylation
S478 Phosphorylation
K489 Ubiquitination
S492 Phosphorylation P17252 (PRKCA)
T494 Phosphorylation
Y497 Phosphorylation
K506 Ubiquitination
K518 Ubiquitination
S520 Phosphorylation
S523 Phosphorylation
S524 Phosphorylation
S527 Phosphorylation
S528 Phosphorylation
T533 Phosphorylation
K541 Ubiquitination
S550 Phosphorylation
T553 Phosphorylation
T554 Phosphorylation
K556 Ubiquitination
S558 Phosphorylation
S561 Phosphorylation
T568 Phosphorylation
S570 Phosphorylation
S575 Phosphorylation
T580 Phosphorylation
S593 Phosphorylation
S613 Phosphorylation
T615 Phosphorylation
T618 Phosphorylation
T643 Phosphorylation
S654 Phosphorylation
K729 Ubiquitination
K912 Ubiquitination
K919 Ubiquitination
K923 Ubiquitination
K947 Ubiquitination
K956 Ubiquitination
K1088 Ubiquitination
S1103 Phosphorylation
T1107 Phosphorylation
K1120 Ubiquitination
T1136 Phosphorylation

Research Backgrounds

Function:

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Endocrine system > Renin secretion.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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