Phospho-SRC3 (Ser857) Antibody | Affinity Biosciences

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Product:	Phospho-SRC3 (Ser857) Antibody
Catalog:	AF8491
Description:	Rabbit polyclonal antibody to Phospho-SRC3 (Ser857)
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Dog
Mol.Wt.:	155kDa; 155kD(Calculated).
Uniprot:	Q9Y6Q9
RRID:	AB_2840545

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-SRC3 (Ser857) Antibody detects endogenous levels of SRC3 only when phosphorylated at Ser857.

RRID:

AB_2840545
Cite Format: Affinity Biosciences Cat# AF8491, RRID:AB_2840545.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACTR; AIB 1; AIB-1; AIB1; Amplified in breast cancer 1; Amplified in breast cancer 1 protein; bHLHe42; CAGH 16; CAGH16; CBP interacting protein; CBP-interacting protein; Class E basic helix-loop-helix protein 42; CTG 26; CTG26; KAT13B / AIB1; MGC141848; NCOA 3; NCoA-3; Ncoa3; NCOA3_HUMAN; Nuclear receptor coactivator 3; pCIP; RAC 3; RAC-3; RAC3; Receptor associated coactivator 3; Receptor-associated coactivator 3; SRC 3; SRC-3; SRC3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM 1; TRAM-1; TRAM1;

Immunogens

Immunogen:

Uniprot:

Q9Y6Q9(NCOA3_HUMAN) >>Visit HPA database.

Gene(ID):

NCOA3(8202)

Expression:

Q9Y6Q9 NCOA3_HUMAN:

Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

Sequence:

Q9Y6Q9 NCOA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Chicken

Rabbit

Zebrafish

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6Q9 As Substrate

Q9Y6Q9

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
T24	Phosphorylation	P45983 (MAPK8) , Q16539 (MAPK14)	Uniprot
T30	Phosphorylation		Uniprot
S32	Phosphorylation		Uniprot
K35	Acetylation		Uniprot
K65	Ubiquitination		Uniprot
K97	Ubiquitination		Uniprot
S101	Phosphorylation		Uniprot
S102	Phosphorylation	P49674 (CSNK1E)	Uniprot
K167	Ubiquitination		Uniprot
T184	Phosphorylation		Uniprot
K191	Ubiquitination		Uniprot
S214	Phosphorylation		Uniprot
S244	Phosphorylation		Uniprot
R251	Methylation		Uniprot
S274	Phosphorylation		Uniprot
K276	Acetylation		Uniprot
T282	Phosphorylation		Uniprot
S284	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
S288	Phosphorylation		Uniprot
S306	Phosphorylation		Uniprot
S505	Phosphorylation	P45983 (MAPK8) , Q16539 (MAPK14)	Uniprot
S509	Phosphorylation		Uniprot
S543	Phosphorylation	P45983 (MAPK8) , Q16539 (MAPK14)	Uniprot
S551	Phosphorylation		Uniprot
T557	Phosphorylation		Uniprot
S563	Phosphorylation		Uniprot
S569	Phosphorylation		Uniprot
Y574	Phosphorylation		Uniprot
S601	Phosphorylation	P48730 (CSNK1D)	Uniprot
K616	Acetylation		Uniprot
K619	Acetylation		Uniprot
K620	Acetylation		Uniprot
K620	Ubiquitination		Uniprot
S636	Phosphorylation		Uniprot
S640	Phosphorylation		Uniprot
S664	Phosphorylation		Uniprot
K687	Acetylation		Uniprot
S694	Phosphorylation		Uniprot
T714	Phosphorylation		Uniprot
S715	Phosphorylation		Uniprot
K723	Sumoylation		Uniprot
S728	Phosphorylation	P06493 (CDK1)	Uniprot
K753	Ubiquitination		Uniprot
K786	Sumoylation		Uniprot
S794	Phosphorylation		Uniprot
S836	Phosphorylation		Uniprot
K840	Methylation		Uniprot
R849	Methylation		Uniprot
S857	Phosphorylation	O15111 (CHUK) , Q16659 (MAPK6) , P17612 (PRKACA)	Uniprot
S860	Phosphorylation	Q16539 (MAPK14) , P45983 (MAPK8)	Uniprot
S863	Phosphorylation		Uniprot
S866	Phosphorylation		Uniprot
S867	Phosphorylation	P06493 (CDK1) , Q16539 (MAPK14) , P45983 (MAPK8)	Uniprot
K926	Ubiquitination		Uniprot
R951	Methylation		Uniprot
S1033	Phosphorylation	Q05513 (PRKCZ)	Uniprot
S1042	Phosphorylation	Q05513 (PRKCZ)	Uniprot
S1048	Phosphorylation	Q05513 (PRKCZ)	Uniprot
T1059	Phosphorylation	Q05513 (PRKCZ)	Uniprot
S1062	Phosphorylation	Q05513 (PRKCZ)	Uniprot
T1064	Phosphorylation	Q05513 (PRKCZ)	Uniprot
T1067	Phosphorylation	Q05513 (PRKCZ)	Uniprot
K1091	Methylation		Uniprot
T1114	Phosphorylation	Q05513 (PRKCZ)	Uniprot
R1171	Methylation		Uniprot
R1177	Methylation		Uniprot
R1188	Methylation		Uniprot
K1194	Sumoylation		Uniprot
S1330	Phosphorylation		Uniprot
Y1357	Phosphorylation	P00519 (ABL1)	Uniprot

Research Backgrounds

Q9Y6Q9_NCOA3_HUMAN

Function:

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

PTMs:

Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.

Methylated by CARM1.

Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

Tissue Specificity:

Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

Subunit Structure:

Interacts with CARM1 (By similarity). Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP. Interacts with CASP8AP2, NR3C1 and PCAF. Interacts with ATAD2 and this interaction is enhanced by estradiol. Found in a complex containing NCOA3, AR and MAK. Interacts with DDX5. Interacts with PSMB9. Interacts with NPAS2. Interacts with NR4A3 (By similarity). Interacts with ESRRB; mediates the interaction between ESRRB and RNA polymerase II complexes and allows NCOA3 corecruitment to ESRRB, KLF4, NANOG, and SOX2 enhancer regions to trigger ESRRB-dependent gene activation involved in self-renewal and pluripotency (By similarity).

Family&Domains:

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

Belongs to the SRC/p160 nuclear receptor coactivator family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

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Phospho-SRC3 (Ser857) Antibody - #AF8491