Phospho-CBX1 / HP1 beta (Thr51) Antibody - #AF8483

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Product: Phospho-CBX1 / HP1 beta (Thr51) Antibody
Catalog: AF8483
Description: Rabbit polyclonal antibody to Phospho-CBX1 / HP1 beta (Thr51)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 25 kDa; 21kD(Calculated).
Uniprot: P83916
RRID: AB_2840537

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
Phospho-CBX1 / HP1 beta (Thr51) Antibody detects endogenous levels of CBX1 / HP1 beta only when phosphorylated at Thr51.
RRID:
AB_2840537
Cite Format: Affinity Biosciences Cat# AF8483, RRID:AB_2840537.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CBX 1; CBX; Cbx1; CBX1_HUMAN; Chromobox 1; Chromobox homolog 1 (HP1 beta homolog Drosophila ); chromobox homolog 1; Chromobox protein homolog 1; Drosophila HP1 beta; Heterochromatin protein 1 beta; Heterochromatin protein 1 homolog beta; Heterochromatin protein p25; heterochromatin protein p25 beta; HP1 beta; HP1 beta homolog; HP1 beta homolog Drosophila; HP1, Drosophila. homolog of, beta; HP1beta; HP1Hs beta; HP1Hsbeta; Human heterochromatin protein p25 mRNA complete cds; M31; MOD 1; MOD1; Modifier 1 protein; p25beta;

Immunogens

Immunogen:
Uniprot:

P83916(CBX1_HUMAN) >>Visit HPA database.

Gene(ID):
CBX1(10951)
Expression:
P83916 CBX1_HUMAN:

Expressed in all adult and embryonic tissues.

Sequence:
MGKKQNKKKVEEVLEEEEEEYVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESKPKKKKEESEKPRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPSEDDDKKDDKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P83916 As Substrate

Site PTM Type Enzyme
K9 Acetylation
K9 Methylation
K9 Ubiquitination
Y21 Phosphorylation
K25 Ubiquitination
K33 Acetylation
K35 Acetylation
K35 Methylation
K35 Ubiquitination
K41 Acetylation
K41 Ubiquitination
K43 Ubiquitination
T51 Phosphorylation P68400 (CSNK2A1)
S70 Phosphorylation
K72 Ubiquitination
T73 Phosphorylation
T77 Phosphorylation
S80 Phosphorylation
K84 Acetylation
K84 Ubiquitination
S89 Phosphorylation
S91 Phosphorylation
S98 Phosphorylation
K104 Ubiquitination
K109 Ubiquitination
R111 Methylation
R115 Methylation
S128 Phosphorylation
S129 Phosphorylation
K139 Acetylation
K139 Ubiquitination
K150 Ubiquitination
K155 Ubiquitination
C156 S-Nitrosylation
S172 Phosphorylation
Y173 Phosphorylation
S175 Phosphorylation
K180 Ubiquitination
K181 Ubiquitination
K184 Ubiquitination

Research Backgrounds

Function:

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

PTMs:

Not phosphorylated.

Ubiquitinated.

Subcellular Location:

Nucleus.
Note: Unassociated with chromosomes during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all adult and embryonic tissues.

Subunit Structure:

Homodimer (By similarity). Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain. Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain (By similarity). Interacts with SUV39H1 and SETDB1, KMT5B and KMT5C. Interacts with PRDM6 (By similarity). Interacts with POGZ. Interacts with CHAMP1. Interacts with INCENP. Interacts with SGO1; the CBX1 homodimer binds to one molecule of SGO1. Interacts with LRIF1 (via PxVxL motif).

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