Phospho-GFAP (Ser13) Antibody | Affinity Biosciences

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Product:	Phospho-GFAP (Ser13) Antibody
Catalog:	AF8479
Description:	Rabbit polyclonal antibody to Phospho-GFAP (Ser13)
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Rabbit
Mol.Wt.:	50kDa; 50kD(Calculated).
Uniprot:	P14136
RRID:	AB_2840533

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(90%), Horse(90%), Rabbit(80%)

Clonality:

Polyclonal

Specificity:

Phospho-GFAP (Ser13) Antibody detects endogenous levels of GFAP only when phosphorylated at Ser13.

RRID:

AB_2840533
Cite Format: Affinity Biosciences Cat# AF8479, RRID:AB_2840533.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

wu:fb34h11; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; GFAP; GFAP_HUMAN; gfapl; Glial fibrillary acidic protein; Intermediate filament protein; wu:fk42c12; xx:af506734; zgc:110485;

Immunogens

Immunogen:

Uniprot:

P14136(GFAP_HUMAN) >>Visit HPA database.

Gene(ID):

GFAP(2670)

Expression:

P14136 GFAP_HUMAN:

Expressed in cells lacking fibronectin.

Sequence:

P14136 GFAP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

Bovine

Rabbit

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14136 As Substrate

P14136

Site	PTM Type	Enzyme	Source
T7	Phosphorylation	Q96GD4 (AURKB) , P17612 (PRKACA) , Q13464 (ROCK1)	Uniprot
S8	Phosphorylation	P17612 (PRKACA)	Uniprot
R11	Methylation		Uniprot
R12	Methylation		Uniprot
S13	Phosphorylation	Q13464 (ROCK1) , P17612 (PRKACA) , Q96GD4 (AURKB)	Uniprot
Y14	Phosphorylation		Uniprot
S17	Phosphorylation		Uniprot
S38	Phosphorylation	Q96GD4 (AURKB) , Q13464 (ROCK1) , P17612 (PRKACA)	Uniprot
K95	Acetylation		Uniprot
Y116	Phosphorylation		Uniprot
T131	Phosphorylation		Uniprot
T150	Phosphorylation		Uniprot
K154	Acetylation		Uniprot
Y172	Phosphorylation		Uniprot
K189	Acetylation		Uniprot
K228	Acetylation		Uniprot
T240	Phosphorylation		Uniprot
Y242	Phosphorylation		Uniprot
K260	Acetylation		Uniprot
S305	Phosphorylation		Uniprot
Y324	Phosphorylation		Uniprot
K339	Acetylation		Uniprot
Y349	Phosphorylation		Uniprot

Research Backgrounds

P14136_GFAP_HUMAN

Function:

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

PTMs:

Phosphorylated by PKN1.

Subcellular Location:

Cytoplasm.
Note: Associated with intermediate filaments.

Tissue Specificity:

Expressed in cells lacking fibronectin.

Subunit Structure:

Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-GFAP (Ser13) Antibody - #AF8479