Phospho-EIF5 (Ser389+Ser390) Antibody - #AF8473

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Product: Phospho-EIF5 (Ser389+Ser390) Antibody
Catalog: AF8473
Description: Rabbit polyclonal antibody to Phospho-EIF5 (Ser389+Ser390)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 49kDa; 49kD(Calculated).
Uniprot: P55010
RRID: AB_2840527

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(90%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-EIF5 (Ser389+Ser390) Antibody detects endogenous levels of EIF5 only when phosphorylated at Ser389+Ser390.
RRID:
AB_2840527
Cite Format: Affinity Biosciences Cat# AF8473, RRID:AB_2840527.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810011H21Rik; D12Ertd549e; EIF 5; EIF 5A; eIF-5; Eif5; Eukaryotic initiation factor 5; Eukaryotic translation initiation factor 5; IF5_HUMAN; MGC36374; MGC36509;

Immunogens

Immunogen:
Uniprot:

P55010(IF5_HUMAN) >>Visit HPA database.

Gene(ID):
EIF5(1983)
Sequence:
MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
90
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55010 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S8 Phosphorylation
S10 Phosphorylation
Y14 Phosphorylation
Y16 Phosphorylation
K24 Acetylation
K24 Ubiquitination
K28 Acetylation
K33 Acetylation
K55 Acetylation
K55 Ubiquitination
K70 Ubiquitination
K95 Ubiquitination
K114 Ubiquitination
K115 Ubiquitination
S121 Phosphorylation
T139 Phosphorylation
S149 Phosphorylation
S151 Phosphorylation
S174 Phosphorylation P67870 (CSNK2B) , P68400 (CSNK2A1)
S175 Phosphorylation
S176 Phosphorylation
T178 Phosphorylation
T207 Phosphorylation P68400 (CSNK2A1)
T208 Phosphorylation P68400 (CSNK2A1)
S220 Phosphorylation
T227 Phosphorylation
S229 Phosphorylation
K261 Ubiquitination
K288 Ubiquitination
Y362 Phosphorylation
K369 Acetylation
S389 Phosphorylation P67870 (CSNK2B) , P68400 (CSNK2A1)
S390 Phosphorylation P68400 (CSNK2A1) , P67870 (CSNK2B)
Y405 Phosphorylation
S406 Phosphorylation
S410 Phosphorylation
T416 Phosphorylation
S419 Phosphorylation

Research Backgrounds

Function:

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Subunit Structure:

Interacts with FMR1 isoform 6; this interaction occurs in a RNA-dependent manner.

Family&Domains:

Belongs to the eIF-2-beta/eIF-5 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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