Phospho-EIF2S2 (Ser67) Antibody - #AF8472

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Product: Phospho-EIF2S2 (Ser67) Antibody
Catalog: AF8472
Description: Rabbit polyclonal antibody to Phospho-EIF2S2 (Ser67)
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40 KD; 38kD(Calculated).
Uniprot: P20042
RRID: AB_2840526

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(90%), Zebrafish(89%), Bovine(90%), Horse(90%), Sheep(80%), Rabbit(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
Phospho-EIF2S2 (Ser67) Antibody detects endogenous levels of EIF2S2 only when phosphorylated at Ser67.
RRID:
AB_2840526
Cite Format: Affinity Biosciences Cat# AF8472, RRID:AB_2840526.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686L18198; eIF 2 beta; eIF-2-beta; EIF2; EIF2B; EIF2beta; EIF2S2; Eukaryotic initiation factor 2 beta; eukaryotic initiation factor 2-beta; Eukaryotic translation initiation factor 2 beta; Eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit 2 beta 38kDa; Eukaryotic translation initiation factor 2 subunit 2 beta; Eukaryotic translation initiation factor 2 subunit beta; eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa; IF2B_HUMAN; MGC8508; PPP1R67; protein phosphatase 1, regulatory subunit 67;

Immunogens

Immunogen:
Uniprot:

P20042(IF2B_HUMAN) >>Visit HPA database.

Gene(ID):
EIF2S2(8894)
Sequence:
MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Dog
90
Rabbit
90
Zebrafish
89
Sheep
80
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20042 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S2 Acetylation
S2 Phosphorylation P68400 (CSNK2A1)
T11 Phosphorylation
S13 Phosphorylation
T31 Phosphorylation
T33 Phosphorylation
T36 Phosphorylation
S39 Phosphorylation
K52 Ubiquitination
K64 Ubiquitination
S67 Phosphorylation P68400 (CSNK2A1)
K79 Ubiquitination
K87 Ubiquitination
K99 Ubiquitination
S105 Phosphorylation
T111 Phosphorylation
K124 Methylation
K124 Ubiquitination
K125 Ubiquitination
K132 Ubiquitination
K142 Ubiquitination
K153 Ubiquitination
S158 Phosphorylation
R172 Methylation
T175 Phosphorylation
Y176 Phosphorylation
K190 Acetylation
K190 Ubiquitination
K199 Ubiquitination
K205 Ubiquitination
K216 Ubiquitination
S218 Phosphorylation P17612 (PRKACA)
C226 S-Nitrosylation
K227 Acetylation
K227 Ubiquitination
K265 Acetylation
K265 Ubiquitination
K276 Acetylation
K276 Ubiquitination
Y278 Phosphorylation
T283 Phosphorylation
S286 Phosphorylation
K293 Acetylation
K293 Ubiquitination
R296 Methylation
Y298 Phosphorylation
S310 Phosphorylation
K315 Ubiquitination
K324 Acetylation
K324 Ubiquitination

Research Backgrounds

Function:

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Subunit Structure:

Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity).

Family&Domains:

Belongs to the eIF-2-beta/eIF-5 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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