Phospho-C5R1 (Ser327) Antibody - #AF8468

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Product: Phospho-C5R1 (Ser327) Antibody
Catalog: AF8468
Description: Rabbit polyclonal antibody to Phospho-C5R1 (Ser327)
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: P21730
RRID: AB_2840522

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%), Bovine(89%), Sheep(89%), Rabbit(80%)
Clonality:
Polyclonal
Specificity:
Phospho-C5R1 (Ser327) Antibody detects endogenous levels of C5R1 only when phosphorylated at Ser327.
RRID:
AB_2840522
Cite Format: Affinity Biosciences Cat# AF8468, RRID:AB_2840522.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANAPHYLATOXIN RECEPTOR; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin receptor; C5A; C5a ligand; C5a-R; C5aR; C5AR_HUMAN; c5ar1; C5R1; CD88; CD88 antigen; Complement component 5 receptor 1; Complement component 5 receptor 2; Complement component 5a receptor 1;

Immunogens

Immunogen:
Uniprot:

P21730(C5AR1_HUMAN) >>Visit HPA database.

Gene(ID):
C5AR1(728)
Sequence:
MDSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRVPDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTINAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFGGAACSILPSLILLNMYASILLLATISADRFLLVFKPIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYRVVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLGFLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVVAVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLKKLDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSLPSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Bovine
89
Sheep
89
Rabbit
80
Xenopus
67
Horse
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21730 As Substrate

Site PTM Type Enzyme
Y11 Phosphorylation
Y14 Phosphorylation
S314 Phosphorylation P17252 (PRKCA)
S317 Phosphorylation P17252 (PRKCA)
T324 Phosphorylation
S327 Phosphorylation P17252 (PRKCA)
S332 Phosphorylation P17252 (PRKCA)
S334 Phosphorylation Q05655 (PRKCD) , P17252 (PRKCA) , P05771-2 (PRKCB) , P05129 (PRKCG)
S338 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.

PTMs:

Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist. Sulfation at Tyr-14 is important for CHIPS binding.

Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cytoplasmic vesicle.
Note: Phosphorylated C5aR colocalizes with ARRB1 and ARRB2 in cytoplasmic vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. May also form higher-order oligomers. Interacts (when phosphorylated) with ARRB1 and ARRB2; the interaction is associated with internalization of C5aR. Interacts (via N-terminal domain) with S.aureus chemotaxis inhibitory protein (CHIPS); the interaction blocks the receptor and may thus inhibit the immune response.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

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