Phospho-Mre11 (Ser676) Antibody | Affinity Biosciences

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Product:	Phospho-Mre11 (Ser676) Antibody
Catalog:	AF8445
Description:	Rabbit polyclonal antibody to Phospho-Mre11 (Ser676)
Application:	WB
Reactivity:	Human
Mol.Wt.:	80 kDa; 81kD(Calculated).
Uniprot:	P49959
RRID:	AB_2840502

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Phospho-Mre11 (Ser676) Antibody detects endogenous levels of Mre11 only when phosphorylated at Ser676.

RRID:

AB_2840502
Cite Format: Affinity Biosciences Cat# AF8445, RRID:AB_2840502.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AT like disease; Ataxia telangiectasia disorder like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; meiotic recombination (S. cerevisiae) 11 homolog A; Meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A (S. cerevisiae); Meiotic recombination 11 homolog A; MmMRE11A; Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); MRE11 meiotic recombination 11 homolog A; MRE11_HUMAN; MRE11A; MRE11b; OTTHUMP00000236830; OTTHUMP00000236831; OTTHUMP00000236832; OTTHUMP00000236833;

Immunogens

Immunogen:

Uniprot:

P49959(MRE11_HUMAN) >>Visit HPA database.

Gene(ID):

MRE11(4361)

Sequence:

P49959 MRE11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEEINFGKLITKPSEGTTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERHIDALEDKIDEEVRRFRETRQKNTNEEDDEVREAMTRARALRSQSEESASAFSADDLMSIDLAEQMANDSDDSISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRADTGLETSTRSRNSKTAVSASRNMSIIDAFKSTRQQPSRNVTTKNYSEVIEVDESDVEEDIFPTTSKTDQRWSSTSSSKIMSQSQVSKGVDFESSEDDDDDPFMNTSSLRRNRR

PTMs - P49959 As Substrate

P49959

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
T12	Phosphorylation		Uniprot
K70	Ubiquitination		Uniprot
T74	Phosphorylation		Uniprot
S158	Phosphorylation		Uniprot
K161	Ubiquitination		Uniprot
S165	Phosphorylation		Uniprot
K171	Ubiquitination		Uniprot
S183	Phosphorylation		Uniprot
Y190	Phosphorylation		Uniprot
K196	Acetylation		Uniprot
K197	Acetylation		Uniprot
K197	Ubiquitination		Uniprot
K255	Ubiquitination		Uniprot
S264	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
T272	Phosphorylation		Uniprot
S273	Phosphorylation		Uniprot
S275	Phosphorylation		Uniprot
K282	Ubiquitination		Uniprot
K298	Ubiquitination		Uniprot
T329	Phosphorylation		Uniprot
K339	Ubiquitination		Uniprot
K360	Ubiquitination		Uniprot
S382	Phosphorylation	Q13535 (ATR)	Uniprot
K384	Ubiquitination		Uniprot
R388	Methylation		Uniprot
K393	Ubiquitination		Uniprot
K407	Ubiquitination		Uniprot
K416	Acetylation		Uniprot
K416	Ubiquitination		Uniprot
K420	Ubiquitination		Uniprot
T426	Phosphorylation		Uniprot
K434	Ubiquitination		Uniprot
Y436	Phosphorylation		Uniprot
K442	Ubiquitination		Uniprot
S447	Phosphorylation		Uniprot
K464	Ubiquitination		Uniprot
K475	Ubiquitination		Uniprot
K480	Acetylation		Uniprot
K480	Ubiquitination		Uniprot
T481	Phosphorylation		Uniprot
K486	Ubiquitination		Uniprot
K496	Ubiquitination		Uniprot
S531	Phosphorylation	Q13535 (ATR)	Uniprot
S558	Phosphorylation		Uniprot
S561	Phosphorylation		Uniprot
R570	Methylation		Uniprot
R572	Methylation		Uniprot
R574	Methylation		Uniprot
R576	Methylation		Uniprot
R577	Methylation		Uniprot
R580	Methylation		Uniprot
R587	Methylation		Uniprot
S590	Phosphorylation		Uniprot
R592	Methylation		Uniprot
R594	Methylation		Uniprot
T597	Phosphorylation		Uniprot
R604	Methylation		Uniprot
S613	Phosphorylation		Uniprot
R616	Methylation		Uniprot
S619	Phosphorylation		Uniprot
K625	Sumoylation		Uniprot
S649	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S667	Phosphorylation		Uniprot
S668	Phosphorylation		Uniprot
T669	Phosphorylation		Uniprot
S671	Phosphorylation		Uniprot
S672	Phosphorylation		Uniprot
K673	Ubiquitination		Uniprot
S676	Phosphorylation	Q13315 (ATM)	Uniprot
S678	Phosphorylation	Q13315 (ATM)	Uniprot
S681	Phosphorylation		Uniprot
S688	Phosphorylation		Uniprot
S689	Phosphorylation		Uniprot
S701	Phosphorylation		Uniprot
S702	Phosphorylation		Uniprot

Research Backgrounds

P49959_MRE11_HUMAN

Function:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

PTMs:

Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.

Subcellular Location:

Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.

Subunit Structure:

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Interacts with ATF2. Interacts with EXD2. Interacts with MRNIP. Interacts with SAMHD1; leading to stimulate 3'-5' exonuclease activity. Interacts (when ubiquitinated) with UBQLN4 (via its UBA domain).

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

Family&Domains:

Belongs to the MRE11/RAD32 family.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

Restrictive clause

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For Research Use Only.
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Phospho-Mre11 (Ser676) Antibody - #AF8445