Phospho-LTK/ALK (Tyr672/Tyr1278) Antibody - #AF8442
| Product: | Phospho-LTK/ALK (Tyr672/Tyr1278) Antibody |
| Catalog: | AF8442 |
| Description: | Rabbit polyclonal antibody to Phospho-LTK/ALK (Tyr672/Tyr1278) |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Horse, Rabbit, Chicken |
| Mol.Wt.: | 176KD; 92kD,176kD(Calculated). |
| Uniprot: | P29376 | Q9UM73 |
| RRID: | AB_2840499 |
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8442, RRID:AB_2840499.
Fold/Unfold
Leukocyte receptor tyrosine kinase; Leukocyte tyrosine kinase; Leukocyte tyrosine kinase receptor; Ltk; LTK_HUMAN; Protein tyrosine kinase 1; TYK 1; TYK1; Alk; ALK tyrosine kinase receptor; ALK/EML4 fusion gene, included; ALK/NPM1 fusion gene, included; ALK_HUMAN; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; anaplastic lymphoma receptor tyrosine kinase; CD 246; CD246; CD246 antigen; EC 2.7.10.1; Ki 1; Ki1; mutant anaplastic lymphoma kinase; NBLST 3; NBLST3; Tcrz; TFG/ALK;
Immunogens
Expressed in non-hematopoietic cell lines and T- and B-cell lines.
Q9UM73 ALK_HUMAN:Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
- P29376 LTK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGCWGQLLVWFGAAGAILCSSPGSQETFLRSSPLPLASPSPRDPKVSAPPSILEPASPLNSPGTEGSWLFSTCGASGRHGPTQTQCDGAYAGTSVVVTVGAAGQLRGVQLWRVPGPGQYLISAYGAAGGKGAKNHLSRAHGVFVSAIFSLGLGESLYILVGQQGEDACPGGSPESQLVCLGESRAVEEHAAMDGSEGVPGSRRWAGGGGGGGGATYVFRVRAGELEPLLVAAGGGGRAYLRPRDRGRTQASPEKLENRSEAPGSGGRGGAAGGGGGWTSRAPSPQAGRSLQEGAEGGQGCSEAWATLGWAAAGGFGGGGGACTAGGGGGGYRGGDASETDNLWADGEDGVSFIHPSSELFLQPLAVTENHGEVEIRRHLNCSHCPLRDCQWQAELQLAECLCPEGMELAVDNVTCMDLHKPPGPLVLMVAVVATSTLSLLMVCGVLILVKQKKWQGLQEMRLPSPELELSKLRTSAIRTAPNPYYCQVGLGPAQSWPLPPGVTEVSPANVTLLRALGHGAFGEVYEGLVIGLPGDSSPLQVAIKTLPELCSPQDELDFLMEALIISKFRHQNIVRCVGLSLRATPRLILLELMSGGDMKSFLRHSRPHLGQPSPLVMRDLLQLAQDIAQGCHYLEENHFIHRDIAARNCLLSCAGPSRVAKIGDFGMARDIYRASYYRRGDRALLPVKWMPPEAFLEGIFTSKTDSWSFGVLLWEIFSLGYMPYPGRTNQEVLDFVVGGGRMDPPRGCPGPVYRIMTQCWQHEPELRPSFASILERLQYCTQDPDVLNSLLPMELGPTPEEEGTSGLGNRSLECLRPPQPQELSPEKLKSWGGSPLGPWLSSGLKPLKSRGLQPQNLWNPTYRS
- Q9UM73 ALK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGAIGLLWLLPLLLSTAAVGSGMGTGQRAGSPAAGPPLQPREPLSYSRLQRKSLAVDFVVPSLFRVYARDLLLPPSSSELKAGRPEARGSLALDCAPLLRLLGPAPGVSWTAGSPAPAEARTLSRVLKGGSVRKLRRAKQLVLELGEEAILEGCVGPPGEAAVGLLQFNLSELFSWWIRQGEGRLRIRLMPEKKASEVGREGRLSAAIRASQPRLLFQIFGTGHSSLESPTNMPSPSPDYFTWNLTWIMKDSFPFLSHRSRYGLECSFDFPCELEYSPPLHDLRNQSWSWRRIPSEEASQMDLLDGPGAERSKEMPRGSFLLLNTSADSKHTILSPWMRSSSEHCTLAVSVHRHLQPSGRYIAQLLPHNEAAREILLMPTPGKHGWTVLQGRIGRPDNPFRVALEYISSGNRSLSAVDFFALKNCSEGTSPGSKMALQSSFTCWNGTVLQLGQACDFHQDCAQGEDESQMCRKLPVGFYCNFEDGFCGWTQGTLSPHTPQWQVRTLKDARFQDHQDHALLLSTTDVPASESATVTSATFPAPIKSSPCELRMSWLIRGVLRGNVSLVLVENKTGKEQGRMVWHVAAYEGLSLWQWMVLPLLDVSDRFWLQMVAWWGQGSRAIVAFDNISISLDCYLTISGEDKILQNTAPKSRNLFERNPNKELKPGENSPRQTPIFDPTVHWLFTTCGASGPHGPTQAQCNNAYQNSNLSVEVGSEGPLKGIQIWKVPATDTYSISGYGAAGGKGGKNTMMRSHGVSVLGIFNLEKDDMLYILVGQQGEDACPSTNQLIQKVCIGENNVIEEEIRVNRSVHEWAGGGGGGGGATYVFKMKDGVPVPLIIAAGGGGRAYGAKTDTFHPERLENNSSVLGLNGNSGAAGGGGGWNDNTSLLWAGKSLQEGATGGHSCPQAMKKWGWETRGGFGGGGGGCSSGGGGGGYIGGNAASNNDPEMDGEDGVSFISPLGILYTPALKVMEGHGEVNIKHYLNCSHCEVDECHMDPESHKVICFCDHGTVLAEDGVSCIVSPTPEPHLPLSLILSVVTSALVAALVLAFSGIMIVYRRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEPSSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTILKSKNSMNQPGP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P29376/Q9UM73 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y46 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| Y705 | Phosphorylation | Uniprot | |
| S1075 | Phosphorylation | Uniprot | |
| Y1078 | Phosphorylation | Uniprot | |
| T1090 | Phosphorylation | Uniprot | |
| Y1092 | Phosphorylation | Uniprot | |
| Y1096 | Phosphorylation | Uniprot | |
| Y1131 | Phosphorylation | Uniprot | |
| Y1278 | Phosphorylation | Q9UM73 (ALK) | Uniprot |
| Y1282 | Phosphorylation | Q9UM73 (ALK) | Uniprot |
| Y1283 | Phosphorylation | Q9UM73 (ALK) | Uniprot |
| Y1359 | Phosphorylation | Uniprot | |
| Y1401 | Phosphorylation | Uniprot | |
| K1451 | Methylation | Uniprot | |
| K1455 | Methylation | Uniprot | |
| S1481 | Phosphorylation | Uniprot | |
| S1495 | Phosphorylation | Uniprot | |
| T1506 | Phosphorylation | Uniprot | |
| Y1507 | Phosphorylation | Uniprot | |
| S1509 | Phosphorylation | Uniprot | |
| K1517 | Ubiquitination | Uniprot | |
| K1518 | Ubiquitination | Uniprot | |
| Y1584 | Phosphorylation | Uniprot | |
| Y1586 | Phosphorylation | Uniprot | |
| T1597 | Phosphorylation | Uniprot | |
| Y1604 | Phosphorylation | Q9UM73 (ALK) | Uniprot |
| K1610 | Methylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S259 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| Y672 | Phosphorylation | Uniprot | |
| Y676 | Phosphorylation | Uniprot | |
| Y677 | Phosphorylation | Uniprot |
PTMs - P29376/Q9UM73 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| P14618 (PKM) | Y105 | Uniprot |
| P23246 (SFPQ) | Y293 | Uniprot |
| P31939 (ATIC) | Y104 | Uniprot |
| P40763 (STAT3) | Y705 | Uniprot |
| P42768 (WAS) | Y102 | Uniprot |
| P49841 (GSK3B) | S9 | Uniprot |
| P50552 (VASP) | Y39 | Uniprot |
| P62993 (GRB2) | Y160 | Uniprot |
| Q06124 (PTPN11) | Y546 | Uniprot |
| Q06124 (PTPN11) | Y584 | Uniprot |
| Q86WB0 (ZC3HC1) | Y105 | Uniprot |
| Q86WB0 (ZC3HC1) | Y137 | Uniprot |
| Q9C0H9-5 (SRCIN1) | Y396 | Uniprot |
| Q9UM73 (ALK) | Y1278 | Uniprot |
| Q9UM73 (ALK) | Y1282 | Uniprot |
| Q9UM73 (ALK) | Y1283 | Uniprot |
| Q9UM73 (ALK) | Y1604 | Uniprot |
| Substrate | Site | Source |
|---|
Research Backgrounds
Receptor with a tyrosine-protein kinase activity. The exact function of this protein is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport.
Membrane>Single-pass type I membrane protein.
Expressed in non-hematopoietic cell lines and T- and B-cell lines.
Homodimer when bound to ligand (Probable). Part of a complex including LTK, TNK2 and GRB2, in which GRB2 promotes LTK recruitment by TNK2.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.
N-glycosylated.
Cell membrane>Single-pass type I membrane protein.
Note: Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.
Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
Homodimer. Homodimerizes when bound to ligand. Interacts with FRS2, IRS1, MDK, PTN and SHC1. Interacts with CBL, PIK3R1 and PLCG1 (By similarity).
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Research Fields
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
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