Phospho-EPHB4 (Tyr987) Antibody - #AF8432

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Product: Phospho-EPHB4 (Tyr987) Antibody
Catalog: AF8432
Description: Rabbit polyclonal antibody to Phospho-EPHB4 (Tyr987)
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 108 kDa; 108kD(Calculated).
Uniprot: P54760
RRID: AB_2840489

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(86%), Bovine(86%), Horse(86%), Sheep(83%), Rabbit(86%), Dog(86%)
Clonality:
Polyclonal
Specificity:
Phospho-EPHB4 (Tyr987) Antibody detects endogenous levels of EPHB4 only when phosphorylated at Tyr987.
RRID:
AB_2840489
Cite Format: Affinity Biosciences Cat# AF8432, RRID:AB_2840489.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ephb4; EPHB4_HUMAN; Ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma Transmembrane Kinase; HTK; MYK 1; MYK1; TYRO 11; TYRO11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11;

Immunogens

Immunogen:
Uniprot:

P54760(EPHB4_HUMAN) >>Visit HPA database.

Gene(ID):
EPHB4(2050)
Expression:
P54760 EPHB4_HUMAN:

Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.

Sequence:
MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Horse
86
Bovine
86
Dog
86
Rabbit
86
Sheep
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54760 As Substrate

Site PTM Type Enzyme
T34 Phosphorylation
K196 Acetylation
T321 Phosphorylation
T322 Phosphorylation
S332 Phosphorylation
S337 Phosphorylation
Y357 Phosphorylation
K474 Ubiquitination
T544 Phosphorylation
Y574 Phosphorylation
S575 Phosphorylation
K577 Ubiquitination
Y581 Phosphorylation
T587 Phosphorylation
Y590 Phosphorylation
T595 Phosphorylation
Y596 Phosphorylation
K608 Ubiquitination
Y614 Phosphorylation
K640 Ubiquitination
K647 Ubiquitination
K650 Ubiquitination
T713 Phosphorylation
S750 Phosphorylation
S757 Phosphorylation
S762 Phosphorylation
S769 Phosphorylation
S770 Phosphorylation
T773 Phosphorylation
Y774 Phosphorylation
T775 Phosphorylation
S776 Phosphorylation
S777 Phosphorylation
K781 Ubiquitination
S872 Phosphorylation
K885 Ubiquitination
Y906 Phosphorylation
S911 Phosphorylation
Y924 Phosphorylation
S943 Phosphorylation
T953 Phosphorylation
K960 Ubiquitination
S964 Phosphorylation
K973 Ubiquitination
T976 Phosphorylation
Y987 Phosphorylation

PTMs - P54760 As Enzyme

Substrate Site Source
O75056 (SDC3) Y409 Uniprot
O75056 (SDC3) Y419 Uniprot
O75056 (SDC3) Y431 Uniprot

Research Backgrounds

Function:

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.

PTMs:

Phosphorylated; autophosphorylation is stimulated by EFNB2.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.

Subunit Structure:

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts with RASA1; the interaction depends on EPHB4 tyrosine-phosphorylation.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). The protective role of Ephrin-B2/EphB4 signaling in osteogenic differentiation under inflammatory environment. Experimental Cell Research, 2021 (PubMed: 33516666) [IF=3.7]

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