Phospho-EPHA1 (Tyr781) Antibody | Affinity Biosciences

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Product:	Phospho-EPHA1 (Tyr781) Antibody
Catalog:	AF8423
Description:	Rabbit polyclonal antibody to Phospho-EPHA1 (Tyr781)
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	108 kDa; 108kD(Calculated).
Uniprot:	P21709
RRID:	AB_2840480

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

Phospho-EPHA1 (Tyr781) Antibody detects endogenous levels of EPHA1 only when phosphorylated at Tyr781.

RRID:

AB_2840480
Cite Format: Affinity Biosciences Cat# AF8423, RRID:AB_2840480.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EPH; EPH receptor A1; EPH tyrosine kinase 1; EPH tyrosine kinase; Epha1; EPHA1_HUMAN; Ephrin receptor Eph A1; Ephrin type A receptor 1; Ephrin type-A receptor 1; EPHT 1; EPHT; EPHT1; Erythropoietin producing hepatoma amplified sequence; Erythropoietin-producing hepatoma receptor; Esk; hEpha1; MGC163163; Oncogene EPH; Soluble EPHA1 variant 1; Soluble EPHA1 variant 2; Tyrosine protein kinase receptor EPH; Tyrosine-protein kinase receptor EPH;

Immunogens

Immunogen:

Uniprot:

P21709(EPHA1_HUMAN) >>Visit HPA database.

Gene(ID):

EPHA1(2041)

Expression:

P21709 EPHA1_HUMAN:

Overexpressed in several carcinomas.

Sequence:

P21709 EPHA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MERRWPLGLGLVLLLCAPLPPGARAKEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNGTPLYMYQDCPMQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLVSGSVKLNVERCSLGRLTRRGLYLAFHNPGACVALVSVRVFYQRCPETLNGLAQFPDTLPGPAGLVEVAGTCLPHARASPRPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVACPSGSYRMDMDTPHCLTCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSLRWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLEPYANYTFNVEAQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLTYELHVLNQDEERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGEIVAVIFGLLLGAALLLGILVFRSRRAQRQRQQRQRDRATDVDREDKLWLKPYVDLQAYEDPAQGALDFTRELDPAWLMVDTVIGEGEFGEVYRGTLRLPSQDCKTVAIKTLKDTSPGGQWWNFLREATIMGQFSHPHILHLEGVVTKRKPIMIITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGLTRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIEDGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHLEQLLANPHSLRTIANFDPRMTLRLPSLSGSDGIPYRTVSEWLESIRMKRYILHFHSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Dog

Rabbit

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21709 As Substrate

P21709

Site	PTM Type	Source
T33	Phosphorylation	Uniprot
S34	Phosphorylation	Uniprot
S153	Phosphorylation	Uniprot
T155	Phosphorylation	Uniprot
K593	Ubiquitination	Uniprot
K597	Ubiquitination	Uniprot
Y599	Phosphorylation	Uniprot
Y605	Phosphorylation	Uniprot
T780	Phosphorylation	Uniprot
Y781	Phosphorylation	Uniprot
T783	Phosphorylation	Uniprot
K787	Ubiquitination	Uniprot
T805	Phosphorylation	Uniprot
S811	Phosphorylation	Uniprot
T892	Phosphorylation	Uniprot
S906	Phosphorylation	Uniprot
S908	Phosphorylation	Uniprot
S910	Phosphorylation	Uniprot
S919	Phosphorylation	Uniprot

Research Backgrounds

P21709_EPHA1_HUMAN

Function:

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.

PTMs:

Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.

Ubiquitinated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Overexpressed in several carcinomas.

Subunit Structure:

Homodimer. Forms a signaling complex with LCK; PTK2B/PYK2 and PI3-kinase upon activation by EFNA1; regulates T-lymphocytes migration. Interacts (via SAM domain) with ILK (via ANK repeats); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts (kinase activity-dependent) with PTK2/FAK1.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance. (View pathway)

Restrictive clause

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For Research Use Only.
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Phospho-EPHA1 (Tyr781) Antibody - #AF8423