Phospho-RBL2 (Ser639) Antibody - #AF8401

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Product: Phospho-RBL2 (Ser639) Antibody
Catalog: AF8401
Description: Rabbit polyclonal antibody to Phospho-RBL2 (Ser639)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 128kDa; 128kD(Calculated).
Uniprot: Q08999
RRID: AB_2840462

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(89%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-RBL2 (Ser639) Antibody detects endogenous levels of RBL2 only when phosphorylated at Ser639.
RRID:
AB_2840462
Cite Format: Affinity Biosciences Cat# AF8401, RRID:AB_2840462.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

130 kDa retinoblastoma associated protein; 130 kDa retinoblastoma-associated protein; IB4; p130; PPAR-alpha-interacting complex protein 128; PRB2; Rb2; Rbl2; RBL2_HUMAN; RBR 2; RBR-2; RBR2; Retinoblastoma like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2; RIC128;

Immunogens

Immunogen:
Uniprot:

Q08999(RBL2_HUMAN) >>Visit HPA database.

Gene(ID):
RBL2(5934)
Sequence:
MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDEAARAEAWDSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTVSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLCSLHDGLVLEAKGIKEHFWKPYIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDERIFLGEDAEEEIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVSTATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNENRVPTCEEVMPPQNLERADEICIAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFFRKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQPSSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYPFVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGSH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
89
Zebrafish
71
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08999 As Substrate

Site PTM Type Enzyme
S98 Phosphorylation
Y111 Phosphorylation
S113 Phosphorylation
T115 Phosphorylation
K255 Ubiquitination
K314 Ubiquitination
T364 Phosphorylation
S366 Phosphorylation
S373 Phosphorylation
K384 Ubiquitination
K393 Ubiquitination
T401 Phosphorylation P11802 (CDK4) , Q00534 (CDK6)
T404 Phosphorylation
S413 Phosphorylation P24941 (CDK2)
T417 Phosphorylation P24941 (CDK2)
K443 Ubiquitination
S531 Phosphorylation
S543 Phosphorylation
Y544 Phosphorylation
S603 Phosphorylation
S639 Phosphorylation P24941 (CDK2)
T642 Phosphorylation P24941 (CDK2)
T647 Phosphorylation
S659 Phosphorylation Q9HC98 (NEK6)
T661 Phosphorylation
S662 Phosphorylation P24941 (CDK2)
T664 Phosphorylation
T665 Phosphorylation
Y667 Phosphorylation
Y670 Phosphorylation
S672 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
S676 Phosphorylation
T678 Phosphorylation
S688 Phosphorylation P24941 (CDK2)
S690 Phosphorylation
T694 Phosphorylation P24941 (CDK2)
S941 Phosphorylation
S948 Phosphorylation
S952 Phosphorylation P24941 (CDK2)
T954 Phosphorylation
T961 Phosphorylation
S962 Phosphorylation
S966 Phosphorylation
S971 Phosphorylation
S972 Phosphorylation
S973 Phosphorylation
T974 Phosphorylation
S981 Phosphorylation
S982 Phosphorylation
T986 Phosphorylation
T988 Phosphorylation
S995 Phosphorylation
T1019 Phosphorylation
S1035 Phosphorylation P11802 (CDK4) , Q00534 (CDK6)
Y1037 Phosphorylation
T1042 Phosphorylation
S1044 Phosphorylation P24941 (CDK2)
Y1057 Phosphorylation
S1059 Phosphorylation
T1065 Phosphorylation
S1068 Phosphorylation P24941 (CDK2)
S1078 Phosphorylation
S1080 Phosphorylation P24941 (CDK2)
S1082 Phosphorylation
S1090 Phosphorylation
T1097 Phosphorylation P24941 (CDK2)
S1112 Phosphorylation P24941 (CDK2)
S1138 Phosphorylation

Research Backgrounds

Function:

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

PTMs:

During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with AATF. Interacts with KMT5B, KMT5C and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RINT1. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5). Interacts with RBBP9 (By similarity).

Family&Domains:

Belongs to the retinoblastoma protein (RB) family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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