Phospho-IRF5 (Ser437) Antibody | Affinity Biosciences

IHC
IF/ICC
pElisa
Cites

1/4

AF8382 at 1/100 staining Rat thymus tissue by IHC-P.

AF8382 staining Hela cells(4h of LPS treatment) by IF/ICC.

peptide-ELISA analysis of AF8382.

FIGURE 4 Cytoplasmic mtDNA markedly activated STING signalling.

Product:	Phospho-IRF5 (Ser437) Antibody
Catalog:	AF8382
Description:	Rabbit polyclonal antibody to Phospho-IRF5 (Ser437)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	56kDa; 56kD(Calculated).
Uniprot:	Q13568
RRID:	AB_2840443

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-IRF5 (Ser437) Antibody detects endogenous levels of IRF5 only when phosphorylated at Ser437.

RRID:

AB_2840443
Cite Format: Affinity Biosciences Cat# AF8382, RRID:AB_2840443.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Interferon regulatory factor 5; Interferon regulatory factor 5 bone marrow variant; IRF 5; IRF-5; Irf5; IRF5_HUMAN; SLEB10;

Immunogens

Immunogen:

Uniprot:

Q13568(IRF5_HUMAN) >>Visit HPA database.

Gene(ID):

IRF5(3663)

Sequence:

Q13568 IRF5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13568 As Substrate

Q13568

Site	PTM Type	Enzyme	Source
T10	Phosphorylation		Uniprot
Y136	Phosphorylation		Uniprot
S158	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
S293	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
S301	Phosphorylation		Uniprot
K309	Ubiquitination		Uniprot
Y313	Phosphorylation		Uniprot
Y335	Phosphorylation		Uniprot
K411	Ubiquitination		Uniprot
K412	Ubiquitination		Uniprot
S435	Phosphorylation	O43353 (RIPK2)	Uniprot
S437	Phosphorylation		Uniprot
S440	Phosphorylation		Uniprot
S446	Phosphorylation	O14920 (IKBKB) , O43353 (RIPK2)	Uniprot

Research Backgrounds

Q13568_IRF5_HUMAN

Function:

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.

PTMs:

Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.

'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the cytoplasm.

Subunit Structure:

Homodimer, when phosphorylated.

Family&Domains:

Belongs to the IRF family.

Research Fields

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

References

1). Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling. CELL PROLIFERATION, 2022 (PubMed: 36106559) [IF=8.5]

Application: WB Species: Mice Sample: BMDCs

FIGURE 4 Cytoplasmic mtDNA markedly activated STING signalling. BMDCs were treated with mtDNA (10 μg/ml), or transfected with vehicle or mtDNA (10 μg/ml) for 24 h, then were harvested for western blot and qPCR. (A) Representative blots and relative quantification protein levels of STING pathway (n = 3). (B) qPCR quantitation of IFN‐β (n = 7). (C) Representative images of nuclear translocation of IRF3 in BMDC treated as indicated. Scale bar: 5 μm. (D) Representative blots and relative quantification protein levels of TLR9 pathway (n = 3). (E) qPCR quantitation of TNF‐α, IL‐6 and VCAM‐1 (n = 6–7). (F) Representative blots and relative quantification protein levels of NLRP3 pathway (n = 3). (G) qPCR quantitation of IL‐1β, IL‐18 (n = 7). Data are shown as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. BMDC, bone marrow derived dendritic cell; IFN, interferon; mtDNA, mitochondrial DNA; mtDNA (T), mtDNA transfection; Veh (T), vehicle transfection.

2). miR-31-5p Regulates Type I Interferon by Targeting SLC15A4 in Plasmacytoid Dendritic Cells of Systemic Lupus Erythematosus. Journal of Inflammation Research, 2022 (PubMed: 36510495) [IF=4.5]

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Phospho-IRF5 (Ser437) Antibody - #AF8382