Phospho-HtrA2 (Ser142) Antibody | Affinity Biosciences

IHC
pElisa

1/2

AF8381 at 1/100 staining Mouse colon tissue by IHC-P.

Product:	Phospho-HtrA2 (Ser142) Antibody
Catalog:	AF8381
Description:	Rabbit polyclonal antibody to Phospho-HtrA2 (Ser142)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	O43464
RRID:	AB_2840442

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(89%), Dog(89%)

Clonality:

Polyclonal

Specificity:

Phospho-HtrA2 (Ser142) Antibody detects endogenous levels of HtrA2 only when phosphorylated at Ser142.

RRID:

AB_2840442
Cite Format: Affinity Biosciences Cat# AF8381, RRID:AB_2840442.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA, E. coli, homolog of, 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Protease serine 25; PRSS 25; PRSS25; Serine protease 25; Serine protease HTRA2; Serine protease HTRA2 mitochondrial; Serine protease htra2 mitochondrial precursor; Serine protease omi; Serine proteinase OMI;

Immunogens

Immunogen:

Uniprot:

O43464(HTRA2_HUMAN) >>Visit HPA database.

Gene(ID):

HTRA2(27429)

Expression:

O43464 HTRA2_HUMAN:

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Sequence:

O43464 HTRA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTSDPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43464 As Substrate

O43464

Site	PTM Type	Enzyme	Source
R5	Methylation		Uniprot
R8	Methylation		Uniprot
T51	Phosphorylation		Uniprot
T54	Phosphorylation		Uniprot
S96	Phosphorylation		Uniprot
S142	Phosphorylation	Q9BXM7 (PINK1) , P53778 (MAPK12)	Uniprot
T157	Phosphorylation		Uniprot
S212	Phosphorylation	P31751 (AKT2) , P31749 (AKT1)	Uniprot
K237	Ubiquitination		Uniprot
T242	Phosphorylation		Uniprot
T326	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
S352	Phosphorylation		Uniprot
K395	Ubiquitination		Uniprot
S400	Phosphorylation	Q00535 (CDK5)	Uniprot
T453	Phosphorylation		Uniprot

Research Backgrounds

O43464_HTRA2_HUMAN

Function:

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.

PTMs:

Autoproteolytically activated.

Subcellular Location:

Mitochondrion intermembrane space. Mitochondrion membrane>Single-pass membrane protein.
Note: Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.

Tissue Specificity:

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Subunit Structure:

Homotrimer. Interacts with MXI2. Interacts with THAP5 under apoptotic conditions. The mature protein, but not the precursor, binds to BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts with BIRC6/bruce.

Family&Domains:

The mature N-terminus is involved in the interaction with XIAP.

The PDZ domain mediates interaction with MXI2.

Belongs to the peptidase S1C family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-HtrA2 (Ser142) Antibody - #AF8381