Phospho-HNRNPA0 (Ser84) Antibody - #AF8379
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| Product: | Phospho-HNRNPA0 (Ser84) Antibody |
| Catalog: | AF8379 |
| Description: | Rabbit polyclonal antibody to Phospho-HNRNPA0 (Ser84) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 31kDa; 31kD(Calculated). |
| Uniprot: | Q13151 |
| RRID: | AB_2840440 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-HNRNPA0 (Ser84) Antibody detects endogenous levels of HNRNPA0 only when phosphorylated at Ser84.
RRID:
AB_2840440
Cite Format: Affinity Biosciences Cat# AF8379, RRID:AB_2840440.
Cite Format: Affinity Biosciences Cat# AF8379, RRID:AB_2840440.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Heterogeneous nuclear ribonucleoprotein A0; hnRNA binding protein; hnRNP A0; HNRNPA0; HNRPA0; ROA0_HUMAN;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q13151 ROA0_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENSQLCKLFIGGLNVQTSESGLRGHFEAFGTLTDCVVVVNPQTKRSRCFGFVTYSNVEEADAAMAASPHAVDGNTVELKRAVSREDSARPGAHAKVKKLFVGGLKGDVAEGDLIEHFSQFGTVEKAEIIADKQSGKKRGFGFVYFQNHDAADKAAVVKFHPIQGHRVEVKKAVPKEDIYSGGGGGGSRSSRGGRGGRGRGGGRDQNGLSKGGGGGYNSYGGYGGGGGGGYNAYGGGGGGSSYGGSDYGNGFGGFGSYSQHQSSYGPMKSGGGGGGGGSSWGGRSNSGPYRGGYGGGGGYGGSSF
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Pig
0
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13151 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S84 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| S88 | Phosphorylation | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Sumoylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K106 | Sumoylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K126 | Sumoylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K133 | Acetylation | Uniprot | |
| K133 | Sumoylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| K138 | Sumoylation | Uniprot | |
| R139 | Methylation | Uniprot | |
| Y145 | Phosphorylation | Uniprot | |
| K154 | Sumoylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K159 | Acetylation | Uniprot | |
| K159 | Sumoylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K171 | Sumoylation | Uniprot | |
| K172 | Sumoylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K176 | Sumoylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| R189 | Methylation | Uniprot | |
| R192 | Methylation | Uniprot | |
| R195 | Methylation | Uniprot | |
| R198 | Methylation | Uniprot | |
| R200 | Methylation | Uniprot | |
| R204 | Methylation | Uniprot | |
| K211 | Sumoylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| K269 | Sumoylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| R284 | Methylation | Uniprot | |
| R291 | Methylation | Uniprot | |
| S303 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
mRNA-binding component of ribonucleosomes. Specifically binds AU-rich element (ARE)-containing mRNAs. Involved in post-transcriptional regulation of cytokines mRNAs.
PTMs:
Phosphorylated at Ser-84 by MAPKAPK2 in response to LPS treatment, promoting stabilization of GADD45A mRNA.
Arg-291 is dimethylated, probably to asymmetric dimethylarginine.
Subcellular Location:
Nucleus.
Note: Component of ribonucleosomes.
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