Phospho-GRK1/2 (Tyr13) Antibody - #AF8376

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Product: Phospho-GRK1/2 (Tyr13) Antibody
Catalog: AF8376
Description: Rabbit polyclonal antibody to Phospho-GRK1/2 (Tyr13)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Dog, Chicken
Mol.Wt.: 79KD; 80kD(Calculated).
Uniprot: P25098 | P35626
RRID: AB_2840437

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-GRK1/2 (Tyr13) Antibody detects endogenous levels of GRK1/2 only when phosphorylated at Tyr13.
RRID:
AB_2840437
Cite Format: Affinity Biosciences Cat# AF8376, RRID:AB_2840437.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADRBK1; Adrenergic beta receptor kinase 1; ARBK1_HUMAN; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; FLJ16718; G protein coupled receptor kinase 2; G-protein coupled receptor kinase 2; GRK2; ADRBK2; Adrenergic, beta, receptor kinase 2; ARBK2_HUMAN; BARK2; Beta adrenergic receptor kinase 2; Beta ARK 2; Beta-adrenergic receptor kinase 2; Beta-ARK-2; EC 2.7.11.15; G protein coupled receptor kinase 3; G-protein-coupled receptor kinase 3; GRK3;

Immunogens

Immunogen:
Uniprot:

P25098(ARBK1_HUMAN) >>Visit HPA database.

P35626(ARBK2_HUMAN) >>Visit HPA database.

Gene(ID):
GRK2(156) | GRK3(157)
Expression:
P25098 ARBK1_HUMAN:

Expressed in peripheral blood leukocytes.

Sequence:
MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFNQKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPELKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Chicken
100
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25098/P35626 As Substrate

Site PTM Type Enzyme
K45 Ubiquitination
K57 Ubiquitination
K62 Ubiquitination
K84 Ubiquitination
K90 Ubiquitination
K94 Ubiquitination
Y109 Phosphorylation
Y112 Phosphorylation
K115 Ubiquitination
K126 Ubiquitination
K138 Ubiquitination
K139 Ubiquitination
K164 Ubiquitination
K170 Ubiquitination
Y206 Phosphorylation
T213 Phosphorylation
K215 Ubiquitination
K220 Ubiquitination
K230 Ubiquitination
K319 Ubiquitination
K344 Ubiquitination
K345 Ubiquitination
K346 Ubiquitination
S350 Phosphorylation
T353 Phosphorylation
Y356 Phosphorylation
S423 Phosphorylation
K448 Ubiquitination
K454 Ubiquitination
K465 Ubiquitination
S487 Phosphorylation
K494 Ubiquitination
K497 Ubiquitination
K507 Ubiquitination
S514 Phosphorylation
T524 Phosphorylation
Y526 Phosphorylation
K545 Ubiquitination
Y564 Phosphorylation
T612 Phosphorylation
K625 Acetylation
K628 Acetylation
K628 Ubiquitination
K644 Ubiquitination
K645 Ubiquitination
K652 Ubiquitination
S670 Phosphorylation
T672 Phosphorylation
S685 Phosphorylation
Site PTM Type Enzyme
Y13 Phosphorylation P00533 (EGFR) , P12931 (SRC)
S29 Phosphorylation P17252 (PRKCA) , P05129 (PRKCG) , Q05655 (PRKCD)
K31 Ubiquitination
K45 Ubiquitination
R50 Methylation
T54 Phosphorylation
K57 Ubiquitination
K62 Ubiquitination
Y86 Phosphorylation P00533 (EGFR) , P12931 (SRC)
Y92 Phosphorylation P12931 (SRC) , P00533 (EGFR)
K94 Ubiquitination
K115 Ubiquitination
K126 Ubiquitination
K138 Ubiquitination
K139 Ubiquitination
K164 Ubiquitination
K170 Ubiquitination
Y206 Phosphorylation
T213 Phosphorylation
K215 Ubiquitination
K220 Ubiquitination
K230 Ubiquitination
S247 Phosphorylation
T248 Phosphorylation
T263 Phosphorylation
K319 Ubiquitination
K344 Ubiquitination
K345 Ubiquitination
K346 Ubiquitination
S350 Phosphorylation
T353 Phosphorylation
Y356 Phosphorylation
S416 Phosphorylation
S423 Phosphorylation
K448 Ubiquitination
S487 Phosphorylation
K494 Ubiquitination
K497 Ubiquitination
S501 Phosphorylation
Y506 Phosphorylation
K545 Ubiquitination
K557 Ubiquitination
K628 Ubiquitination
K644 Acetylation
K644 Ubiquitination
S670 Phosphorylation P27361 (MAPK3) , P24941 (CDK2)
S676 Phosphorylation
K677 Ubiquitination
R683 Methylation
S685 Phosphorylation P17612 (PRKACA)

PTMs - P25098/P35626 As Enzyme

Substrate Site Source
P07550 (ADRB2) S355 Uniprot
P07550 (ADRB2) S356 Uniprot
P21731-2 (TBXA2R) S239 Uniprot
P21731-2 (TBXA2R) S357 Uniprot
P30411 (BDKRB2) S366 Uniprot
P30411 (BDKRB2) S373 Uniprot
P30411 (BDKRB2) S375 Uniprot
P30874 (SSTR2) S341 Uniprot
P30874 (SSTR2) S343 Uniprot
P35372 (OPRM1) T182 Uniprot
P41146 (OPRL1) S363 Uniprot
P51681 (CCR5) S336 Uniprot
P51681 (CCR5) S337 Uniprot
P51681 (CCR5) S342 Uniprot
P51681 (CCR5) S349 Uniprot
Substrate Site Source
P05387 (RPLP2) S102 Uniprot
P05387 (RPLP2) S105 Uniprot
P07550 (ADRB2) S355 Uniprot
P07550 (ADRB2) S356 Uniprot
P07550 (ADRB2) T384 Uniprot
P07550 (ADRB2) S396 Uniprot
P07550 (ADRB2) S401 Uniprot
P07550 (ADRB2) S407 Uniprot
P07550 (ADRB2) S411 Uniprot
P08069 (IGF1R) S1278 Uniprot
P08172 (CHRM2) S232 Uniprot
P08172 (CHRM2) S234 Uniprot
P08172 (CHRM2) S250 Uniprot
P08172 (CHRM2) S251 Uniprot
P08172 (CHRM2) T271 Uniprot
P08172 (CHRM2) S282 Uniprot
P08172 (CHRM2) S283 Uniprot
P08172 (CHRM2) S286 Uniprot
P08172 (CHRM2) T287 Uniprot
P08172 (CHRM2) S288 Uniprot
P08172 (CHRM2) S290 Uniprot
P08172 (CHRM2) S294 Uniprot
P08172 (CHRM2) T302 Uniprot
P08172 (CHRM2) T307 Uniprot
P08172 (CHRM2) S309 Uniprot
P08172 (CHRM2) T310 Uniprot
P08172 (CHRM2) S311 Uniprot
P09497 (CLTB) S205 Uniprot
P15311 (EZR) T567 Uniprot
P18545 (PDE6G) T62 Uniprot
P21731-2 (TBXA2R) S239 Uniprot
P21731-2 (TBXA2R) S357 Uniprot
P26038 (MSN) T558 Uniprot
P30411 (BDKRB2) S366 Uniprot
P30411 (BDKRB2) S373 Uniprot
P30411 (BDKRB2) S375 Uniprot
P32245 (MC4R) T312 Uniprot
P32245 (MC4R) S329 Uniprot
P32245 (MC4R) S330 Uniprot
P35241 (RDX) T564 Uniprot
P35367 (HRH1) S398 Uniprot
P35372 (OPRM1) T356 Uniprot
P35372 (OPRM1) S357 Uniprot
P35372 (OPRM1) S358 Uniprot
P35372 (OPRM1) S377 Uniprot
P37840 (SNCA) S129 Uniprot
P41143 (OPRD1) T358 Uniprot
P41143 (OPRD1) S363 Uniprot
Q14940 (SLC9A5) S702 Uniprot
Q14940 (SLC9A5) S709 Uniprot
Q14940 (SLC9A5) S711 Uniprot
Q14940 (SLC9A5) S712 Uniprot
Q14940 (SLC9A5) T714 Uniprot
Q15796 (SMAD2) T197 Uniprot
Q16539 (MAPK14) T123 Uniprot
Q96A54 (ADIPOR1) S7 Uniprot
Q96A54 (ADIPOR1) T24 Uniprot
Q96A54 (ADIPOR1) T53 Uniprot
Q9Y2W7 (KCNIP3) S95 Uniprot

Research Backgrounds

Function:

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).

Subcellular Location:

Cytoplasm. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in peripheral blood leukocytes.

Subunit Structure:

Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1. Interacts with LPAR1 and LPAR2. Interacts with RALA in response to LPAR1 activation. ADRBK1 and RALA mutually inhibit each other's binding to LPAR1. Interacts with ADRB2.

Family&Domains:

The PH domain binds anionic phospholipids and helps recruiting ADRBK1 from the cytoplasm to plasma membrane close to activated receptors. It mediates binding to G protein beta and gamma subunits, competing with G-alpha subunits and other G-betagamma effectors.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Function:

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

PTMs:

Ubiquitinated.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

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