Phospho-ASAP2 (Ser701) Antibody - #AF8367

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Product: Phospho-ASAP2 (Ser701) Antibody
Catalog: AF8367
Description: Rabbit polyclonal antibody to Phospho-ASAP2 (Ser701)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 112kDa; 112kD(Calculated).
Uniprot: O43150
RRID: AB_2840428

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-ASAP2 (Ser701) Antibody detects endogenous levels of ASAP2 only when phosphorylated at Ser701.
RRID:
AB_2840428
Cite Format: Affinity Biosciences Cat# AF8367, RRID:AB_2840428.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AMAP 2; AMAP2; ANK repeat and PH domain-containing protein 2; Arf-GAP with SH3 domain; ArfGAP with SH3 domain, ankyrin repeat and PH domain 2; ASAP2; ASAP2_HUMAN; centaurin, beta 3; CENTB3; DDEF 2; DDEF2; Development and differentiation enhancing factor 2; Development and differentiation-enhancing factor 2; FLJ42910; KIAA0400; PAG 3; PAG3; Pap alpha; PAP; Paxillin associated protein with ARFGAP activity 3; Paxillin-associated protein with ARF GAP activity 3; Pyk2 C terminus associated protein; Pyk2 C-terminus-associated protein; SHAG 1; SHAG1;

Immunogens

Immunogen:
Uniprot:

O43150(ASAP2_HUMAN) >>Visit HPA database.

Gene(ID):
ASAP2(8853)
Expression:
O43150 ASAP2_HUMAN:

Detected in heart, brain, placenta, kidney, monocytes and pancreas.

Sequence:
MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINSSGLAHVENEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVEQKEDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAKNIGNAGFNEIMECCLPAEDSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTRDIFGLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDESDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLARDAANLAKEKQRAFMPSILQNETYGALLSGSPPPAQPAAPSTTSAPPLPPRNVGKVQTASSANTLWKTNSVSVDGGSRQRSSSDPPAVHPPLPPLRVTSTNPLTPTPPPPVAKTPSVMEALSQPSKPAPPGISQIRPPPLPPQPPSRLPQKKPAPGADKSTPLTNKGQPRGPVDLSATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNCVADNPDELTFSEGDVIIVDGEEDQEWWIGHIDGDPGRKGAFPVSFVHFIAD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43150 As Substrate

Site PTM Type Enzyme
K81 Ubiquitination
Y201 Phosphorylation
K212 Ubiquitination
K242 Acetylation
K242 Ubiquitination
K274 Ubiquitination
K282 Ubiquitination
S285 Phosphorylation
Y293 Phosphorylation
S294 Phosphorylation
S311 Phosphorylation
K321 Acetylation
K325 Acetylation
S329 Phosphorylation
T336 Phosphorylation
S338 Phosphorylation
K405 Ubiquitination
K546 Ubiquitination
K555 Ubiquitination
T556 Phosphorylation
K662 Ubiquitination
Y689 Phosphorylation
S701 Phosphorylation
S712 Phosphorylation
S722 Phosphorylation
Y724 Phosphorylation
S728 Phosphorylation
Y763 Phosphorylation
S800 Phosphorylation
S809 Phosphorylation
S820 Phosphorylation
S822 Phosphorylation
T845 Phosphorylation
T900 Phosphorylation
T903 Phosphorylation

Research Backgrounds

Function:

Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.

PTMs:

Phosphorylated on tyrosine residues by SRC and PTK2B.

Subcellular Location:

Cytoplasm. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein. Cell membrane>Peripheral membrane protein.
Note: Colocalizes with F-actin and ARF6 in phagocytic cups.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in heart, brain, placenta, kidney, monocytes and pancreas.

Subunit Structure:

Binds PXN, ARF1, ARF5, ARF6, PTK2B and SRC.

Family&Domains:

The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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