Phospho-5 Lipoxygenase (Ser664) Antibody - #AF8359

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Product: Phospho-5 Lipoxygenase (Ser664) Antibody
Catalog: AF8359
Description: Rabbit polyclonal antibody to Phospho-5 Lipoxygenase (Ser664)
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 77kDa; 78kD(Calculated).
Uniprot: P09917
RRID: AB_2840420

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
Phospho-5 Lipoxygenase (Ser664) Antibody detects endogenous levels of 5 Lipoxygenase only when phosphorylated at Ser664.
RRID:
AB_2840420
Cite Format: Affinity Biosciences Cat# AF8359, RRID:AB_2840420.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5 LO; 5 LOX; 5-lipoxygenase; 5-LO; 5-LOX; 5LOX; 5LPG; ALOX 5; Alox5; Arachidonate 5 lipoxygenase; Arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; LOX5_HUMAN; MGC163204;

Immunogens

Immunogen:
Uniprot:

P09917(LOX5_HUMAN) >>Visit HPA database.

Gene(ID):
ALOX5(240)
Sequence:
MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Chicken
88
Pig
50
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09917 As Substrate

Site PTM Type Enzyme
Y21 Phosphorylation
Y23 Phosphorylation
Y43 Phosphorylation
Y54 Phosphorylation
Y75 Phosphorylation
T87 Phosphorylation
Y95 Phosphorylation
Y101 Phosphorylation
T105 Phosphorylation
Y182 Phosphorylation
S183 Phosphorylation
T250 Phosphorylation
T259 Phosphorylation
T260 Phosphorylation
S266 Phosphorylation
S272 Phosphorylation Q9UQM7 (CAMK2A) , P17612 (PRKACA) , P49137 (MAPKAPK2)
T445 Phosphorylation
Y446 Phosphorylation
S448 Phosphorylation
T486 Phosphorylation
S523 Phosphorylation
S524 Phosphorylation P17612 (PRKACA)
K528 Acetylation
S532 Phosphorylation
S664 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S671 Phosphorylation

Research Backgrounds

Function:

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

PTMs:

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

Subcellular Location:

Cytoplasm. Nucleus matrix. Nucleus membrane>Peripheral membrane protein.
Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity.

Family&Domains:

Belongs to the lipoxygenase family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

References

1). NDUFA4L2 in smooth muscle promotes vascular remodeling in hypoxic pulmonary arterial hypertension. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33340241) [IF=5.3]

Application: WB    Species: Human    Sample: pulmonary artery smooth muscle cells (PASMCs)

FIGURE 7 NDUFA4L2 regulated downstream p38-5-LO pathway may be an important mechanism for hypoxic pulmonary vascular remodelling. A, After interference with NDUFA4L2, Western blotting was used to detect the expression of p-ERK/ERK, p-p38/ p38, p-ERK/ERK in hypoxic HPASMCs; (B) After interference with NDUFA4L2, Western blotting was used to detect the expression of p-5-LO/5-LO in hypoxic HPASMCs. NC, negative control; Nor, normoxia; Hyp, hypoxia. *P < .05, **P < .01. n = 6. All of the values are denoted as mean ± SEM

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