Phospho-TNNI3 (Ser23+Ser24) Antibody - #AF8353
| Product: | Phospho-TNNI3 (Ser23+Ser24) Antibody |
| Catalog: | AF8353 |
| Description: | Rabbit polyclonal antibody to Phospho-TNNI3 (Ser23+Ser24) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 28kDa; 24kD(Calculated). |
| Uniprot: | P19429 |
| RRID: | AB_2840415 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8353, RRID:AB_2840415.
Fold/Unfold
cardiac muscle; Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; Tn1; Tni; TNN I3; TNNC 1; TNNC1; TNNI3; TNNI3_HUMAN; Troponin I; Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; Ttroponin I type 3 (cardiac);
Immunogens
- P19429 TNNI3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P19429 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Q13976 (PRKG1) , Q15139 (PRKD1) , P17612 (PRKACA) , Q05655 (PRKCD) , Q13131 (PRKAA1) , P17252 (PRKCA) | Uniprot |
| S24 | Phosphorylation | Q05655 (PRKCD) , P17612 (PRKACA) , P17252 (PRKCA) , Q15139 (PRKD1) , Q13976 (PRKG1) , Q13131 (PRKAA1) | Uniprot |
| Y26 | Phosphorylation | Uniprot | |
| Y29 | Phosphorylation | Uniprot | |
| T31 | Phosphorylation | Q13043 (STK4) | Uniprot |
| S39 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K40 | Methylation | Uniprot | |
| S42 | Phosphorylation | P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB) | Uniprot |
| S44 | Phosphorylation | P05771 (PRKCB) , P17252 (PRKCA) , Q02156 (PRKCE) , P05129 (PRKCG) | Uniprot |
| T51 | Phosphorylation | Q13043 (STK4) | Uniprot |
| K72 | Acetylation | Uniprot | |
| S77 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T78 | Phosphorylation | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Q13043 (STK4) | Uniprot |
| T143 | Phosphorylation | Q05655 (PRKCD) , Q13043 (STK4) , P05771 (PRKCB) , P17252 (PRKCA) | Uniprot |
| S150 | Phosphorylation | O75914 (PAK3) , Q13131 (PRKAA1) | Uniprot |
| S166 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K177 | Acetylation | Uniprot | |
| K178 | Acetylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | P17252 (PRKCA) | Uniprot |
Research Backgrounds
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).
Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1.
Belongs to the troponin I family.
Research Fields
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
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