Product: Phospho-TIF1 beta (Ser824) Antibody
Catalog: AF8352
Description: Rabbit polyclonal antibody to Phospho-TIF1 beta (Ser824)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 88kDa; 89kD(Calculated).
Uniprot: Q13263
RRID: AB_2840414

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(88%)
Clonality:
Polyclonal
Specificity:
Phospho-TIF1 beta (Ser824) Antibody detects endogenous levels of TIF1 beta only when phosphorylated at Ser824.
RRID:
AB_2840414
Cite Format: Affinity Biosciences Cat# AF8352, RRID:AB_2840414.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 SUMO protein ligase TRIM28; E3 SUMO-protein ligase TRIM28; FLJ29029; KAP 1; KAP-1; KRAB associated protein 1; KRAB interacting protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP 1; KRIP-1; KRIP1; Nuclear corepressor KAP 1; Nuclear corepressor KAP-1; RING finger protein 96; RNF96; TF1B; TIF1 beta; TIF1-beta; TIF1B; TIF1B_HUMAN; Transcription intermediary factor 1 beta; Transcription intermediary factor 1-beta; Trim28; Tripartite motif containing 28; tripartite motif containing protein 28; Tripartite motif-containing protein 28;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q13263 TIF1B_HUMAN:

Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Sequence:
MAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Pig
75
Bovine
75
Sheep
75
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13263 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation
S9 Phosphorylation
S14 Phosphorylation
S17 Phosphorylation
S19 Phosphorylation
S26 Phosphorylation
K31 Acetylation
K31 Sumoylation
K31 Ubiquitination
T34 Phosphorylation
S37 Phosphorylation
S45 Phosphorylation
S49 Phosphorylation
S50 Phosphorylation
K127 Ubiquitination
Y133 Phosphorylation
S138 Phosphorylation
K188 Acetylation
K188 Sumoylation
K188 Ubiquitination
K199 Sumoylation
K213 Ubiquitination
K238 Ubiquitination
Y242 Phosphorylation
K254 Sumoylation
K254 Ubiquitination
S258 Phosphorylation
K261 Sumoylation
K261 Ubiquitination
K266 Acetylation
K266 Ubiquitination
K272 Ubiquitination
K275 Ubiquitination
S280 Phosphorylation
R282 Methylation
S285 Phosphorylation
K289 Acetylation
K289 Ubiquitination
K296 Ubiquitination
K304 Acetylation
K304 Ubiquitination
K308 Ubiquitination
K319 Sumoylation
K319 Ubiquitination
K337 Sumoylation
K337 Ubiquitination
K340 Acetylation
K340 Ubiquitination
S355 Phosphorylation
K365 Ubiquitination
K366 Sumoylation
K366 Ubiquitination
Y369 Phosphorylation
K377 Acetylation
K377 Sumoylation
K377 Ubiquitination
K390 Sumoylation
K390 Ubiquitination
K400 Ubiquitination
S401 Phosphorylation
K407 Ubiquitination
S417 Phosphorylation
R427 Methylation
S433 Phosphorylation
K434 Sumoylation
K434 Ubiquitination
S437 Phosphorylation
S439 Phosphorylation
S440 Phosphorylation
Y449 Phosphorylation P07948 (LYN)
S453 Phosphorylation
Y458 Phosphorylation P07948 (LYN)
S459 Phosphorylation
S460 Phosphorylation
S466 Phosphorylation
K469 Acetylation
K469 Sumoylation
K469 Ubiquitination
S471 Phosphorylation
S473 Phosphorylation Q05655 (PRKCD) , P49137 (MAPKAPK2) , O96017 (CHEK2) , O14757 (CHEK1)
S479 Phosphorylation
S489 Phosphorylation
T498 Phosphorylation
S501 Phosphorylation
K507 Sumoylation
K507 Ubiquitination
S512 Phosphorylation
T514 Phosphorylation
Y517 Phosphorylation P07948 (LYN)
T531 Phosphorylation
T536 Phosphorylation
T541 Phosphorylation
K554 Sumoylation
K575 Acetylation
K575 Sumoylation
K575 Ubiquitination
S594 Phosphorylation
S596 Phosphorylation
S598 Phosphorylation
T599 Phosphorylation
S600 Phosphorylation
S601 Phosphorylation
T611 Phosphorylation
S612 Phosphorylation
T620 Phosphorylation
S624 Phosphorylation
T626 Phosphorylation
K676 Sumoylation
S681 Phosphorylation
S683 Phosphorylation
S689 Phosphorylation
K695 Ubiquitination
S697 Phosphorylation
K750 Sumoylation
K750 Ubiquitination
S752 Phosphorylation
Y755 Phosphorylation
S756 Phosphorylation
S757 Phosphorylation
K770 Acetylation
K770 Sumoylation
K770 Ubiquitination
K774 Acetylation
K774 Sumoylation
K774 Ubiquitination
K779 Acetylation
K779 Sumoylation
K779 Ubiquitination
S784 Phosphorylation
R790 Methylation
K804 Sumoylation
K804 Ubiquitination
S816 Phosphorylation
S823 Phosphorylation
S824 Phosphorylation Q13315 (ATM)
S828 Phosphorylation

Research Backgrounds

Function:

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions. Acts as a corepressor for ZFP568 (By similarity).

(Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.

PTMs:

ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.

Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.

Auto-ubiquitinated; enhanced by MAGEA2 and MAGEC2.

Citrullinated by PADI4.

Subcellular Location:

Nucleus.
Note: Associated with centromeric heterochromatin during cell differentiation through CBX1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Subunit Structure:

Interacts with SETX. Oligomer; the RBCC domain homotrimerizes and interacts with one molecule of KRAB to form the KRAB-KAP1 corepressor complex. Binding to a KRAB domain is an absolute requirement for silencing gene expression. Interacts with CEBPB and NR3C1. Interacts with a number of KRAB-ZFP proteins including ZNF10, ZFP53, ZFP68, ZNF382 and ZNF256. Interacts with NCOR1, NR3C1 and CHD3. Interacts with CEBPB (via the RING-type and PHD-type zinc fingers). Component of a ternary complex that includes TRIM28, a HP1 protein (CBX1, CBX3 OR CBX5), a KRAB domain-containing protein, and DNA. Interacts with CBX5 (via the PxVxL motif); the interaction occurs in interphase nuclei and competes for binding POGZ. Interacts with POGZ; the interaction competes for interaction with CBX5. Interacts with SETDB1; the interaction is enhanced by KAP1 sumoylation, stimulates SETDB1 histone methyltransferase activity and gene silencing. Interacts (via the PHD-type zinc finger) with UBE2I; the interaction is required for sumoylation and repressor activity. Component of the TRIM28/KAP1-ERBB4-MDM2 complex involved in connecting growth factor and DNA damage responses. Interacts directly with ERBB4; the interaction represses ERBB4-mediated transcription activity. Interacts with MDM2; the interaction contributes to p53/TP53 inactivation. Component of the TRIM28/KAP1-MDM2-p53/TP53; involved in regulating p53/TP53 stabilization and activity. Interacts (via the leucine zipper alpha helical coiled-coil) with E2F1 (central region); the interaction inhibits E2F1 acetylation and transcriptional activity. Interacts with PPP1CA; the interaction dephosphorylates TRIM28 at Ser-824 and forms a complex at the p21 promoter site. Interacts with PPP1CB; the interaction is weak but is increased on dephosphorylation at Ser-824. Interacts with FES/FPS. Interacts with SMARCAD1. Interacts with, and sumoylates IRF7. Interacts with MAGEC2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with AICDA (By similarity). Interacts (via the RBCC domain) with KOX1 (via the KRAB domain), ZNF268 (via the KRAB domain) and ZNF300 (via the KRAB domain); the interactions increase KOX1, ZNF268 and ZNF300 nuclear localization activities. The large PER complex involved in the histone methylation is composed of at least PER2, CBX3, TRIM28, SUV39H1 and/or SUV39H2; CBX3 mediates the formation of the complex. Interacts with isoform 2 of ZFP90. Forms a complex with FOXP3 in the presence of isoform 2 of ZFP90. Interacts with NR4A3; the interactions potentiates NR4A3 activity on NurRE promoter (By similarity). Interacts (unphosphorylated or phosphorylated form) with ZBTB1 (via BTB domain). Probably part of a corepressor complex containing ZNF304, TRIM28, SETDB1 and DNMT1. Interacts with ATRX. Forms a complex with ATRX, SETDB1 and ZNF274. Interacts with ZFP568; the interaction mediates ZFP568 transcriptional repression activity (By similarity). Interacts with RRP1B. Interacts with CRY1 (By similarity).

(Microbial infection) Interacts with herpes virus 8 protein LANA1; this interaction facilitates establishment of viral latency.

Family&Domains:

The HP1 box is both necessary and sufficient for HP1 binding.

The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain.

The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization.

Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

Belongs to the TRIM/RBCC family.

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