Product: Phospho-Raptor (Ser792) Antibody
Catalog: AF8350
Description: Rabbit polyclonal antibody to Phospho-Raptor (Ser792)
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 149kDa; 149kD(Calculated).
Uniprot: Q8N122
RRID: AB_2840412

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 100ul $350 In stock
 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(86%), Zebrafish(86%), Bovine(86%), Horse(86%), Sheep(86%), Dog(86%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
Phospho-Raptor (Ser792) Antibody detects endogenous levels of Raptor only when phosphorylated at Ser792.
RRID:
AB_2840412
Cite Format: Affinity Biosciences Cat# AF8350, RRID:AB_2840412.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA1303; KOG1; Mip1; P150 target of rapamycin (TOR) scaffold protein; p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Raptor; Regulatory associated protein of mTOR; Regulatory associated protein of MTOR complex 1; Regulatory-associated protein of mTOR; RPTOR; RPTOR_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8N122 RPTOR_HUMAN:

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.

Sequence:
MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEVWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEATELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYHTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLYSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLVSDGSPMVRKELVVALSHLVVQYESNFCTVALQFIEEEKNYALPSPATTEGGSLTPVRDSPCTPRLRSVSSYGNIRAVATARSLNKSLQNLSLTEESGGAVAFSPGNLSTSSSASSTLGSPENEEHILSFETIDKMRRASSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDVAMKVLNSIAYKATVNARPQRVLDTSSLTQSAPASPTNKGVHIHQAGGSPPASSTSSSSLTNDVAKQPVSRDLPSGRPGTTGPAGAQYTPHSHQFPRTRKMFDKGPEQTADDADDAAGHKSFISATVQTGFCDWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNSRVRRQAQQVIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMSSGDVRIVRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALSECRVMTYREHTAWVVKASLQKRPDGHIVSVSVNGDVRIFDPRMPESVNVLQIVKGLTALDIHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Horse
86
Bovine
86
Sheep
86
Dog
86
Zebrafish
86
Chicken
86
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N122 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
K43 Ubiquitination
S48 Phosphorylation
K97 Ubiquitination
K108 Ubiquitination
K120 Ubiquitination
K131 Ubiquitination
K132 Ubiquitination
T159 Phosphorylation
K207 Ubiquitination
K297 Ubiquitination
K335 Ubiquitination
K603 Ubiquitination
Y692 Phosphorylation
S696 Phosphorylation P45983 (MAPK8) , P27361 (MAPK3) , P28482 (MAPK1) , P06493 (CDK1)
T699 Phosphorylation
T700 Phosphorylation
S704 Phosphorylation
T706 Phosphorylation P06493 (CDK1) , P45983 (MAPK8)
S711 Phosphorylation
T714 Phosphorylation
R718 Methylation
S719 Phosphorylation P51812 (RPS6KA3) , Q15418 (RPS6KA1)
S721 Phosphorylation P51812 (RPS6KA3) , Q15418 (RPS6KA1) , Q9UIK4 (DAPK2)
S722 Phosphorylation P51812 (RPS6KA3) , Q15418 (RPS6KA1) , P49841 (GSK3B) , Q13131 (PRKAA1)
Y723 Phosphorylation
S738 Phosphorylation
S771 Phosphorylation Q15759 (MAPK11)
S792 Phosphorylation O75385 (ULK1) , P49841 (GSK3B) , Q13131 (PRKAA1)
S795 Phosphorylation
S836 Phosphorylation
K840 Ubiquitination
T853 Phosphorylation
S854 Phosphorylation
S855 Phosphorylation O75385 (ULK1) , P42345 (MTOR)
T857 Phosphorylation
S859 Phosphorylation O75385 (ULK1) , P42345 (MTOR) , P49841 (GSK3B)
S863 Phosphorylation P54646 (PRKAA2) , P42345 (MTOR) , Q15759 (MAPK11) , P27361 (MAPK3) , P49841 (GSK3B) , P28482 (MAPK1) , P45983 (MAPK8) , Q9UBE8 (NLK)
T865 Phosphorylation
K867 Ubiquitination
S877 Phosphorylation P49841 (GSK3B)
S881 Phosphorylation
S882 Phosphorylation
T883 Phosphorylation
S884 Phosphorylation
S886 Phosphorylation
S887 Phosphorylation
T889 Phosphorylation
K894 Ubiquitination
T908 Phosphorylation Q9UPZ9 (ICK)
Y916 Phosphorylation
T917 Phosphorylation
K973 Ubiquitination
S982 Phosphorylation
K1008 Ubiquitination
K1030 Ubiquitination
K1053 Ubiquitination
K1056 Ubiquitination
K1097 Ubiquitination
K1157 Ubiquitination
S1189 Phosphorylation
Y1193 Phosphorylation
K1221 Ubiquitination

Research Backgrounds

Function:

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

PTMs:

Insulin-stimulated phosphorylation at Ser-863 by MTOR and MAPK8 up-regulates mTORC1 activity. Osmotic stress also induces phosphorylation at Ser-696, Thr-706 and Ser-863 by MAPK8. Ser-863 phosphorylation is required for phosphorylation at Ser-855 and Ser-859. In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1, which stimulates mTORC1 activity.

Subcellular Location:

Cytoplasm. Lysosome. Cytoplasmic granule.
Note: Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.

Subunit Structure:

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibition of its activity. Interacts with SPAG5; SPAG5 competes with MTOR for RPTOR-binding, resulting in decreased mTORC1 formation. Interacts with WAC; WAC positively regulates MTOR activity by promoting the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex which leads to the dimerization of the mTORC1 complex and its subsequent activation. Interacts with G3BP1. The complex formed with G3BP1 AND SPAG5 is increased by oxidative stress. Interacts with HTR6. Interacts with PIH1D1. Interacts with LARP1. Interacts with BRAT1. Interacts with SIK3.

(Microbial infection) Interacts with vaccinia virus protein F17; this interaction dysregulates mTOR.

Family&Domains:

Belongs to the WD repeat RAPTOR family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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