Product: Phospho-PFKFB2 (Ser466) Antibody
Catalog: AF8346
Description: Rabbit polyclonal antibody to Phospho-PFKFB2 (Ser466)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 58kDa; 58kD(Calculated).
Uniprot: O60825
RRID: AB_2840408

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PFKFB2 (Ser466) Antibody detects endogenous levels of PFKFB2 only when phosphorylated at Ser466.
RRID:
AB_2840408
Cite Format: Affinity Biosciences Cat# AF8346, RRID:AB_2840408.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
MSGASSSEQNNNSYETKTPNLRMSEKKCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYKSYDFFRHDNEEAMKIRKQCALVALEDVKAYLTEENGQIAVFDATNTTRERRDMILNFAEQNSFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYRPLDPDNYDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLMNIHVQPRTIYLCRHGESEFNLLGKIGGDSGLSVRGKQFAQALRKFLEEQEITDLKVWTSQLKRTIQTAESLGVPYEQWKILNEIDAGVCEEMTYAEIEKRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISHQAVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKLNVEAVNTHRDKPTNNFPKNQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRAQDMQEGAD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60825 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S13 Phosphorylation
S24 Phosphorylation
S32 Phosphorylation
S84 Phosphorylation
K86 Ubiquitination
K103 Ubiquitination
S175 Phosphorylation
K192 Ubiquitination
K211 Ubiquitination
K279 Ubiquitination
K287 Ubiquitination
K298 Ubiquitination
K305 Ubiquitination
Y356 Phosphorylation
Y358 Phosphorylation
Y366 Phosphorylation
T458 Phosphorylation
S466 Phosphorylation P23443 (RPS6KB1) , Q13131 (PRKAA1) , P31749 (AKT1) , P17612 (PRKACA) , P51812 (RPS6KA3) , P54646 (PRKAA2)
T468 Phosphorylation
S471 Phosphorylation
S472 Phosphorylation
T475 Phosphorylation
Y482 Phosphorylation
S483 Phosphorylation P31749 (AKT1)
S486 Phosphorylation
S493 Phosphorylation

Research Backgrounds

Function:

Synthesis and degradation of fructose 2,6-bisphosphate.

PTMs:

Phosphorylation by AMPK stimulates activity.

Tissue Specificity:

Heart.

Subunit Structure:

Homodimer.

Family&Domains:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.