Phospho-XRCC4 (Ser260) Antibody - #AF8336

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Product: Phospho-XRCC4 (Ser260) Antibody
Catalog: AF8336
Description: Rabbit polyclonal antibody to Phospho-XRCC4 (Ser260)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 40kDa; 38kD(Calculated).
Uniprot: Q13426
RRID: AB_2840398

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(80%), Sheep(80%), Rabbit(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
Phospho-XRCC4 (Ser260) Antibody detects endogenous levels of XRCC4 only when phosphorylated at Ser260.
RRID:
AB_2840398
Cite Format: Affinity Biosciences Cat# AF8336, RRID:AB_2840398.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA double strand break repair and V(D)J recombination protein XRCC4; DNA repair protein XRCC4; SSMED; X ray repair complementing defective repair in Chinese hamster cells 4; X ray repair cross complementing 4; X ray repair cross complementing protein 4; X-ray repair cross-complementing protein 4; XRCC 4; XRCC4; XRCC4_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q13426(XRCC4_HUMAN) >>Visit HPA database.

Gene(ID):
XRCC4(7518)
Expression:
Q13426 XRCC4_HUMAN:

Widely expressed.

Sequence:
MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Dog
90
Bovine
80
Sheep
80
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13426 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
T17 Phosphorylation
K65 Ubiquitination
K102 Ubiquitination
K146 Ubiquitination
K164 Ubiquitination
K169 Ubiquitination
K178 Ubiquitination
S193 Phosphorylation
K197 Ubiquitination
K210 Sumoylation
T222 Phosphorylation
Y229 Phosphorylation
S232 Phosphorylation
T233 Phosphorylation P68400 (CSNK2A1)
S237 Phosphorylation
T241 Phosphorylation
S256 Phosphorylation
S259 Phosphorylation
S260 Phosphorylation P78527 (PRKDC)
T264 Phosphorylation
K296 Ubiquitination
S299 Phosphorylation
S304 Phosphorylation P78527 (PRKDC)
S315 Phosphorylation
S320 Phosphorylation P78527 (PRKDC)
T323 Phosphorylation
S327 Phosphorylation P78527 (PRKDC)
S328 Phosphorylation P78527 (PRKDC)

Research Backgrounds

Function:

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

PTMs:

Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.

Monoubiquitinated.

Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Homodimer and homotetramer in solution. The homodimer associates with LIG4. The LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex composed of PRKDC, XRCC6/Ku70 and XRCC5/Ku86 to form the core non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. Interacts directly with PRKDC but not with the XRCC6/Ku70 and XRCC5/Ku86 dimer. Interacts with APTX and APLF.

Family&Domains:

Belongs to the XRCC4 family.

Research Fields

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

References

1). GCN5 mediates DNA-PKcs crotonylation for DNA double-strand break repair and determining cancer radiosensitivity. British journal of cancer, 2024 (PubMed: 38575732) [IF=8.8]

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