XRN2 Antibody - #AF0490

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Product: XRN2 Antibody
Catalog: AF0490
Description: Rabbit polyclonal antibody to XRN2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 108kDa; 109kD(Calculated).
Uniprot: Q9H0D6
RRID: AB_2834320

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
XRN2 Antibody detects endogenous levels of total XRN2.
RRID:
AB_2834320
Cite Format: Affinity Biosciences Cat# AF0490, RRID:AB_2834320.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5' 3' exoribonuclease 2; DHM1 like protein; DHP protein; XRN 2;

Immunogens

Immunogen:
Uniprot:

Q9H0D6(XRN2_HUMAN) >>Visit HPA database.

Gene(ID):
XRN2(22803)
Expression:
Q9H0D6 XRN2_HUMAN:

Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.

Description:
This gene shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known.
Sequence:
MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIHPCTHPEDKPAPKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFRASKEGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIADRLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLGLATHEPNFTIIREEFKPNKPKPCGLCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGEFIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHLPSLEIRENAIDRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKEKRKRMKRDQPAFTPSGILTPHALGSRNSPGSQVASNPRQAAYEMRMQNNSSPSISPNTSFTSDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQSYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMGVFPAASGNFLPPSWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRAALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELYQTGSTEPVEVPPELCHGIQGKFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAAVLKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVTYQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQNAAFQPNQYQMLAGPGGYPPRRDDRGGRQGYPREGRKYPLPPPSGRYNWN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H0D6 As Substrate

Site PTM Type Enzyme
K40 Ubiquitination
Y99 Phosphorylation
R120 Methylation
K123 Ubiquitination
K132 Sumoylation
K132 Ubiquitination
K142 Ubiquitination
K152 Ubiquitination
K172 Ubiquitination
Y176 Phosphorylation
Y177 Phosphorylation
K189 Ubiquitination
K207 Ubiquitination
K286 Ubiquitination
K368 Ubiquitination
K373 Ubiquitination
T374 Phosphorylation
T379 Phosphorylation
T433 Phosphorylation
S435 Phosphorylation
T439 Phosphorylation P50750 (CDK9) , P50613 (CDK7) , P28482 (MAPK1)
S445 Phosphorylation
S448 Phosphorylation
S451 Phosphorylation
S455 Phosphorylation
R458 Methylation
Y462 Phosphorylation
S470 Phosphorylation
S471 Phosphorylation
S473 Phosphorylation
S475 Phosphorylation
T478 Phosphorylation
S479 Phosphorylation
T481 Phosphorylation
S485 Phosphorylation
S487 Phosphorylation
K493 Ubiquitination
S499 Phosphorylation
S501 Phosphorylation
K517 Ubiquitination
K524 Methylation
K524 Ubiquitination
K533 Ubiquitination
K537 Ubiquitination
K616 Ubiquitination
K640 Acetylation
K640 Ubiquitination
K641 Ubiquitination
Y666 Phosphorylation
S678 Phosphorylation
K756 Ubiquitination
Y764 Phosphorylation
K767 Ubiquitination
K776 Ubiquitination
K782 Ubiquitination
S789 Phosphorylation
S790 Phosphorylation
K796 Acetylation
K796 Ubiquitination
R806 Methylation
R816 Methylation
R824 Methylation
R847 Methylation
R851 Methylation
K858 Methylation
S861 Phosphorylation
R864 Methylation
R870 Methylation
R880 Methylation
R883 Methylation
R895 Methylation
R921 Methylation
R922 Methylation
R925 Methylation
R928 Methylation
Y931 Phosphorylation
R933 Methylation
R946 Methylation

Research Backgrounds

Function:

Possesses 5'->3' exoribonuclease activity (By similarity). May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.

Subunit Structure:

Interacts with POLR2A and SMN1/SMN2. Interacts with CDKN2AIP and NKRF. Interacts with CDKN2AIPNL; the interaction is direct. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner.

Family&Domains:

Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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