Phospho-USF1 (Thr153) Antibody - #AF8331
| Product: | Phospho-USF1 (Thr153) Antibody |
| Catalog: | AF8331 |
| Description: | Rabbit polyclonal antibody to Phospho-USF1 (Thr153) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Rabbit, Dog |
| Mol.Wt.: | 34kDa; 34kD(Calculated). |
| Uniprot: | P22415 |
| RRID: | AB_2840393 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8331, RRID:AB_2840393.
Fold/Unfold
bHLHb11; Class B basic helix-loop-helix protein 11; FCHL 1; FCHL; FCHL1; HGNC:12593; HYPLIP 1; HYPLIP1; Major late transcription factor 1; Major late transcription factor; MLTF; MLTF I; MLTFI; Transcription factor USF-1; UEF; Upstream stimulatory factor 1; Upstream transcription factor 1; Upstream transcription factor 1, isoform CRA_a; USF 1; USF;
Immunogens
- P22415 USF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P22415 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T100 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | P49840 (GSK3A) , Q16539 (MAPK14) | Uniprot |
| S186 | Phosphorylation | P49840 (GSK3A) | Uniprot |
| S189 | Phosphorylation | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K235 | Acetylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| K286 | Ubiquitination | Uniprot | |
| K288 | Sumoylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K306 | Sumoylation | Uniprot | |
| S309 | Phosphorylation | Uniprot |
Research Backgrounds
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Nucleus.
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.
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For Research Use Only.
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