Phospho-ETV6 / Tel (Ser257) Antibody - #AF8325

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Product: Phospho-ETV6 / Tel (Ser257) Antibody
Catalog: AF8325
Description: Rabbit polyclonal antibody to Phospho-ETV6 / Tel (Ser257)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 53 KD; 53kD(Calculated).
Uniprot: P41212
RRID: AB_2840387

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Rabbit(89%), Dog(100%), Chicken(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Phospho-ETV6 / Tel (Ser257) Antibody detects endogenous levels of ETV6 / Tel only when phosphorylated at Ser257.
RRID:
AB_2840387
Cite Format: Affinity Biosciences Cat# AF8325, RRID:AB_2840387.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ETS related protein Tel1; ETS translocation variant 6; Ets variant 6; Ets variant gene 6 (TEL oncogene); ETS-related protein Tel1; ETV 6; ETV6; ETV6_HUMAN; Tel; TEL oncogene; TEL/ABL; TEL1; TEL1 oncogene; Transcription factor ETV 6; Transcription factor ETV6;

Immunogens

Immunogen:
Uniprot:

P41212(ETV6_HUMAN) >>Visit HPA database.

Gene(ID):
ETV6(2120)
Expression:
P41212 ETV6_HUMAN:

Ubiquitous.

Sequence:
MSETPAQCSIKQERISYTPPESPVPSYASSTPLHVPVPRALRMEEDSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFEMNGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKPRILFSPFFHPGNSIHTQPEVILHQNHEEDNCVQRTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSPDPEQRPLRSPLDNMIRRLSPAERAQGPRPHQENNHQESYPLSVSPMENNHCPASSESHPKPSSPRQESTRVIQLMPSPIMHPLILNPRHSVDFKQSRLSEDGLHREGKPINLSHREDLAYMNHIMVSVSPPEEHAMPIGRIADCRLLWDYVYQLLSDSRYENFIRWEDKESKIFRIVDPNGLARLWGNHKNRTNMTYEKMSRALRHYYKLNIIRKEPGQRLLFRFMKTPDEIMSGRTDRLEHLESQELDEQIYQEDEC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Chicken
100
Rabbit
89
Pig
88
Bovine
88
Xenopus
83
Zebrafish
67
Horse
50
Sheep
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41212 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T4 Phosphorylation
K11 Acetylation
K11 Sumoylation
S16 Phosphorylation
Y17 Phosphorylation
T18 Phosphorylation
S22 Phosphorylation Q16539 (MAPK14)
S26 Phosphorylation
Y27 Phosphorylation
S29 Phosphorylation
S30 Phosphorylation
T31 Phosphorylation
S47 Phosphorylation
Y60 Phosphorylation
K99 Sumoylation
S131 Phosphorylation
S139 Phosphorylation
T142 Phosphorylation
T161 Phosphorylation
S165 Phosphorylation
S182 Phosphorylation
S184 Phosphorylation
T187 Phosphorylation
T188 Phosphorylation
S193 Phosphorylation
S203 Phosphorylation
S213 Phosphorylation P27361 (MAPK3)
S238 Phosphorylation
S257 Phosphorylation Q16539 (MAPK14) , P27361 (MAPK3)
S271 Phosphorylation
S284 Phosphorylation
S293 Phosphorylation
K302 Acetylation
K302 Ubiquitination
Y314 Phosphorylation
S323 Phosphorylation
K384 Ubiquitination
K393 Ubiquitination
Y401 Phosphorylation
Y402 Phosphorylation
K403 Ubiquitination
K421 Sumoylation
K421 Ubiquitination
T422 Phosphorylation
S439 Phosphorylation
Y447 Phosphorylation

Research Backgrounds

Function:

Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.

PTMs:

Phosphorylation of Ser-257 by MAPK14 (p38) inhibits ETV6 transcriptional repression.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Can form homodimers or heterodimers with TEL2 or FLI1. Interacts with L3MBTL1 and HDAC9.

Family&Domains:

Belongs to the ETS family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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