Phospho-KAT13A/SRC1 (Thr1179) Antibody

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Product:	Phospho-KAT13A/SRC1 (Thr1179) Antibody
Catalog:	AF8319
Description:	Rabbit polyclonal antibody to Phospho-KAT13A/SRC1 (Thr1179)
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	200 kDa; 157kD(Calculated).
Uniprot:	Q15788
RRID:	AB_2840381

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%), Chicken(83%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

Phospho-KAT13A/SRC1 (Thr1179) Antibody detects endogenous levels of KAT13A/SRC1 only when phosphorylated at Thr1179.

RRID:

AB_2840381
Cite Format: Affinity Biosciences Cat# AF8319, RRID:AB_2840381.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bHLHe74; Class E basic helix-loop-helix protein 74; F SRC 1; Hin 2 protein; Hin2 protein; MGC129719; MGC129720; mNRC 1; NCoA 1; NCoA-1; Ncoa1; NCOA1_HUMAN; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; NY REN 52 antigen; Protein Hin 2; Protein Hin-2; Protein Hin2; Renal carcinoma antigen NY REN 52; Renal carcinoma antigen NY-REN-52; RIP 160; RIP160; SRC 1; SRC-1; Steroid receptor coactivator 1;

Immunogens

Immunogen:

Uniprot:

Q15788(NCOA1_HUMAN) >>Visit HPA database.

Gene(ID):

NCOA1(8648)

Expression:

Q15788 NCOA1_HUMAN:

Widely expressed.

Sequence:

Q15788 NCOA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEELAELLSANISDIDSLSVKPDKCKILKKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQGVIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENVTSYLGYNQEELMNTSVYSILHVGDHAEFVKNLLPKSLVNGVPWPQEATRRNSHTFNCRMLIHPPDEPGTENQEACQRYEVMQCFTVSQPKSIQEDGEDFQSCLICIARRLPRPPAITGVESFMTKQDTTGKIISIDTSSLRAAGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAHTKCKLCYPQSPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRVNPSVNPSISPAHGVARSSTLPPSNSNMVSTRINRQQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPRRQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPVLRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNTNPTPMTKPTPEEIKLEAQSQFTADLDQFDQLLPTLEKAAQLPGLCETDRMDGAVTSVTIKSEILPASLQSATARPTSRLNRLPELELEAIDNQFGQPGTGDQIPWTNNTVTAINQSKSEDQCISSQLDELLCPPTTVEGRNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLSERFPPQQATPPLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPRGAFSPGMGMQPRQTLNRPPAAPNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGINMRSGMQQQITPQPPLNAQMLAQRQRELYSQQHRQRQLIQQQRAMLMRQQSFGNNLPPSSGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQLAANPEASLANRNSMVSRGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPIPPPQSSLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGGNTNVQNMNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADGTQQVQQVQVFADVQCTVNLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15788 As Substrate

Q15788

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
S22	Phosphorylation		Uniprot
T26	Phosphorylation		Uniprot
S30	Phosphorylation		Uniprot
S103	Phosphorylation		Uniprot
S192	Phosphorylation		Uniprot
T209	Phosphorylation		Uniprot
S274	Phosphorylation		Uniprot
T277	Phosphorylation		Uniprot
S308	Phosphorylation		Uniprot
S325	Phosphorylation		Uniprot
S369	Phosphorylation		Uniprot
S372	Phosphorylation		Uniprot
S389	Phosphorylation		Uniprot
S393	Phosphorylation		Uniprot
S395	Phosphorylation	P28482 (MAPK1)	Uniprot
S423	Phosphorylation		Uniprot
S488	Phosphorylation		Uniprot
S517	Phosphorylation		Uniprot
S558	Phosphorylation		Uniprot
S565	Phosphorylation		Uniprot
S566	Phosphorylation		Uniprot
S569	Phosphorylation		Uniprot
S582	Phosphorylation		Uniprot
S620	Phosphorylation		Uniprot
K632	Acetylation		Uniprot
S659	Phosphorylation		Uniprot
S698	Phosphorylation		Uniprot
K732	Sumoylation		Uniprot
K774	Sumoylation		Uniprot
T789	Phosphorylation		Uniprot
S853	Phosphorylation		Uniprot
S863	Phosphorylation		Uniprot
K959	Ubiquitination		Uniprot
T979	Phosphorylation		Uniprot
S997	Phosphorylation		Uniprot
S1006	Phosphorylation		Uniprot
T1026	Phosphorylation		Uniprot
R1029	Methylation		Uniprot
S1033	Phosphorylation		Uniprot
R1041	Methylation		Uniprot
R1114	Methylation		Uniprot
R1131	Methylation		Uniprot
T1179	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
S1185	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
S1250	Phosphorylation		Uniprot
T1271	Phosphorylation		Uniprot
S1279	Phosphorylation		Uniprot
S1372	Phosphorylation		Uniprot
T1426	Phosphorylation		Uniprot

Research Backgrounds

Q15788_NCOA1_HUMAN

Function:

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

PTMs:

Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.

Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.

Subcellular Location:

Nucleus.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with the methyltransferase CARM1 (By similarity). Interacts with NCOA6 and NCOA2. Interacts with the FDL motif of STAT5A and STAT5B. Interacts with the LXXLL motif of STAT6. Interacts with STAT3 following IL-6 stimulation. Interacts with the basal transcription factor GTF2B. Interacts with the histone acetyltransferases EP300 and CREBBP. Interacts with PCAF, COPS5, NR3C1 and TTLL5/STAMP. Interacts with PSMB9. Interacts with UBE2L3; they functionally interact to regulate progesterone receptor transcriptional activity. Interacts with PRMT2 and DDX5. Interacts with ASXL1. Interacts with PRMT6. Interacts (via LXXLL 1, 2 and 3 motifs) with RORC (via AF-2 motif). Interacts in a ligand-dependent fashion with RXRA. Interacts with TRIP4. Interacts with NR4A3 (By similarity). Interacts with VDR.

Family&Domains:

The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.

Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.

Belongs to the SRC/p160 nuclear receptor coactivator family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

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For Research Use Only.
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Phospho-KAT13A/SRC1 (Thr1179) Antibody - #AF8319