BHLHB3 Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/3

AF0442 at 1/100 staining Mouse brain tissue by IHC-P.

Figure 1 BHLHE41 was low expressed in HCT116 and Lovo cells.

Product:	BHLHB3 Antibody
Catalog:	AF0442
Description:	Rabbit polyclonal antibody to BHLHB3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Dog
Mol.Wt.:	50kDa; 50kD(Calculated).
Uniprot:	Q9C0J9
RRID:	AB_2834318

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

BHLHB3 Antibody detects endogenous levels of total BHLHB3.

RRID:

AB_2834318
Cite Format: Affinity Biosciences Cat# AF0442, RRID:AB_2834318.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Basic helix-loop-helix domain containing class B3; BHE41_HUMAN; bHLH protein DEC2; bHLHB3; bHLHe41; Class B basic helix loop helix protein 3; Class B basic helix-loop-helix protein 3; Class E basic helix-loop-helix protein 41; DEC2; Differentially expressed in chondrocytes protein 2; Enhancer of split and hairy related protein 1; Enhancer-of-split and hairy-related protein 1; hDEC2; Moderately similar to basic-helix loop helix protein M.musculus; SHARP-1;

Immunogens

Immunogen:

Uniprot:

Q9C0J9(BHE41_HUMAN) >>Visit HPA database.

Gene(ID):

BHLHE41(79365)

Expression:

Q9C0J9 BHE41_HUMAN:

Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.

Description:

BHLHB3 May be a transcriptional repressor that represses both basal and activated transcription. Homodimerize.

Sequence:

Q9C0J9 BHE41_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDEGIPHLQERQLLEHRDFIGLDYSSLYMCKPKRSMKRDDTKDTYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLKALTALTEQQHQKIIALQNGERSLKSPIQSDLDAFHSGFQTCAKEVLQYLSRFESWTPREPRCVQLINHLHAVATQFLPTPQLLTQQVPLSKGTGAPSAAGSAAAPCLERAGQKLEPLAYCVPVIQRTQPSAELAAENDTDTDSGYGGEAEARPDREKGKGAGASRVTIKQEPPGEDSPAPKRMKLDSRGGGSGGGPGGGAAAAAAALLGPDPAAAAALLRPDAALLSSLVAFGGGGGAPFPQPAAAAAPFCLPFCFLSPSAAAAYVQPFLDKSGLEKYLYPAAAAAPFPLLYPGIPAPAAAAAAAAAAAAAAAAFPCLSSVLSPPPEKAGAAAATLLPHEVAPLGAPHPQHPHGRTHLPFAGPREPGNPESSAQEDPSQPGKEAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Dog

Pig

Chicken

Zebrafish

Horse

Sheep

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9C0J9 As Substrate

Q9C0J9

Site	PTM Type	Source
K42	Acetylation	Uniprot
K46	Acetylation	Uniprot
K76	Ubiquitination	Uniprot
S122	Phosphorylation	Uniprot
S151	Phosphorylation	Uniprot
K210	Sumoylation	Uniprot
T236	Phosphorylation	Uniprot
K266	Sumoylation	Uniprot
S274	Phosphorylation	Uniprot

Research Backgrounds

Q9C0J9_BHE41_HUMAN

Function:

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).

Subcellular Location:

Nucleus.

Tissue Specificity:

Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.

Subunit Structure:

Homodimer (By similarity). Heterodimer with BHLHE40/DEC1. Interacts with CIART and ARNTL/BMAL1 (By similarity). Interacts with RXRA. Interacts with NR0B2 and HNF1A (By similarity).

Research Fields

· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)

References

1). BHLHE41 Overexpression Alleviates the Malignant Behavior of Colon Cancer Cells Induced by Hypoxia via Modulating HIF-1α/EMT Pathway. Gastroenterology Research and Practice, 2022 (PubMed: 35615737) [IF=2.0]

Application: WB Species: Human Sample: colon cancer (CC) cells

Figure 1 BHLHE41 was low expressed in HCT116 and Lovo cells. (a) and (b) The level of BHLHE41 in colon cancer (CC) cells and normal cells was tested by Western blot and qRT-PCR. All experiments have been performed in triplicate, and data were expressed as mean ± standard deviation (SD).

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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BHLHB3 Antibody - #AF0442