Phospho-RAD50 (Ser635) Antibody | Affinity Biosciences

IHC
pElisa

1/5

AF8308 at 1/100 staining Human esophageal cancer by IHC-P.

AF8308 at 1/100 staining Human colorectal cancer by IHC-P.

AF8308 at 1/100 staining Rat heart tissue by IHC-P.

AF8308 at 1/100 staining Rat brain tissue by IHC-P.

peptide-ELISA analysis of AF8308.

Product:	Phospho-RAD50 (Ser635) Antibody
Catalog:	AF8308
Description:	Rabbit polyclonal antibody to Phospho-RAD50 (Ser635)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	154kDa; 154kD(Calculated).
Uniprot:	Q92878
RRID:	AB_2840370

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(88%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Phospho-RAD50 (Ser635) Antibody detects endogenous levels of RAD50 only when phosphorylated at Ser635.

RRID:

AB_2840370
Cite Format: Affinity Biosciences Cat# AF8308, RRID:AB_2840370.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNA repair protein RAD50; hRAD50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50, S. cerevisiae, homolog of; RAD50_HUMAN; RAD502; Rad50l; Truncated RAD50 protein;

Immunogens

Immunogen:

Uniprot:

Q92878(RAD50_HUMAN) >>Visit HPA database.

Gene(ID):

RAD50(10111)

Expression:

Q92878 RAD50_HUMAN:

Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

Sequence:

Q92878 RAD50_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGELIAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKAVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETLRQVRQTQGQKVKEYQMELKYLKQYKEKACEIRDQITSKEAQLTSSKEIVKSYENELDPLKNRLKEIEHNLSKIMKLDNEIKALDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLKQKQIDEIRDKKTGLGRIIELKSEILSKKQNELKNVKYELQQLEGSSDRILELDQELIKAERELSKAEKNSNVETLKMEVISLQNEKADLDRTLRKLDQEMEQLNHHTTTRTQMEMLTKDKADKDEQIRKIKSRHSDELTSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASSEQNKNHINNELKRKEEQLSSYEDKLFDVCGSQDFESDLDRLKEEIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPVCQRVFQTEAELQEVISDLQSKLRLAPDKLKSTESELKKKEKRRDEMLGLVPMRQSIIDLKEKEIPELRNKLQNVNRDIQRLKNDIEEQETLLGTIMPEEESAKVCLTDVTIMERFQMELKDVERKIAQQAAKLQGIDLDRTVQQVNQEKQEKQHKLDTVSSKIELNRKLIQDQQEQIQHLKSTTNELKSEKLQISTNLQRRQQLEEQTVELSTEVQSLYREIKDAKEQVSPLETTLEKFQQEKEELINKKNTSNKIAQDKLNDIKEKVKNIHGYMKDIENYIQDGKDDYKKQKETELNKVIAQLSECEKHKEKINEDMRLMRQDIDTQKIQERWLQDNLTLRKRNEELKEVEEERKQHLKEMGQMQVLQMKSEHQKLEENIDNIKRNHNLALGRQKGYEEEIIHFKKELREPQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVVMLKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGIIALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIVKCSVSSLGFNVH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92878 As Substrate

Q92878

Site	PTM Type	Enzyme	Source
K6	Ubiquitination		Uniprot
K20	Methylation		Uniprot
K62	Ubiquitination		Uniprot
K71	Ubiquitination		Uniprot
K112	Ubiquitination		Uniprot
K126	Ubiquitination		Uniprot
S128	Phosphorylation		Uniprot
S130	Phosphorylation		Uniprot
K132	Ubiquitination		Uniprot
K175	Ubiquitination		Uniprot
K187	Ubiquitination		Uniprot
R193	Methylation		Uniprot
T198	Phosphorylation		Uniprot
K211	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
K238	Ubiquitination		Uniprot
K242	Ubiquitination		Uniprot
K252	Ubiquitination		Uniprot
K256	Ubiquitination		Uniprot
S263	Phosphorylation		Uniprot
K264	Acetylation		Uniprot
K273	Ubiquitination		Uniprot
K280	Ubiquitination		Uniprot
K295	Ubiquitination		Uniprot
Y308	Phosphorylation		Uniprot
K343	Ubiquitination		Uniprot
R359	Methylation		Uniprot
K398	Acetylation		Uniprot
K446	Ubiquitination		Uniprot
K453	Acetylation		Uniprot
K461	Acetylation		Uniprot
K461	Ubiquitination		Uniprot
S470	Phosphorylation		Uniprot
S471	Phosphorylation		Uniprot
R473	Methylation		Uniprot
K483	Ubiquitination		Uniprot
K520	Ubiquitination		Uniprot
K585	Acetylation		Uniprot
K599	Ubiquitination		Uniprot
K616	Ubiquitination		Uniprot
S623	Phosphorylation		Uniprot
S624	Phosphorylation		Uniprot
S635	Phosphorylation	Q13315 (ATM) , Q13535 (ATR)	Uniprot
S640	Phosphorylation		Uniprot
T690	Phosphorylation		Uniprot
K743	Ubiquitination		Uniprot
K753	Ubiquitination		Uniprot
R797	Methylation		Uniprot
R823	Methylation		Uniprot
K838	Ubiquitination		Uniprot
K845	Ubiquitination		Uniprot
K851	Ubiquitination		Uniprot
K864	Ubiquitination		Uniprot
K921	Ubiquitination		Uniprot
K959	Acetylation		Uniprot
Y964	Phosphorylation		Uniprot
K982	Ubiquitination		Uniprot
K1032	Acetylation		Uniprot
K1059	Ubiquitination		Uniprot
K1068	Ubiquitination		Uniprot
K1089	Ubiquitination		Uniprot
T1113	Phosphorylation		Uniprot
T1114	Phosphorylation		Uniprot
K1119	Ubiquitination		Uniprot
Y1125	Phosphorylation		Uniprot
K1126	Ubiquitination		Uniprot
K1134	Ubiquitination		Uniprot
Y1162	Phosphorylation		Uniprot
K1178	Ubiquitination		Uniprot
K1190	Ubiquitination		Uniprot
K1285	Acetylation		Uniprot
K1285	Ubiquitination		Uniprot
K1291	Ubiquitination		Uniprot
K1301	Ubiquitination		Uniprot

Research Backgrounds

Q92878_RAD50_HUMAN

Function:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Subcellular Location:

Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.

Tissue Specificity:

Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

Subunit Structure:

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM8 and MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. Interacts with MRNIP.

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

Family&Domains:

The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11 homodimer (By similarity).

Belongs to the SMC family. RAD50 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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Phospho-RAD50 (Ser635) Antibody - #AF8308