Phospho-PDE6G (Thr22) Antibody | Affinity Biosciences

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Product:	Phospho-PDE6G (Thr22) Antibody
Catalog:	AF8299
Description:	Rabbit polyclonal antibody to Phospho-PDE6G (Thr22)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	9KD; 10kD(Calculated).
Uniprot:	P18545
RRID:	AB_2840361

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Phospho-PDE6G (Thr22) Antibody detects endogenous levels of PDE6G only when phosphorylated at Thr22.

RRID:

AB_2840361
Cite Format: Affinity Biosciences Cat# AF8299, RRID:AB_2840361.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5''-cyclic phosphodiesterase subunit gamma; CNRG_HUMAN; GMP-PDE gamma; MGC125749; PDE 6; PDE6G; PDEG; Phosphodiesterase 6G cGMP Specific; Phosphodiesterase 6G cGMP specific rog gamma; Retinal Rod Photoreceptor cGMP Phosphodiesterase gamma; Retinal rod rhodopsin sensitive cGMP 3' 5'vcyclic phosphodiesterase subunit gamma; Retinal rod rhodopsin-sensitive cGMP 3''; Rod cG PDE G; RP57;

Immunogens

Immunogen:

Uniprot:

P18545(CNRG_HUMAN) >>Visit HPA database.

Gene(ID):

PDE6G(5148)

Sequence:

P18545 CNRG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNLEPPKAEFRSATRVAGGPVTPRKGPPKFKQRQTRQFKSKPPKKGVQGFGDDIPGMEGLGTDITVICPWEAFNHLELHELAQYGII

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Chicken

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18545 As Substrate

P18545

Site	PTM Type	Enzyme	Source
T22	Phosphorylation	P27361 (MAPK3)	Uniprot
K25	Ubiquitination		Uniprot
R36	Methylation		Uniprot
T62	Phosphorylation	A0A0S2Z3I6 (ADRBK1) , P25098 (GRK2)	Uniprot

Research Backgrounds

P18545_CNRG_HUMAN

Function:

Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.

Subunit Structure:

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Family&Domains:

Belongs to the rod/cone cGMP-PDE gamma subunit family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Sensory system > Phototransduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-PDE6G (Thr22) Antibody - #AF8299