Phospho-p73 (Thr86) Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat heart, using Phospho-p73 (Thr86) Antibody.

Product:	Phospho-p73 (Thr86) Antibody
Catalog:	AF8296
Description:	Rabbit polyclonal antibody to Phospho-p73 (Thr86)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	80kDa; 70kD(Calculated).
Uniprot:	O15350
RRID:	AB_2840358

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-p73 (Thr86) Antibody detects endogenous levels of p73 only when phosphorylated at Thr86.

RRID:

AB_2840358
Cite Format: Affinity Biosciences Cat# AF8296, RRID:AB_2840358.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Delta N p73; DNp73; p53 like transcription factor; p53 related protein; P73; TP73;

Immunogens

Immunogen:

Uniprot:

O15350(P73_HUMAN) >>Visit HPA database.

Gene(ID):

TP73(7161)

Expression:

O15350 P73_HUMAN:

Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.

Sequence:

O15350 P73_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Pig

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15350 As Substrate

O15350

Site	PTM Type	Enzyme	Source
T14	Phosphorylation		Uniprot
T27	Phosphorylation	P53350 (PLK1)	Uniprot
Y28	Phosphorylation	P08631 (HCK)	Uniprot
S47	Phosphorylation	O14757 (CHEK1)	Uniprot
S48	Phosphorylation	Q9NYY3 (PLK2)	Uniprot
T86	Phosphorylation	P06493 (CDK1) , P24941 (CDK2)	Uniprot
Y99	Phosphorylation	P00519 (ABL1) , A0A173G4P4 (Abl fusion)	Uniprot
T167	Phosphorylation		Uniprot
T173	Phosphorylation		Uniprot
S235	Phosphorylation	O14965 (AURKA)	Uniprot
S289	Phosphorylation	Q05655 (PRKCD)	Uniprot
K321	Acetylation		Uniprot
K327	Acetylation		Uniprot
K331	Acetylation		Uniprot
S333	Phosphorylation		Uniprot
K345	Ubiquitination		Uniprot
S388	Phosphorylation	P05771-2 (PRKCB) , P17252 (PRKCA)	Uniprot
S426	Phosphorylation	Q13131 (PRKAA1)	Uniprot
S471	Phosphorylation		Uniprot
T568	Phosphorylation		Uniprot
K627	Sumoylation		Uniprot

Research Backgrounds

O15350_P73_HUMAN

Function:

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.

PTMs:

Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function.

Higher levels of phosphorylation seen in the brain from patients with Huntington disease.

Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Accumulates in the nucleus in response to DNA damage.

Tissue Specificity:

Subunit Structure:

Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation.

(Microbial infection) Interacts with Epstein-Barr virus protein EBNA6; this interaction inhibits TP73-mediated apoptotic pathway.

Family&Domains:

Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL1 tyrosine kinase SH3 domain.

The PPxY motif mediates interaction with WWOX.

Belongs to the p53 family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

References

1). CDK4/6 inhibition suppresses p73 phosphorylation and activates DR5 to potentiate chemotherapy and immune checkpoint blockade. CANCER RESEARCH, 2022 (PubMed: 35149588) [IF=11.2]

Application: WB Species: Mice Sample: tumors tissue

Figure 5. DR5 is required for the antitumor activity of combining Palbo and TRAIL or 5-FU in mice. (A)-(C) Nude mice with established WT or DR5-KO HCT116 xenografts were treated with TRAIL (i.p.; 100 μg/mice every other day), Palbo (oral gavage;150 mg/kg daily), or their combination as indicated. (A) Tumor volume at indicated time points with statistical significance for indicated comparisons (N=6 in each group). (B) Western blotting of DR5 and p-p73 (Thr86) in randomly selected WT tumors treated as in (A) for 4 consecutive days. (C) Tumor sections from mice treated as in (B) were analyzed for apoptosis by active caspase 3 staining (left panel) and quantification (right panel). Scale bars: 25 μm. (D)-(F) Nude mice with established WT or DR5-KO HCT116 xenografts were treated with 5-FU (i.p.; 25 mg/kg every other day), Palbo (oral gavage;150 mg/kg daily), or their combination as indicated. (D) Tumor volume at indicated time points with statistical significance for indicated comparisons (N=6 in each group). (E) Western blotting of DR5 in randomly selected WT tumors treated as in (D) for 4 consecutive days. (F) Tumor sections from mice treated as in (E) were analyzed for apoptosis by active caspase 3 staining (left panel) and quantification (right panel). Scale bars: 25 μm. In (C) and (F), arrows indicate examples of positive signals. Bar graphs show results of 300 cells in each mouse (N=3 in each group). *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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Phospho-p73 (Thr86) Antibody - #AF8296