Phospho-NACA (Ser43) Antibody - #AF8290

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Product: Phospho-NACA (Ser43) Antibody
Catalog: AF8290
Description: Rabbit polyclonal antibody to Phospho-NACA (Ser43)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Chicken
Mol.Wt.: 24KD; 23kD(Calculated).
Uniprot: Q13765
RRID: AB_2840352

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
Phospho-NACA (Ser43) Antibody detects endogenous levels of NACA only when phosphorylated at Ser43.
RRID:
AB_2840352
Cite Format: Affinity Biosciences Cat# AF8290, RRID:AB_2840352.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha NAC; Alpha-NAC; Hom s 2.02; HSD48; MGC117224; NAC-alpha; naca; NACA/1.9.2; NACA_HUMAN; NACA1; Nascent polypeptide associated complex alpha polypeptide; Nascent polypeptide associated complex alpha subunit; Nascent polypeptide associated complex subunit alpha; Nascent polypeptide-associated complex subunit alpha;

Immunogens

Immunogen:

A synthesized peptide derived from human NACA around the phosphorylation site of Ser43.

Uniprot:

Q13765(NACA_HUMAN) >>Visit HPA database.

Gene(ID):
NACA(4666)
Expression:
Q13765 NACA_HUMAN:

Ubiquitously expressed.

Sequence:
MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Chicken
86
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13765 As Substrate

Site PTM Type Enzyme
S43 Phosphorylation Q13418 (ILK)
S132 Phosphorylation
K142 Acetylation
T159 Phosphorylation
T161 Phosphorylation
S166 Phosphorylation
S186 Phosphorylation
S191 Phosphorylation
S203 Phosphorylation

Research Backgrounds

Function:

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.

PTMs:

Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.

Subcellular Location:

Cytoplasm. Nucleus.
Note: The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with TBP and JUN (By similarity). Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit and contacts the ribosomal protein L23, which is positioned near the exiting site. Both subunits can contact nascent polypeptide chains. NACA may also form homodimers, and only this form binds DNA.

Family&Domains:

The positively charged inner surface of the NAC-A/B domain is crucial for NACA localization in the nucleus and DNA-binding. This region is blocked from binding nucleic acids in the heterodimeric complex by a helix region in the beta-subunit, it also displays much higher affinity for RNA than DNA.

Belongs to the NAC-alpha family.

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