Phospho-MCL1 (Thr163) Antibody | Affinity Biosciences

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Product:	Phospho-MCL1 (Thr163) Antibody
Catalog:	AF8284
Description:	Rabbit polyclonal antibody to Phospho-MCL1 (Thr163)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	35kDa; 37kD(Calculated).
Uniprot:	Q07820
RRID:	AB_2840346

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-MCL1 (Thr163) Antibody detects endogenous levels of MCL1 only when phosphorylated at Thr163.

RRID:

AB_2840346
Cite Format: Affinity Biosciences Cat# AF8284, RRID:AB_2840346.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Bcl 2 related protein EAT/mcl1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; BCL2 related; Bcl2-L-3; BCL2L3; EAT; Induced myeloid leukemia cell differentiation protein Mcl 1; Induced myeloid leukemia cell differentiation protein Mcl-1; MCL 1; MCL1; MCL1-ES; mcl1/EAT; MCL1_HUMAN; MCL1L; MCL1S; MGC104264; MGC1839; Myeloid Cell Leukemia 1; Myeloid cell leukemia ES; Myeloid cell leukemia sequence 1; Myeloid cell leukemia sequence 1 BCL2 related; Myeloid cell leukemia sequence 1 isoform 1; OTTHUMP00000032794; OTTHUMP00000032795; TM;

Immunogens

Immunogen:

Uniprot:

Q07820(MCL1_HUMAN) >>Visit HPA database.

Gene(ID):

MCL1(4170)

Sequence:

Q07820 MCL1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYLIR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07820 As Substrate

Q07820

Site	PTM Type	Enzyme	Source
K5	Methylation		Uniprot
K5	Ubiquitination		Uniprot
K40	Ubiquitination		Uniprot
S64	Phosphorylation	P45983 (MAPK8) , P24941 (CDK2) , P06493 (CDK1)	Uniprot
T68	Phosphorylation		Uniprot
T70	Phosphorylation		Uniprot
T92	Phosphorylation	P06493 (CDK1) , P27361 (MAPK3) , P24941 (CDK2)	Uniprot
S121	Phosphorylation	P45983 (MAPK8) , P24941 (CDK2) , P53779 (MAPK10)	Uniprot
Y129	Phosphorylation		Uniprot
K136	Ubiquitination		Uniprot
S150	Phosphorylation		Uniprot
T154	Phosphorylation		Uniprot
T156	Phosphorylation		Uniprot
S159	Phosphorylation	P49840 (GSK3A) , P49841 (GSK3B)	Uniprot
S162	Phosphorylation		Uniprot
T163	Phosphorylation	P24941 (CDK2) , P53779 (MAPK10) , P27361 (MAPK3) , P45983 (MAPK8) , P28482 (MAPK1)	Uniprot
K194	Ubiquitination		Uniprot
K197	Ubiquitination		Uniprot
K234	Ubiquitination		Uniprot
K238	Ubiquitination		Uniprot
K244	Ubiquitination		Uniprot
S245	Phosphorylation		Uniprot
K276	Ubiquitination		Uniprot
K279	Ubiquitination		Uniprot
K302	Ubiquitination		Uniprot
K308	Ubiquitination		Uniprot
Y347	Phosphorylation		Uniprot

Research Backgrounds

Q07820_MCL1_HUMAN

Function:

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

PTMs:

Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.

Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.

Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.

Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.

Subcellular Location:

Membrane>Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus>Nucleoplasm.
Note: Cytoplasmic, associated with mitochondria.

Subunit Structure:

Interacts with HIF3A (via C-terminus domain) (By similarity). Interacts with BAD, BOK, BIK and BMF (By similarity). Interacts with PMAIP1. Interacts with BBC3 (By similarity). Isoform 1 interacts with BAX, BAK1 and TPT1. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptotic BCL2-related proteins. Interacts with RTL10/BOP. Interacts with BCL2L11; may sequester BCL2L11 to prevent its pro-apoptotic activity. Interacts with GIMAP5 and HSPA8/HSC70; the interaction between HSPA8 and MCL1 is impaired in the absence of GIMAP5 (By similarity).

Family&Domains:

Belongs to the Bcl-2 family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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Phospho-MCL1 (Thr163) Antibody - #AF8284