Phospho-LAB (Tyr136) Antibody | Affinity Biosciences

pElisa

1/1

Product:	Phospho-LAB (Tyr136) Antibody
Catalog:	AF8278
Description:	Rabbit polyclonal antibody to Phospho-LAB (Tyr136)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	26KD; 27kD(Calculated).
Uniprot:	Q9GZY6
RRID:	AB_2840340

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Horse(100%), Sheep(89%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-LAB (Tyr136) Antibody detects endogenous levels of LAB only when phosphorylated at Tyr136.

RRID:

AB_2840340
Cite Format: Affinity Biosciences Cat# AF8278, RRID:AB_2840340.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HSPC046; LAB; LAT2; Linker for activation of B cells; Linker for activation of B-cells; Linker for activation of T cells family member 2; Linker for activation of T cells transmembrane adaptor 2; Linker for activation of T-cells family member 2; Membrane associated adapter molecule; Membrane-associated adapter molecule; Non T cell activation linker; Non-T-cell activation linker; NTAL_HUMAN; WBSCR 5; Wbscr15; Williams Beuren syndrome chromosome region 15; Williams Beuren syndrome chromosome region 5; Williams-Beuren syndrome chromosomal region 15 protein; Williams-Beuren syndrome chromosomal region 5 protein; WSCR5;

Immunogens

Immunogen:

Uniprot:

Q9GZY6(NTAL_HUMAN) >>Visit HPA database.

Gene(ID):

LAT2(7462)

Expression:

Q9GZY6 NTAL_HUMAN:

Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).

Sequence:

Q9GZY6 NTAL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSGTELLWPGAALLVLLGVAASLCVRCSRPGAKRSEKIYQQRSLREDQQSFTGSRTYSLVGQAWPGPLADMAPTRKDKLLQFYPSLEDPASSRYQNFSKGSRHGSEEAYIDPIAMEYYNWGRFSKPPEDDDANSYENVLICKQKTTETGAQQEGIGGLCRGDLSLSLALKTGPTSGLCPSASPEEDEESEDYQNSASIHQWRESRKVMGQLQREASPGPVGSPDEEDGEPDYVNGEVAATEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Pig

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZY6 As Substrate

Q9GZY6

Site	PTM Type	Enzyme	Source
Y40	Phosphorylation		Uniprot
S44	Phosphorylation		Uniprot
S51	Phosphorylation		Uniprot
S55	Phosphorylation		Uniprot
T57	Phosphorylation		Uniprot
Y58	Phosphorylation		Uniprot
S59	Phosphorylation		Uniprot
K77	Ubiquitination		Uniprot
K79	Ubiquitination		Uniprot
Y84	Phosphorylation		Uniprot
S86	Phosphorylation		Uniprot
Y95	Phosphorylation	P07948 (LYN)	Uniprot
K100	Ubiquitination		Uniprot
S102	Phosphorylation		Uniprot
S106	Phosphorylation		Uniprot
Y110	Phosphorylation	P10721 (KIT) , P07948 (LYN)	Uniprot
Y118	Phosphorylation	P10721 (KIT) , P07948 (LYN)	Uniprot
Y119	Phosphorylation	P07948 (LYN)	Uniprot
K126	Ubiquitination		Uniprot
S135	Phosphorylation		Uniprot
Y136	Phosphorylation	P07948 (LYN) , P43405 (SYK)	Uniprot
S167	Phosphorylation		Uniprot
S183	Phosphorylation		Uniprot
S190	Phosphorylation		Uniprot
Y193	Phosphorylation	P43405 (SYK)	Uniprot
S196	Phosphorylation		Uniprot
S198	Phosphorylation		Uniprot
S205	Phosphorylation		Uniprot
S217	Phosphorylation		Uniprot
Y233	Phosphorylation	P43405 (SYK)	Uniprot

Research Backgrounds

Q9GZY6_NTAL_HUMAN

Function:

Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.

PTMs:

Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.

May be polyubiquitinated.

Subcellular Location:

Cell membrane>Single-pass type III membrane protein.
Note: Present in lipid rafts.

Tissue Specificity:

Subunit Structure:

When phosphorylated, interacts with GRB2. May also interact with SOS1, GAB1 and CBL.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-LAB (Tyr136) Antibody - #AF8278