Phospho-Kizuna (Thr379) Antibody - #AF8277

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Product: Phospho-Kizuna (Thr379) Antibody
Catalog: AF8277
Description: Rabbit polyclonal antibody to Phospho-Kizuna (Thr379)
Application: WB
Reactivity: Human
Prediction: Rat, Pig, Xenopus
Mol.Wt.: 75KD; 75kD(Calculated).
Uniprot: Q2M2Z5
RRID: AB_2840339

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rat(%), Pig(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
Phospho-Kizuna (Thr379) Antibody detects endogenous levels of Kizuna only when phosphorylated at Thr379.
RRID:
AB_2840339
Cite Format: Affinity Biosciences Cat# AF8277, RRID:AB_2840339.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Centrosomal protein kizuna; DKFZP586H021; HT013; Kiz; KIZ_HUMAN; KIZUNA; MGC102941; MGC141930; NCRNA00153; Plk1s1; Polo-like kinase 1 substrate 1; Uncharacterized protein C20orf19;

Immunogens

Immunogen:
Uniprot:

Q2M2Z5(KIZ_HUMAN) >>Visit HPA database.

Gene(ID):
KIZ(55857)
Sequence:
MSRTLASAVPLSSPDYYERLGQLQHGLRDSEKKRLDLEKKLYEYNQSDTCRVKLKYVKLKNYLKEICESEKKAHTRNQEYLKRFERVQAHVVHFTTNTEKLQKLKLEYETQIKKMLCSKDSLGLKEELTDEDREKVAVHEGINSGTAMSRGLYQPATIFMGRQMSAILSMRDFSTEHKSPQPTKNFSIPDPHSHRQTAQSSNVTDSCVVQTSNDTQCLNKSDNIDGKASLQIGEKMPVTASVLSEEEQTHCLEIGSNTRHGKSNLSEGKKSAELNSPLRERLSPENRTTDLKCDSSSGSEGEILTREHIEVEEKRASPPVSPIPVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISISEDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPGQTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEAKPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHLSGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNHNTDDSDDFYD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Xenopus
88
Horse
75
Bovine
75
Sheep
75
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q2M2Z5 As Substrate

Site PTM Type Enzyme
K40 Ubiquitination
Y42 Phosphorylation
K64 Ubiquitination
K125 Ubiquitination
S165 Phosphorylation
S179 Phosphorylation
S263 Phosphorylation
S266 Phosphorylation
S276 Phosphorylation
S283 Phosphorylation
S317 Phosphorylation
S321 Phosphorylation
T379 Phosphorylation P53350 (PLK1)
S435 Phosphorylation
S436 Phosphorylation
S439 Phosphorylation
T455 Phosphorylation
S458 Phosphorylation
S478 Phosphorylation
K568 Ubiquitination
S594 Phosphorylation
S600 Phosphorylation
T605 Phosphorylation
S616 Phosphorylation
S618 Phosphorylation
S619 Phosphorylation
S620 Phosphorylation
S623 Phosphorylation
S647 Phosphorylation
S650 Phosphorylation
S652 Phosphorylation
T665 Phosphorylation
S668 Phosphorylation

Research Backgrounds

Function:

Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.

PTMs:

Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with AKAP9, CEP72, ODF2, PCNT and TUBGCP2.

Family&Domains:

Belongs to the kizuna family.

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