RHOBTB1/2 Antibody - #AF0450

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Product: RHOBTB1/2 Antibody
Catalog: AF0450
Description: Rabbit polyclonal antibody to RHOBTB1/2
Application: WB IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 83kDa; 83kD,79kD(Calculated).
Uniprot: Q9BYZ6 | O94844
RRID: AB_2834314

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
RHOBTB1/2 Antibody detects endogenous levels of total RHOBTB1/2.
RRID:
AB_2834314
Cite Format: Affinity Biosciences Cat# AF0450, RRID:AB_2834314.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DBC 2; Deleted in breast cancer 2; Deleted in breast cancer 2 gene protein; p83; RHBT2_HUMAN; Rho related BTB domain containing 2; Rho related BTB domain containing protein 2; Rho-related BTB domain-containing protein 2; RHOBTB 2; Rhobtb2; 1700008H16Rik; 3110048G13Rik; AI173445; AI573858; AV350930; KIAA0740; MGC107538; MGC33059; MGC33841; OTTHUMP00000019662; OTTHUMP00000019663; RHBT1_HUMAN; Rho related BTB domain containing 1; Rho related BTB domain containing protein 1; Rho-related BTB domain-containing protein 1; Rhobtb1;

Immunogens

Immunogen:

A synthesized peptide derived from human RHBT2, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9BYZ6(RHBT2_HUMAN) >>Visit HPA database.

O94844(RHBT1_HUMAN) >>Visit HPA database.

Gene(ID):
RHOBTB1(9886) | RHOBTB2(23221)
Expression:
Q9BYZ6 RHBT2_HUMAN:

Ubiquitous, with highest levels in neural tissues. Expression is also detected in fetal lung, heart, and brain.

O94844 RHBT1_HUMAN:

Ubiquitous, with highest levels in skeletal muscle, placenta, testis, stomach, and kidney, followed by uterus and adrenal gland. Expressed in a variety of fetal tissues.

Description:
RhoBTB2 Belongs to the small GTPase superfamily. Rho family.
Sequence:
MDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFAHKIYLSTSSSKFYDLFLMDLSEGELGGPSEPGGTHPEDHQGHSDQHHHHHHHHHGRDFLLRAASFDVCESVDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTYKSRLMVVVKMDSSIQPGPFRAVLKYLYTGELDENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYTGMFTSSPDLDDMKLIILANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADWCLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLKRQPKRRWLFWNSPSSPSSSAASSSSPSSSSAVV

MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQADQWKSSNKSLVEALGLEAEGAVPETQTLTGWSKGFIGMHREMQVNPISKRMGPMTVVRMDASVQPGPFRTLLQFLYTGQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLSKGTFSDVTFKLDDGAISAHKPLLICSCEWMAAMFGGSFVESANSEVYLPNINKISMQAVLDYLYTKQLSPNLDLDPLELIALANRFCLPHLVALAEQHAVQELTKAATSGVGIDGEVLSYLELAQFHNAHQLAAWCLHHICTNYNSVCSKFRKEIKSKSADNQEYFERHRWPPVWYLKEEDHYQRVKREREKEDIALNKHRSRRKWCFWNSSPAVA

PTMs - Q9BYZ6/O94844 As Substrate

Site PTM Type Enzyme
T120 Phosphorylation
T376 Phosphorylation
S388 Phosphorylation
S390 Phosphorylation
Y432 Phosphorylation
Y434 Phosphorylation
T435 Phosphorylation
K687 Ubiquitination
Site PTM Type Enzyme
S69 Phosphorylation
K119 Ubiquitination
S352 Phosphorylation
T398 Phosphorylation
S480 Phosphorylation
T483 Phosphorylation
S485 Phosphorylation
Y542 Phosphorylation
Y544 Phosphorylation
K679 Ubiquitination

Research Backgrounds

Tissue Specificity:

Ubiquitous, with highest levels in neural tissues. Expression is also detected in fetal lung, heart, and brain.

Subunit Structure:

Interacts with HSP90AA1 and HSP90AB1. Interacts with CUL3.

Family&Domains:

Belongs to the small GTPase superfamily. Rho family.

Tissue Specificity:

Ubiquitous, with highest levels in skeletal muscle, placenta, testis, stomach, and kidney, followed by uterus and adrenal gland. Expressed in a variety of fetal tissues.

Family&Domains:

Belongs to the small GTPase superfamily. Rho family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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