Phospho-Calcipressin 1 (Ser163) Antibody

IHC
pElisa
Cites

1/3

AF8268 at 1/100 staining Rat skin tissue by IHC-P.

Figure 4 Mangiferin alleviated PDGF-BB-induced mitochondrial fission and increase in ROS levels.

Product:	Phospho-Calcipressin 1 (Ser163) Antibody
Catalog:	AF8268
Description:	Rabbit polyclonal antibody to Phospho-Calcipressin 1 (Ser163)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	22 KD; 28kD(Calculated).
Uniprot:	P53805
RRID:	AB_2840330

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

Phospho-Calcipressin 1 (Ser163) Antibody detects endogenous levels of Calcipressin 1 only when phosphorylated at Ser163.

RRID:

AB_2840330
Cite Format: Affinity Biosciences Cat# AF8268, RRID:AB_2840330.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Adapt 78; Adapt78; Calcipressin 1; Calcipressin-1; Calcium and oxidant inducible mRNA; CSP 1; CSP1; Down syndrome candidate region 1; Down syndrome critical region gene 1; Down syndrome critical region protein 1; DSC 1; DSC1; DSCR 1; DSCR1; MCIP 1; MCIP1; Modulatory calcineurin interacting protein 1; Myocyte enriched calcineurin interacting protein 1; Myocyte-enriched calcineurin-interacting protein 1; Near DSCR proline rich protein; RCAN1; RCAN1_HUMAN; RCN 1; RCN1; Regulator of calcineurin 1;

Immunogens

Immunogen:

Uniprot:

P53805(RCAN1_HUMAN) >>Visit HPA database.

Gene(ID):

RCAN1(1827)

Expression:

P53805 RCAN1_HUMAN:

Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues.

Sequence:

P53805 RCAN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEDGVAGPQLGAAAEAAEAAEARARPGVTLRPFAPLSGAAEADEGGGDWSFIDCEMEEVDLQDLPSATIACHLDPRVFVDGLCRAKFESLFRTYDKDITFQYFKSFKRVRINFSNPFSAADARLQLHKTEFLGKEMKLYFAQTLHIGSSHLAPPNPDKQFLISPPASPPVGWKQVEDATPVINYDLLYAISKLGPGEKYELHAATDTTPSVVVHVCESDQEKEEEEEMERMRRPKPKIIQTRRPEYTPIHLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53805 As Substrate

P53805

Site	PTM Type	Enzyme	Source
K86	Ubiquitination		Uniprot
Y94	Phosphorylation		Uniprot
K128	Ubiquitination		Uniprot
T129	Phosphorylation		Uniprot
K134	Ubiquitination		Uniprot
S163	Phosphorylation	Q99759 (MAP3K3) , P49841 (GSK3B)	Uniprot
S167	Phosphorylation	Q99759 (MAP3K3) , Q13627 (DYRK1A) , P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
T179	Phosphorylation		Uniprot
Y188	Phosphorylation		Uniprot
K192	Ubiquitination		Uniprot
T208	Phosphorylation		Uniprot
S218	Phosphorylation		Uniprot
T241	Phosphorylation		Uniprot
T247	Phosphorylation	Q13627 (DYRK1A)	Uniprot

Research Backgrounds

P53805_RCAN1_HUMAN

Function:

Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development (By similarity).

PTMs:

Phosphorylation increases its ability to inhibit calcineurin and decreases protein half-life.

Tissue Specificity:

Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues.

Subunit Structure:

Interacts with RAF1. Interacts with PPP3CA and PPP3R1.

Family&Domains:

Belongs to the RCAN family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). Mangiferin Inhibits PDGF-BB-Induced Proliferation and Migration of Rat Vascular Smooth Muscle Cells and Alleviates Neointimal Formation in Mice through the AMPK/Drp1 Axis. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34900084)

Application: WB Species: Mouse Sample:

Figure 4 Mangiferin alleviated PDGF-BB-induced mitochondrial fission and increase in ROS levels. (a) RSMCs were stained with mitotracker probe after treatment with different concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. The average length of mitochondria was quantified using ImageJ software. (b) Intracellular ROS levels were detected using flow cytometry after RSMCs were treated with mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. (c–e) Western blot was used to measure the protein expression levels of AMPK, pAMPKThr172, Drp1, and pDrp1Ser616 after RSMCs were treated with various concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. All assays were conducted in triplicate and all data presented as mean ± S.D. ∗P < 0.05, compared with the control group. #P < 0.05, compared with the PDGF-BB group.

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Phospho-Calcipressin 1 (Ser163) Antibody - #AF8268