Phospho-CUTL1 (Ser1215) Antibody - #AF8262

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Product: Phospho-CUTL1 (Ser1215) Antibody
Catalog: AF8262
Description: Rabbit polyclonal antibody to Phospho-CUTL1 (Ser1215)
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Sheep, Dog, Xenopus
Mol.Wt.: 120kD,160kD,200kD; 164kD(Calculated).
Uniprot: P39880
RRID: AB_2840324

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-CUTL1 (Ser1215) Antibody detects endogenous levels of CUTL1 only when phosphorylated at Ser1215.
RRID:
AB_2840324
Cite Format: Affinity Biosciences Cat# AF8262, RRID:AB_2840324.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CASP; CCAAT displacement protein; CDP; CDP/Cut; CDP1; Clox; COY1; Cut homeobox; Cut homolog; Cut like 1; Cut like homeobox 1; CUTL1; CUX; Cux/CDP; CUX1; Golgi integral membrane protein 6; GOLIM6; Homeobox protein cux 1; Nbla10317; p100; p110; p200; p75; Protein CASP; Putative protein product of Nbla10317;

Immunogens

Immunogen:
Uniprot:

P39880(CUX1_HUMAN) >>Visit HPA database.

Gene(ID):
CUX1(1523)
Sequence:
MLCVAGARLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPSEGAGTQDAAKPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPESRRPGSLPAPPPSQLPRNPGEQASNTNGTHQFSPAGLSQDFFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEMDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASGSGNSDDAIRSILQQARREMEAQQAALDPALKQAPLSQSDITILTPKLLSTSPMPTVSSYPPLAISLKKPSAAPEAGASALPNPPALKKEAQDAPGLDPQGAADCAQGVLRQVKNEVGRSGAWKDHWWSAVQPERRNAASSEEAKAEETGGGKEKGSGGSGGGSQPRAERSQLQGPSSSEYWKEWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPMSKPTKPSVPPLTPEQYEVYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIEASKDSKPPEPSDPPASDSQPTTPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGTEYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEDLATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEATEGPGSADTEEPKSQGEAEREEVPRPAEQTEPPPSGTPGPDDARDDDHEGGPVEGPGPLPSPASATATAAPAAPEDAATSAAAAPGEGPAAPSSAPPPSNSSSSSAPRRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Xenopus
100
Horse
0
Bovine
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P39880 As Substrate

Site PTM Type Enzyme
S59 Phosphorylation
K83 Ubiquitination
K117 Ubiquitination
K206 Ubiquitination
S322 Phosphorylation
Y351 Phosphorylation
S363 Phosphorylation
S369 Phosphorylation
T374 Phosphorylation
K388 Ubiquitination
S407 Phosphorylation
S409 Phosphorylation
S425 Phosphorylation
S432 Phosphorylation
S452 Phosphorylation
S467 Phosphorylation
K561 Ubiquitination
S585 Phosphorylation
T600 Phosphorylation
K604 Acetylation
K611 Ubiquitination
K647 Ubiquitination
S652 Phosphorylation
S663 Phosphorylation
T665 Phosphorylation
S667 Phosphorylation
K672 Ubiquitination
K679 Ubiquitination
K686 Ubiquitination
K729 Ubiquitination
S734 Phosphorylation
T739 Phosphorylation
T742 Phosphorylation
S755 Phosphorylation
S756 Phosphorylation
S763 Phosphorylation
S768 Phosphorylation
K811 Sumoylation
S817 Phosphorylation
K850 Acetylation
S868 Phosphorylation
S874 Phosphorylation
S887 Phosphorylation
Y889 Phosphorylation
S901 Phosphorylation
S909 Phosphorylation
S974 Phosphorylation
K990 Ubiquitination
S1054 Phosphorylation
S1059 Phosphorylation
S1069 Phosphorylation
T1071 Phosphorylation
S1100 Phosphorylation
T1103 Phosphorylation
T1104 Phosphorylation
S1115 Phosphorylation
T1157 Phosphorylation
S1215 Phosphorylation P17612 (PRKACA)
S1216 Phosphorylation
S1218 Phosphorylation
T1230 Phosphorylation
Y1232 Phosphorylation
S1233 Phosphorylation
S1237 Phosphorylation P17612 (PRKACA) , P06493 (CDK1)
K1256 Ubiquitination
S1270 Phosphorylation P06493 (CDK1) , P17612 (PRKACA)
S1312 Phosphorylation
S1321 Phosphorylation
S1333 Phosphorylation
S1337 Phosphorylation
T1352 Phosphorylation
S1357 Phosphorylation Q13535 (ATR)
T1380 Phosphorylation
S1404 Phosphorylation
S1454 Phosphorylation
S1455 Phosphorylation
S1458 Phosphorylation
S1486 Phosphorylation
S1496 Phosphorylation

Research Backgrounds

Function:

Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. Also involved in the control of synaptogenesis. In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with BANP. Interacts with SATB1 (via DNA-binding domains); the interaction inhibits the attachment of both proteins to DNA (By similarity).

Family&Domains:

Belongs to the CUT homeobox family.

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