Phospho-BORA (Ser497) Antibody - #AF8249
(1)
| Product: | Phospho-BORA (Ser497) Antibody |
| Catalog: | AF8249 |
| Description: | Rabbit polyclonal antibody to Phospho-BORA (Ser497) |
| Application: | WB |
| Reactivity: | Human, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| Mol.Wt.: | 61kDa; 61kD(Calculated). |
| Uniprot: | Q6PGQ7 |
| RRID: | AB_2840311 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Chicken(89%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
Phospho-BORA (Ser497) Antibody detects endogenous levels of BORA only when phosphorylated at Ser497.
RRID:
AB_2840311
Cite Format: Affinity Biosciences Cat# AF8249, RRID:AB_2840311.
Cite Format: Affinity Biosciences Cat# AF8249, RRID:AB_2840311.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Aurora borealis; bora; bora, aurora kinase A activator; BORA_HUMAN; C13orf34; Chromosome 13 open reading frame 34; FLJ22624; HsBora; Protein aurora borealis; RP11-342J4.2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q6PGQ7 BORA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDVKESKMQITPETPGRIPVLNPFESPSDYSNLHEQTLASPSVFKSTKLPTPGKFRWSIDQLAVINPVEIDPEDIHRQALYLSHSRIDKDVEDKRQKAIEEFFTKDVIVPSPWTDHEGKQLSQCHSSKCTNINSDSPVGKKLTIHSEKSDAACQTLLSLPVDFNLENILGDYFRADEFADQSPGNLSSSSLRRKLFLDGNGSISDSLPSASPGSPHSGVQTSLEMFYSIDLSPVKCRSPLQTPSSGQFSSSPIQASAKKYSLGSITSPSPISSPTFSPIEFQIGETPLSEQRKFTVHSPDASSGTNSNGITNPCIRSPYIDGCSPIKNWSPMRLQMYSGGTQYRTSVIQIPFTLETQGEDEEDKENIPSTDVSSPAMDAAGIHLRQFSNEASTHGTHLVVTAMSVTQNQSSASEKELALLQDVEREKDNNTVDMVDPIEIADETTWIKEPVDNGSLPMTDFVSGIAFSIENSHMCMSPLAESSVIPCESSNIQMDSGYNTQNCGSNIMDTVGAESYCKESDAQTCEVESKSQAFNMKQDHTTQRCWMKTASPFQCSSP
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
89
Chicken
89
Rabbit
89
Zebrafish
78
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6PGQ7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y31 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| K98 | Acetylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S270 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S278 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| T296 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | P50750 (CDK9) | Uniprot |
| S331 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| T445 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T501 | Phosphorylation | Q13535 (ATR) , P53350 (PLK1) | Uniprot |
| K531 | Ubiquitination | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Required for the activation of AURKA at the onset of mitosis.
PTMs:
Phosphorylated by AURKA.
Subunit Structure:
Interacts with AURKA.
Family&Domains:
Belongs to the BORA family.
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For Research Use Only.
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