Phospho-BAG3 (Tyr457) Antibody - #AF8243

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Product: Phospho-BAG3 (Tyr457) Antibody
Catalog: AF8243
Description: Rabbit polyclonal antibody to Phospho-BAG3 (Tyr457)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 62 KD; 62kD(Calculated).
Uniprot: O95817
RRID: AB_2840305

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-BAG3 (Tyr457) Antibody detects endogenous levels of BAG3 only when phosphorylated at Tyr457.
RRID:
AB_2840305
Cite Format: Affinity Biosciences Cat# AF8243, RRID:AB_2840305.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAG 3; BAG family molecular chaperone regulator 3; BAG-3; Bag3; BAG3_HUMAN; Bcl 2 binding protein; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; BCL2 associated athanogene 3; BCL2 binding athanogene 3; BIS; CAIR 1; Docking protein CAIR 1; Docking protein CAIR-1; MFM6;

Immunogens

Immunogen:
Uniprot:

O95817(BAG3_HUMAN) >>Visit HPA database.

Gene(ID):
BAG3(9531)
Sequence:
MSAATHSPMMQVASGNGDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPSEGPKETPSSANGPSREGSRLPPAREGHPVYPQLRPGYIPIPVLHEGAENRQVHPFHVYPQPGMQRFRTEAAAAAPQRSQSPLRGMPETTQPDKQCGQVAAAAAAQPPASHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQAQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRETAPVSQPENKPESKPGPVGPELPPGHIPIQVIRKEVDSKPVSQKPPPPSEKVEVKVPPAPVPCPPPSPGPSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
67
Zebrafish
67
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95817 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S7 Phosphorylation
K29 Acetylation
K60 Ubiquitination
Y86 Phosphorylation
R90 Methylation
Y93 Phosphorylation
R106 Methylation
Y114 Phosphorylation
R121 Methylation
R123 Methylation
R133 Methylation
S134 Phosphorylation
S136 Phosphorylation
R139 Methylation
K149 Ubiquitination
C151 S-Nitrosylation
S171 Phosphorylation
S173 Phosphorylation
S177 Phosphorylation
C179 S-Nitrosylation
S180 Phosphorylation
S187 Phosphorylation Q05655 (PRKCD)
S190 Phosphorylation
S194 Phosphorylation
S195 Phosphorylation
S198 Phosphorylation
R203 Methylation
Y205 Phosphorylation
S207 Phosphorylation
R218 Methylation
S224 Phosphorylation
K230 Methylation
K230 Ubiquitination
Y233 Phosphorylation
Y240 Phosphorylation
T242 Phosphorylation
Y247 Phosphorylation
K249 Ubiquitination
R258 Methylation
R261 Methylation
S264 Phosphorylation
R267 Methylation
S268 Phosphorylation
S269 Phosphorylation
S274 Phosphorylation
S275 Phosphorylation
S279 Phosphorylation
S283 Phosphorylation
S284 Phosphorylation
T285 Phosphorylation P42345 (MTOR)
S289 Phosphorylation P42345 (MTOR)
S291 Phosphorylation
S315 Phosphorylation
K344 Ubiquitination
S377 Phosphorylation
S381 Phosphorylation
S385 Phosphorylation
S386 Phosphorylation
S389 Phosphorylation
T392 Phosphorylation
T406 Phosphorylation
K445 Acetylation
K445 Sumoylation
K445 Ubiquitination
K450 Ubiquitination
Y451 Phosphorylation
Y457 Phosphorylation
T459 Phosphorylation
K493 Ubiquitination
Y508 Phosphorylation

Research Backgrounds

Function:

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Colocalizes with HSF1 to the nucleus upon heat stress (PubMed:26159920).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds to the ATPase domain of HSP70/HSC70 chaperones. Interacts with BCL2. Interacts with phospholipase C-gamma proteins. Interacts with DNAJB6. Interacts (via BAG domain) with HSF1; this interaction occurs in normal and heat-shocked cells promoting HSF1 nucleocytoplasmic shuttling. Interacts with HSPA8 (via NBD). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD). Interacts (via WW domain 1) with SYNPO2 (via PPPY motif). Interacts with HSPB8.

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