Phospho-Acinus (Ser1180) Antibody - #AF8237

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Product: Phospho-Acinus (Ser1180) Antibody
Catalog: AF8237
Description: Rabbit polyclonal antibody to Phospho-Acinus (Ser1180)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 150KD; 152kD,126kD,93kD,59kD(Calculated).
Uniprot: Q9UKV3 | O95104 | Q8TF01 | Q14498
RRID: AB_2840299

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Acinus (Ser1180) Antibody detects endogenous levels of Acinus only when phosphorylated at Ser1180.
RRID:
AB_2840299
Cite Format: Affinity Biosciences Cat# AF8237, RRID:AB_2840299.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACIN 1; ACIN1; ACINU_HUMAN; Acinus; ACN; Apoptotic chromatin condensation inducer 1; Apoptotic chromatin condensation inducer in the nucleus; DKFZp667N107; fSAP152; functional spliceosome-associated protein 152; KIAA0670; arginine/serine-rich 15; CTD-binding SR-like protein RA4; DKFZp434E098; FLJ23364; KIAA1172; Pre mRNA splicing SR protein rA4; SCAF4; SFR15_HUMAN; Splicing factor; Splicing factor arginine/serine rich 15; Splicing factor serine alanine 15; SR like CTD associated factor 4; SR-related and CTD-associated factor 4; SRA4; CAPER alpha; CAPER; CAPERalpha; CC1.3; Coactivator of activating protein 1 and estrogen receptors; Coactivator of AP1 and ERs; DKFZp781C0423; FLJ44170; HCC 1; Hepatocellular carcinoma protein 1; RBM 39; RBM39; RBM39_HUMAN; RNA binding motif protein 39; RNA binding region (RNP1 RRM) containing 2; RNA binding region containing 2; RNA binding region containing protein 2; RNA-binding motif protein 39; RNA-binding protein 39; RNA-binding region-containing protein 2; RNPC 2; RNPC2; Splicing factor CC1.3; Splicing factor CC1.4; Splicing factor HCC 1; Splicing factor HCC1; Transcription coactivator CAPER;

Immunogens

Immunogen:
Uniprot:

Q9UKV3(ACINU_HUMAN) >>Visit HPA database.

O95104(SCAF4_HUMAN) >>Visit HPA database.

Q8TF01(PNISR_HUMAN) >>Visit HPA database.

Q14498(RBM39_HUMAN) >>Visit HPA database.

Gene(ID):
SCAF4(57466) | RBM39(9584) | PNISR(25957) | ACIN1(22985)
Expression:
Q9UKV3 ACINU_HUMAN:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

Q8TF01 PNISR_HUMAN:

Expressed in heart, skeletal muscle, thymus, spleen, kidney, liver, placenta and leukocytes.

Q14498 RBM39_HUMAN:

Widely expressed. Highly expressed in pancreas, skeletal muscle, lung and brain. Expressed at intermediate level in kidney, liver and heart.

Sequence:
MWRRKHPRTSGGTRGVLSGNRGVEYGSGRGHLGTFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGKPLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQYLEKQQELLRQRLEREAREAAELEEASAESEDEMIHPEGVASLLPPDFQSSLERPELELSRHSPRKSSSISEEKGDSDDEKPRKGERRSSRVRQARAAKLSEGSQPAEEEEDQETPSRNLRVRADRNLKTEEEEEEEEEEEEDDEEEEGDDEGQKSREAPILKEFKEEGEEIPRVKPEEMMDERPKTRSQEQEVLERGGRFTRSQEEARKSHLARQQQEKEMKTTSPLEEEEREIKSSQGLKEKSKSPSPPRLTEDRKKASLVALPEQTASEEETPPPLLTKEASSPPPHPQLHSEEEIEPMEGPAPAVLIQLSPPNTDADTRELLVSQHTVQLVGGLSPLSSPSDTKAESPAEKVPEESVLPLVQKSTLADYSAQKDLEPESDRSAQPLPLKIEELALAKGITEECLKQPSLEQKEGRRASHTLLPSHRLKQSADSSSSRSSSSSSSSSRSRSRSPDSSGSRSHSPLRSKQRDVAQARTHANPRGRPKMGSRSTSESRSRSRSRSRSASSNSRKSLSPGVSRDSSTSYTETKDPSSGQEVATPPVPQLQVCEPKERTSTSSSSVQARRLSQPESAEKHVTQRLQPERGSPKKCEAEEAEPPAATQPQTSETQTSHLPESERIHHTVEEKEEVTMDTSENRPENDVPEPPMPIADQVSNDDRPEGSVEDEEKKESSLPKSFKRKISVVSATKGVPAGNSDTEGGQPGRKRRWGASTATTQKKPSISITTESLKSLIPDIKPLAGQEAVVDLHADDSRISEDETERNGDDGTHDKGLKICRTVTQVVPAEGQENGQREEEEEEKEPEAEPPVPPQVSVEVALPPPAEHEVKKVTLGDTLTRRSISQQKSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKTEEQGIPRPLHPPPPPPVQPPQHPRAEQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTDSQIVQKEAERAERAKEREKRRKEQEEEEQKEREKEAERERNRQLEREKRREHSRERDRERERERERDRGDRDRDRERDRERGRERDRRDTKRHSRSRSRSTPVRDRGGRR

MDAVNAFNQELFSLMDMKPPISRAKMILITKAAIKAIKLYKHVVQIVEKFIKKCKPEYKVPGLYVIDSIVRQSRHQFGTDKDVFGPRFSKNITATFQYLYLCPSEDKSKIVRVLNLWQKNGVFKIEIIQPLLDMAAGTSNAAPVAENVTNNEGSPPPPVKVSSEPPTQATPNSVPAVPQLPSSDAFAAVAQLFQTTQGQQLQQILQTFQQPPKPQSPALDNAVMAQVQAITAQLKTTPTQPSEQKAAFPPPEQKTAFDKKLLDRFDYDDEPEAVEESKKEDTTAVTTTAPAAAVPPAPTATVPAAAAPAAASPPPPQAPFGFPGDGMQQPAYTQHQNMDQFQPRMMGIQQDPMHHQVPLPPNGQMPGFGLLPTPPFPPMAQPVIPPTPPVQQPFQASFQAQNEPLTQKPHQQEMEVEQPCIQEVKRHMSDNRKSRSRSASRSPKRRRSRSGSRSRRSRHRRSRSRSRDRRRHSPRSRSQERRDREKERERRQKGLPQVKPETASVCSTTLWVGQLDKRTTQQDVASLLEEFGPIESINMIPPRGCAYIVMVHRQDAYRALQKLSRGNYKVNQKSIKIAWALNKGIKADYKQYWDVELGVTYIPWDKVKPEELESFCEGGMLDSDTLNPDWKGIPKKPENEVAQNGGAETSHTEPVSPIPKPLPVPVPPIPVPAPITVPPPQVPPHQPGPPVVGALQPPAFTPPLGIPPPGFGPGVPPPPPPPPFLRPGFNPMHLPPGFLPPGPPPPITPPVSIPPPHTPPISIPNSTIAGINEDTTKDLSIGNPIPTVVSGARGNAESGDSVKMYGSAVPPAAPTNLPTPPVTQPVSLLGTQGVAPGPVIGLQAPSTGLLGARPGLIPLQRPPGMPPPHLQRFPLMPPRPMPPHMMHRGPPPGPGGFAMPPPHGMKGPFPPHGPFVRPGGMPGLGGPGPGPGGPEDRDGRQQPPQQPQQQPQPQAPQQPQQQQQQQPPPSQQPPPTQQQPQQFRNDNRQQFNSGRDQERFGRRSFGNRVENDRERYGNRNDDRDNSNRDRREWGRRSPDRDRHRDLEERNRRSSGHRDRERDSRDRESRREKEEARGKEKPEVTDRAGGNKTVEPPISQVGNVDTASELEKGVSEAAVLKPSEELPAEATSSVEPEKDSGSAAEAPR

MWDQGGQPWQQWPLNQQQWMQSFQHQQDPSQIDWAALAQAWIAQREASGQQSMVEQPPGMMPNGQDMSTMESGPNNHGNFQGDSNFNRMWQPEWGMHQQPPHPPPDQPWMPPTPGPMDIVPPSEDSNSQDSGEFAPDNRHIFNQNNHNFGGPPDNFAVGPVNQFDYQHGAAFGPPQGGFHPPYWQPGPPGPPAPPQNRRERPSSFRDRQRSPIALPVKQEPPQIDAVKRRTLPAWIREGLEKMEREKQKKLEKERMEQQRSQLSKKEKKATEDAEGGDGPRLPQRSKFDSDEEEEDTENVEAASSGKVTRSPSPVPQEEHSDPEMTEEEKEYQMMLLTKMLLTEILLDVTDEEIYYVAKDAHRKATKAPAKQLAQSSALASLTGLGGLGGYGSGDSEDERSDRGSESSDTDDEELRHRIRQKQEAFWRKEKEQQLLHDKQMEEEKQQTERVTKEMNEFIHKEQNSLSLLEAREADGDVVNEKKRTPNETTSVLEPKKEHKEKEKQGRSRSGSSSSGSSSSNSRTSSTSSTVSSSSYSSSSGSSRTSSRSSSPKRKKRHSRSRSPTIKARRSRSRSYSRRIKIESNRARVKIRDRRRSNRNSIERERRRNRSPSRERRRSRSRSRDRRTNRASRSRSRDRRKIDDQRGNLSGNSHKHKGEAKEQERKKERSRSIDKDRKKKDKEREREQDKRKEKQKREEKDFKFSSQDDRLKRKRESERTFSRSGSISVKIIRHDSRQDSKKSTTKDSKKHSGSDSSGRSSSESPGSSKEKKAKKPKHSRSRSVEKSQRSGKKASRKHKSKSRSR

MADDIDIEAMLEAPYKKDENKLSSANGHEERSKKRKKSKSRSRSHERKRSKSKERKRSRDRERKKSKSRERKRSRSKERRRSRSRSRDRRFRGRYRSPYSGPKFNSAIRGKIGLPHSIKLSRRRSRSKSPFRKDKSPVREPIDNLTPEERDARTVFCMQLAARIRPRDLEEFFSTVGKVRDVRMISDRNSRRSKGIAYVEFVDVSSVPLAIGLTGQRVLGVPIIVQASQAEKNRAAAMANNLQKGSAGPMRLYVGSLHFNITEDMLRGIFEPFGRIESIQLMMDSETGRSKGYGFITFSDSECAKKALEQLNGFELAGRPMKVGHVTERTDASSASSFLDSDELERTGIDLGTTGRLQLMARLAEGTGLQIPPAAQQALQMSGSLAFGAVAEFSFVIDLQTRLSQQTEASALAAAASVQPLATQCFQLSNMFNPQTEEEVGWDTEIKDDVIEECNKHGGVIHIYVDKNSAQGNVYVKCPSIAAAIAAVNALHGRWFAGKMITAAYVPLPTYHNLFPDSMTATQLLVPSRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
43
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKV3/O95104/Q8TF01/Q14498 As Substrate

Site PTM Type Enzyme
K49 Acetylation
S73 Phosphorylation
K119 Ubiquitination
T149 Phosphorylation
S154 Phosphorylation
T236 Phosphorylation
T237 Phosphorylation
Y267 Phosphorylation
S464 Phosphorylation
S466 Phosphorylation
S473 Phosphorylation
S476 Phosphorylation
K576 Ubiquitination
K583 Ubiquitination
K631 Ubiquitination
T652 Phosphorylation
S656 Phosphorylation
R888 Methylation
R917 Methylation
R937 Methylation
R940 Methylation
S1004 Phosphorylation
S1037 Phosphorylation
S1054 Phosphorylation
S1068 Phosphorylation
R1086 Methylation
S1098 Phosphorylation
S1131 Phosphorylation
S1141 Phosphorylation
Site PTM Type Enzyme
K5 Ubiquitination
R8 Methylation
R14 Methylation
S18 Phosphorylation
R29 Methylation
K81 Methylation
K81 Ubiquitination
R87 Methylation
S96 Phosphorylation
K103 Methylation
K103 Ubiquitination
K113 Methylation
S115 Phosphorylation
T116 Phosphorylation
S125 Phosphorylation
S132 Phosphorylation
S135 Phosphorylation
K138 Ubiquitination
K143 Methylation
K143 Ubiquitination
S166 Phosphorylation
S169 Phosphorylation
S189 Phosphorylation
S190 Phosphorylation
S199 Phosphorylation
S206 Phosphorylation
S208 Phosphorylation
S210 Phosphorylation
S216 Phosphorylation
S228 Phosphorylation
S229 Phosphorylation
K238 Acetylation
S240 Phosphorylation
S243 Phosphorylation
T254 Phosphorylation
S256 Phosphorylation
K268 Sumoylation
T269 Phosphorylation
S295 Phosphorylation
K302 Ubiquitination
K315 Sumoylation
T326 Phosphorylation
S328 Phosphorylation
R336 Methylation
T341 Phosphorylation
S343 Phosphorylation
K359 Acetylation
T363 Phosphorylation
T364 Phosphorylation
S365 Phosphorylation
S376 Phosphorylation
S377 Phosphorylation
S384 Phosphorylation
S386 Phosphorylation
S388 Phosphorylation
T393 Phosphorylation
S400 Phosphorylation
T408 Phosphorylation
S410 Phosphorylation
T414 Phosphorylation
T420 Phosphorylation
S424 Phosphorylation
S425 Phosphorylation
S434 Phosphorylation
S453 Phosphorylation
T457 Phosphorylation
T461 Phosphorylation
S478 Phosphorylation
S481 Phosphorylation
S482 Phosphorylation
S484 Phosphorylation
T486 Phosphorylation
S490 Phosphorylation
S499 Phosphorylation
K506 Ubiquitination
Y512 Phosphorylation
S513 Phosphorylation
K516 Ubiquitination
S522 Phosphorylation
R524 Methylation
S525 Phosphorylation
K532 Sumoylation
K540 Ubiquitination
K548 Ubiquitination
S551 Phosphorylation
S561 Phosphorylation
T563 Phosphorylation
S583 Phosphorylation
S584 Phosphorylation
S585 Phosphorylation
S586 Phosphorylation
S588 Phosphorylation
S595 Phosphorylation
S599 Phosphorylation
S601 Phosphorylation
S605 Phosphorylation
S635 Phosphorylation
S637 Phosphorylation
S645 Phosphorylation
K654 Methylation
S655 Phosphorylation
S657 Phosphorylation
S661 Phosphorylation
S664 Phosphorylation
T666 Phosphorylation
Y668 Phosphorylation
T671 Phosphorylation
S675 Phosphorylation
S676 Phosphorylation
T682 Phosphorylation
C691 S-Nitrosylation
T697 Phosphorylation
S698 Phosphorylation
T699 Phosphorylation
S702 Phosphorylation
S703 Phosphorylation
S710 Phosphorylation
S714 Phosphorylation
K717 Acetylation
T720 Phosphorylation
S729 Phosphorylation
T744 Phosphorylation
T748 Phosphorylation
T751 Phosphorylation
S754 Phosphorylation
S814 Phosphorylation
S815 Phosphorylation
K818 Ubiquitination
S819 Phosphorylation
K823 Ubiquitination
S825 Phosphorylation
S828 Phosphorylation
T830 Phosphorylation
K831 Ubiquitination
S838 Phosphorylation
T840 Phosphorylation
K860 Ubiquitination
K861 Ubiquitination
S863 Phosphorylation
S865 Phosphorylation
S895 Phosphorylation
S898 Phosphorylation
T902 Phosphorylation
T976 Phosphorylation
T978 Phosphorylation
S981 Phosphorylation
S983 Phosphorylation
K986 Ubiquitination
S987 Phosphorylation
S990 Phosphorylation
T992 Phosphorylation
T999 Phosphorylation
S1004 Phosphorylation
K1029 Ubiquitination
K1047 Ubiquitination
S1104 Phosphorylation
T1106 Phosphorylation
S1161 Phosphorylation
S1176 Phosphorylation
S1178 Phosphorylation
S1180 Phosphorylation P78362 (SRPK2) , P31749 (AKT1)
K1209 Ubiquitination
K1219 Ubiquitination
Y1225 Phosphorylation
T1230 Phosphorylation
S1232 Phosphorylation
K1237 Ubiquitination
K1253 Methylation
S1284 Phosphorylation
S1325 Phosphorylation
S1327 Phosphorylation
S1329 Phosphorylation P31749 (AKT1)
S1331 Phosphorylation
T1332 Phosphorylation
Site PTM Type Enzyme
S204 Phosphorylation
S211 Phosphorylation
K218 Sumoylation
K228 Ubiquitination
K266 Acetylation
T271 Phosphorylation
S286 Phosphorylation
S290 Phosphorylation
S304 Phosphorylation
S305 Phosphorylation
T309 Phosphorylation
S311 Phosphorylation
S313 Phosphorylation
S321 Phosphorylation
T326 Phosphorylation
S393 Phosphorylation
S396 Phosphorylation
S401 Phosphorylation
S405 Phosphorylation
S407 Phosphorylation
S408 Phosphorylation
T410 Phosphorylation
S465 Phosphorylation
S467 Phosphorylation
T485 Phosphorylation
T489 Phosphorylation
S517 Phosphorylation
S518 Phosphorylation
S522 Phosphorylation
T524 Phosphorylation
T527 Phosphorylation
S529 Phosphorylation
T530 Phosphorylation
S532 Phosphorylation
S533 Phosphorylation
S537 Phosphorylation
S539 Phosphorylation
S540 Phosphorylation
S561 Phosphorylation
S563 Phosphorylation
S571 Phosphorylation
S573 Phosphorylation
S575 Phosphorylation
S577 Phosphorylation
S597 Phosphorylation
S601 Phosphorylation
S611 Phosphorylation
S613 Phosphorylation
S621 Phosphorylation
S623 Phosphorylation
S634 Phosphorylation
S636 Phosphorylation
S650 Phosphorylation
S672 Phosphorylation
S705 Phosphorylation
S706 Phosphorylation
S722 Phosphorylation
S724 Phosphorylation
S726 Phosphorylation
K730 Acetylation
S752 Phosphorylation
Site PTM Type Enzyme
A2 Acetylation
Y15 Phosphorylation
K16 Sumoylation
K21 Sumoylation
S23 Phosphorylation
S24 Phosphorylation
S40 Phosphorylation
S44 Phosphorylation
S50 Phosphorylation
S58 Phosphorylation
S84 Phosphorylation
S86 Phosphorylation
Y95 Phosphorylation P00519 (ABL1)
R96 Methylation
S97 Phosphorylation
Y99 Phosphorylation P00519 (ABL1)
S100 Phosphorylation
K103 Acetylation
S106 Phosphorylation
R109 Methylation
K111 Methylation
S117 Phosphorylation
K119 Sumoylation
K119 Ubiquitination
S121 Phosphorylation
R122 Methylation
R123 Methylation
R124 Methylation
S125 Phosphorylation
S127 Phosphorylation
S129 Phosphorylation
S136 Phosphorylation
T146 Phosphorylation
T154 Phosphorylation
S174 Phosphorylation
K178 Ubiquitination
R183 Methylation
S228 Phosphorylation
K232 Sumoylation
K232 Ubiquitination
K244 Ubiquitination
Y253 Phosphorylation
K306 Ubiquitination
K322 Ubiquitination
S333 Phosphorylation
S334 Phosphorylation
S336 Phosphorylation
S337 Phosphorylation
S341 Phosphorylation
R346 Methylation
T354 Phosphorylation
Y475 Phosphorylation
K477 Ubiquitination
C478 S-Nitrosylation
Y505 Phosphorylation

Research Backgrounds

Function:

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

PTMs:

Phosphorylation on Ser-1180 by SRPK2 up-regulates its stimulatory effect on cyclin A1.

Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.

Subcellular Location:

Nucleus. Nucleus speckle. Nucleus>Nucleoplasm.
Note: Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

Subunit Structure:

Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) complexes consisting of RNPS1, SAP18 and different isoforms of ACIN1; the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Interacts with API5. Interacts with SRPK2 in a phosphorylation-dependent manner.

Function:

Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF8, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF8, also acts as a suppressor of transcriptional readthrough.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with POLR2A; via C-terminal heptapeptide repeat domain (CTD) phosphorylated at 'Ser-2' and 'Ser-5'.

Subcellular Location:

Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, skeletal muscle, thymus, spleen, kidney, liver, placenta and leukocytes.

Subunit Structure:

Interacts with PNN.

Family&Domains:

Belongs to the splicing factor SR family.

Function:

Transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1 (By similarity). May be involved in pre-mRNA splicing process.

Subcellular Location:

Nucleus speckle.
Note: Concentrated in nuclear speckles. Colocalizes with the core spliceosomal snRNP proteins.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highly expressed in pancreas, skeletal muscle, lung and brain. Expressed at intermediate level in kidney, liver and heart.

Subunit Structure:

Interacts with NCOA6 and JUN. Interacts with ESR1 and ESR2, in the presence of estradiol (E2) (By similarity). Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with SF3B1. Interacts with ZNF106 (via N-terminus) (By similarity).

Family&Domains:

Belongs to the splicing factor SR family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Transcription > Spliceosome.

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