Phospho-nNOS (Ser1417) Antibody | Affinity Biosciences

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Figure 3 Changes in the NO/cGMP pathway within the corpus cavernosum region of the four groups.

Product:	Phospho-nNOS (Ser1417) Antibody
Catalog:	AF8231
Description:	Rabbit polyclonal antibody to Phospho-nNOS (Ser1417)
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	120~160kDa; 161kD(Calculated).
Uniprot:	P29475
RRID:	AB_2840293

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(91%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-nNOS (Ser1417) Antibody detects endogenous levels of nNOS only when phosphorylated at Ser1417.

RRID:

AB_2840293
Cite Format: Affinity Biosciences Cat# AF8231, RRID:AB_2840293.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2310005C01Rik; BNOS; Constitutive NOS; EC 1.14.13.39; IHPS 1; IHPS1; N-NOS; NC-NOS; neuronal Nitric Oxide Synthase; Neuronal NOS; Nitric oxide synthase , neuronal, included; Nitric oxide synthase 1 (neuronal); Nitric oxide synthase 1; Nitric oxide synthase, brain; Nitric oxide synthase, penile neuronal, included; NNOS; NO; NOS 1; NOS; NOS type I; NOS-I; NOS1; NOS1_HUMAN; Peptidyl-cysteine S-nitrosylase NOS1;

Immunogens

Immunogen:

Uniprot:

P29475(NOS1_HUMAN) >>Visit HPA database.

Gene(ID):

NOS1(4842)

Expression:

P29475 NOS1_HUMAN:

Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

Sequence:

P29475 NOS1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Bovine

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29475 As Substrate

P29475

Site	PTM Type	Enzyme	Source
S292	Phosphorylation		Uniprot
T395	Phosphorylation		Uniprot
T396	Phosphorylation		Uniprot
S397	Phosphorylation		Uniprot
Y453	Phosphorylation		Uniprot
Y609	Phosphorylation		Uniprot
T724	Phosphorylation		Uniprot
S746	Phosphorylation	Q14012 (CAMK1)	Uniprot
T766	Phosphorylation		Uniprot
T768	Phosphorylation		Uniprot
S852	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
S857	Phosphorylation		Uniprot
S859	Phosphorylation		Uniprot
S885	Phosphorylation		Uniprot
T1358	Phosphorylation		Uniprot
S1378	Phosphorylation		Uniprot
S1387	Phosphorylation		Uniprot
S1417	Phosphorylation		Uniprot

Research Backgrounds

P29475_NOS1_HUMAN

Function:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

PTMs:

Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).

Subcellular Location:

Cell membrane>Sarcolemma>Peripheral membrane protein. Cell projection>Dendritic spine.
Note: In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).

Tissue Specificity:

Subunit Structure:

Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity). Interacts with HTR4. Interacts with VAC14 (By similarity). Interacts with SLC6A4 (By similarity). Interacts (via N-terminal domain) with DLG4 (via N-terminal tandem pair of PDZ domains) (By similarity).

Family&Domains:

The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14 (By similarity).

Belongs to the NOS family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Salivary secretion.

References

1). Nitro-oleic acid ameliorates erectile dysfunction in a streptozotocin-induced rat model of diabetes by inhibiting oxidative stress and apoptosis and activating the NO/cGMP pathway. Asian Journal of Andrology, 2023 (PubMed: 37695220) [IF=2.9]

Application: IF/ICC Species: Rat Sample: corpus cavernosum tissue

Figure 3 Changes in the NO/cGMP pathway within the corpus cavernosum region of the four groups. (a) NO levels were determined using assay kits. Concentrations of (b) cGMP and (c) ADMA in the corpus cavernosum region as determined using ELISA. The expression levels of (d) p-eNOS and (e) p-nNOS were determined using immunofluorescence. Scale bars = 50 µm. Bar graph illustrating (f) eNOS and (g) nNOS concentrations in the corpus cavernosum region. n = 5 per group. All data are presented as the mean ± standard error of the mean. ***P < 0.001, ****P < 0.0001, the indicated group vs the control group; #P < 0.05, ##P < 0.01, the indicated group vs the DMED group. NO2-OA: nitro-oleic acid; DMED: diabetes mellitus erectile dysfunction; p-eNOS: phospho-endothelial nitric oxide synthase; p-nNOS: phospho-neuronal nitric oxide synthase; ADMA: asymmetric dimethylarginine; NO: nitric oxide; cGMP: 3’,5’-cyclic guanosine monophosphate; DAPI: 4’,6-diamidino-2-phenylindole; ELISA: enzyme-linked immunosorbent assay.

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Phospho-nNOS (Ser1417) Antibody - #AF8231