Phospho-EPH B1/3/4 (Tyr778/Tyr792/Tyr774) Antibody - #AF8222
| Product: | Phospho-EPH B1/3/4 (Tyr778/Tyr792/Tyr774) Antibody |
| Catalog: | AF8222 |
| Description: | Rabbit polyclonal antibody to Phospho-EPH B1/3/4 (Tyr778/Tyr792/Tyr774) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 110KD; 110kD,108kD(Calculated). |
| Uniprot: | P54762 | P54753 | P54760 |
| RRID: | AB_2840284 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8222, RRID:AB_2840284.
Fold/Unfold
Cek 6; EK6; ELK; Elkh; EPH receptor B1; Eph tyrosine kinase 2; EPH-like kinase 6; Ephb1; EPHB1_HUMAN; Ephrin type B receptor 1; Ephrin type-B receptor 1; EPHT2; HEK 6; HEK6; NET; Neuronally-expressed EPH-related tyrosine kinase; soluble EPHB1 variant 1; Tyrosine protein kinase receptor EPH 2; Tyrosine-protein kinase receptor EPH-2; Cek10; EK2; Embryonic kinase 2; EPH Like Tyrosine Kinase 2; EPH receptor B3; EPH-like kinase 2; ephb3; EPHB3_HUMAN; Ephrin receptor EphB3; Ephrin type B receptor 3; Ephrin type-B receptor 3; ETK2; hEK2; Human Embryo Kinase 2; Mdk5; Sek4; TYRO6; Tyrosine protein kinase receptor HEK2; Tyrosine protein kinase TYRO6; Tyrosine-protein kinase TYRO6; Ephb4; EPHB4_HUMAN; Ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma Transmembrane Kinase; HTK; MYK 1; MYK1; TYRO 11; TYRO11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11;
Immunogens
Preferentially expressed in brain.
P54753 EPHB3_HUMAN:Ubiquitous.
P54760 EPHB4_HUMAN:Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
- P54762 EPHB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALDYLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSMRVQISQSPTAMA
- P54753 EPHB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARARPPPPPSPPPGLLPLLPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLRLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSEGIASTVTSQMNSVQLDGLRPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPLIVGSATAGLVFVVAVVVIAIVCLRKQRHGSDSEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKQPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAQSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQV
- P54760 EPHB4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P54762/P54753/P54760 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T34 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| T544 | Phosphorylation | Uniprot | |
| Y574 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| Y581 | Phosphorylation | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| Y590 | Phosphorylation | Uniprot | |
| T595 | Phosphorylation | Uniprot | |
| Y596 | Phosphorylation | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| Y614 | Phosphorylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| T713 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| T773 | Phosphorylation | Uniprot | |
| Y774 | Phosphorylation | Uniprot | |
| T775 | Phosphorylation | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S777 | Phosphorylation | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| K885 | Ubiquitination | Uniprot | |
| Y906 | Phosphorylation | Uniprot | |
| S911 | Phosphorylation | Uniprot | |
| Y924 | Phosphorylation | Uniprot | |
| S943 | Phosphorylation | Uniprot | |
| T953 | Phosphorylation | Uniprot | |
| K960 | Ubiquitination | Uniprot | |
| S964 | Phosphorylation | Uniprot | |
| K973 | Ubiquitination | Uniprot | |
| T976 | Phosphorylation | Uniprot | |
| Y987 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T92 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| Y575 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| Y594 | Phosphorylation | Uniprot | |
| T599 | Phosphorylation | Uniprot | |
| Y600 | Phosphorylation | Uniprot | |
| Y634 | Phosphorylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| Y740 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S761 | Phosphorylation | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| Y768 | Phosphorylation | Uniprot | |
| S774 | Phosphorylation | Uniprot | |
| T777 | Phosphorylation | Uniprot | |
| Y778 | Phosphorylation | Uniprot | |
| Y798 | Phosphorylation | Uniprot | |
| Y928 | Phosphorylation | Uniprot | |
| S968 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S167 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| Y593 | Phosphorylation | Uniprot | |
| T594 | Phosphorylation | Uniprot | |
| Y600 | Phosphorylation | Uniprot | |
| Y608 | Phosphorylation | Uniprot | |
| T613 | Phosphorylation | Uniprot | |
| Y614 | Phosphorylation | P54753 (EPHB3) | Uniprot |
| T666 | Phosphorylation | Uniprot | |
| T731 | Phosphorylation | Uniprot | |
| Y754 | Phosphorylation | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| S775 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| T791 | Phosphorylation | Uniprot | |
| Y792 | Phosphorylation | Uniprot | |
| S794 | Phosphorylation | Uniprot | |
| S795 | Phosphorylation | Uniprot | |
| Y812 | Phosphorylation | Uniprot | |
| T891 | Phosphorylation | Uniprot | |
| S901 | Phosphorylation | Uniprot | |
| Y924 | Phosphorylation | Uniprot | |
| T971 | Phosphorylation | Uniprot |
PTMs - P54762/P54753/P54760 As Enzyme
Research Backgrounds
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (By similarity).
Phosphorylated. Autophosphorylation is stimulated by the ligand EFNB1. Required for interaction with SH2 domain-containing interactors, for activation of the MAPK/ERK and JUN signaling cascades and for ubiquitination by CBL.
Ubiquitinated; (EFNB1)ligand-induced poly- and/or multi-ubiquitination by CBL is regulated by SRC and leads to lysosomal degradation.
Cell membrane>Single-pass type I membrane protein. Early endosome membrane. Cell projection>Dendrite.
Preferentially expressed in brain.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1 (By similarity). Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion (By similarity). The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (residues within the catalytic domain) with GRB2 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-614 is required for interaction with SH2 domain-containing proteins.
Cell membrane>Single-pass type I membrane protein. Cell projection>Dendrite.
Ubiquitous.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
Phosphorylated; autophosphorylation is stimulated by EFNB2.
Cell membrane>Single-pass type I membrane protein.
Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts with RASA1; the interaction depends on EPHB4 tyrosine-phosphorylation.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Research Fields
· Organismal Systems > Development > Axon guidance. (View pathway)
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