ADPGK Antibody | Affinity Biosciences

IF/ICC

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Product:	ADPGK Antibody
Catalog:	AF0517
Description:	Rabbit polyclonal antibody to ADPGK
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	47kDa; 54kD(Calculated).
Uniprot:	Q9BRR6
RRID:	AB_2834308

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(83%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ADPGK Antibody detects endogenous levels of total ADPGK.

RRID:

AB_2834308
Cite Format: Affinity Biosciences Cat# AF0517, RRID:AB_2834308.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2610017G09Rik; ADP dependent glucokinase; ADP GK; ADP-dependent glucokinase; ADP-GK; ADPGK; ADPGK_HUMAN; ATP dependent glucokinase; DKFZP434B195; RbBP 35; RbBP-35;

Immunogens

Immunogen:

Uniprot:

Q9BRR6(ADPGK_HUMAN) >>Visit HPA database.

Gene(ID):

ADPGK(83440)

Description:

ADPGK Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency. Belongs to the ADP-dependent glucokinase family. Monomer. 6 isoforms of the human protein are produced by alternative splicing.

Sequence:

Q9BRR6 ADPGK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALWRGSAYAGFLALAVGCVFLLEPELPGSALRSLWSSLCLGPAPAPPGPVSPEGRLAAAWDALIVRPVRRWRRVAVGVNACVDVVLSGVKLLQALGLSPGNGKDHSILHSRNDLEEAFIHFMGKGAAAERFFSDKETFHDIAQVASEFPGAQHYVGGNAALIGQKFAANSDLKVLLCGPVGPKLHELLDDNVFVPPESLQEVDEFHLILEYQAGEEWGQLKAPHANRFIFSHDLSNGAMNMLEVFVSSLEEFQPDLVVLSGLHMMEGQSKELQRKRLLEVVTSISDIPTGIPVHLELASMTNRELMSSIVHQQVFPAVTSLGLNEQELLFLTQSASGPHSSLSSWNGVPDVGMVSDILFWILKEHGRSKSRASDLTRIHFHTLVYHILATVDGHWANQLAAVAAGARVAGTQACATETIDTSRVSLRAPQEFMTSHSEAGSRIVLNPNKPVVEWHREGISFHFTPVLVCKDPIRTVGLGDAISAEGLFYSEVHPHY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Pig

Bovine

Sheep

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BRR6 As Substrate

Q9BRR6

Site	PTM Type	Source
K104	Ubiquitination	Uniprot
K125	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
K166	Ubiquitination	Uniprot
K370	Acetylation	Uniprot
T412	Phosphorylation	Uniprot
T417	Phosphorylation	Uniprot
S423	Phosphorylation	Uniprot
K450	Ubiquitination	Uniprot

Research Backgrounds

Q9BRR6_ADPGK_HUMAN

Function:

Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

Subcellular Location:

Secreted.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the ADP-dependent glucokinase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ADPGK Antibody - #AF0517